Recombinant Phospholipase D (PLD)

PLD1; Choline Phosphatase 1; Phosphatidylcholine-hydrolyzing phospholipase D1

  • Product No.RPC049Hu01
  • Organism SpeciesHomo sapiens (Human) Same name, Different species.
  • SourceProkaryotic expression
  • HostE.coli
  • Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
  • Subcellular LocationMembrane, Cytoplasm, Golgi apparatus, Endoplasmic reticulum lumen
  • Predicted Molecular Mass43.4kDa
  • Accurate Molecular Mass43kDa(Analysis of differences refer to the manual)
  • Residues & TagsThr725~Thr1074 with N-terminal His Tag
  • Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
  • Traits Freeze-dried powder
  • Purity> 90%
  • Isoelectric Point5.7
  • Applications Positive Control; Immunogen; SDS-PAGE; WB.
    If bio-activity of the protein is needed, please check active protein.
  • DownloadInstruction Manual
  • UOM 10µg50µg 200µg 1mg 5mg
  • FOB US$ 171  US$ 428  US$ 856  US$ 2568  US$ 6420 
    For more details, please contact local distributors!

SEQUENCE

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
American Journal of Physiology-Endocrinology and Metabolism Impact of glycosylphosphatidylinositol-specific phospholipase D on hepatic diacylglycerol accumulation, steatosis, and insulin resistance in diet-induced obesity Pubmed: 30457913
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPC049Hu01 Recombinant Phospholipase D (PLD) Positive Control; Immunogen; SDS-PAGE; WB.
PAC049Hu01 Polyclonal Antibody to Phospholipase D (PLD) WB; IHC; ICC; IP.
MAC049Hu22 Monoclonal Antibody to Phospholipase D (PLD) WB; IHC; ICC; IP.
SEC049Hu ELISA Kit for Phospholipase D (PLD) Enzyme-linked immunosorbent assay for Antigen Detection.
SCC049Hu CLIA Kit for Phospholipase D (PLD) Chemiluminescent immunoassay for Antigen Detection.
LMC049Hu Multiplex Assay Kit for Phospholipase D (PLD) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.