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Recombinant Cluster Of Differentiation 73 (CD73)

NT5E; NT5-E; E5NT; E5-NT; NTE; EN; ENT; 5'-Nucleotidase, Ecto

  • Product No.RPB250Bo01
  • Organism SpeciesBos taurus; Bovine (Cattle) Same name, Different species.
  • SourceProkaryotic expression
  • HostE.coli
  • Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
  • Subcellular LocationMembrane
  • Predicted Molecular Mass56.4kDa
  • Accurate Molecular Mass56kDa(Analysis of differences refer to the manual)
  • Residues & TagsSer48~Val527 with N-terminal His Tag
  • Buffer Formulation100mMNaHCO3, 500mMNaCl, pH8.3, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
  • Traits Freeze-dried powder
  • Purity> 95%
  • Isoelectric Point6.7
  • Applications Positive Control; Immunogen; SDS-PAGE; WB.
    If bio-activity of the protein is needed, please check active protein.
  • DownloadInstruction Manual
  • UOM 10µg50µg 200µg 1mg 5mg
  • FOB US$ 192  US$ 480  US$ 960  US$ 2880  US$ 7200 
    For more details, please contact local distributors!

SEQUENCE

USAGE

Reconstitute in 100mM NaHCO3, 500mM NaCl (pH8.3) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Journal of Autoimmunity The expression of CD39 on regulatory T cells is genetically driven and further upregulated at sites of inflammation Pubmed:25640206
Talanta Simultaneous accurate quantification of HO-1, CD39, and CD73 in human calcified aortic valves using multiple enzyme digestion–filter aided sample pretreatment … 10.1016:j.talanta.2018.01.044
Cancer Biomarkers Expression and clinical significance of serum NT5E protein in patients with colorectal cancer Pubmed: 30932882
Catalog No. Related products for research use of Bos taurus; Bovine (Cattle) Organism species Applications (RESEARCH USE ONLY!)
RPB250Bo01 Recombinant Cluster Of Differentiation 73 (CD73) Positive Control; Immunogen; SDS-PAGE; WB.
PAB250Bo01 Polyclonal Antibody to Cluster Of Differentiation 73 (CD73) WB; IHC; ICC; IP.