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Recombinant Alkaline Sphingomyelinase (Alk-Smase)

ENPP7; E-NPP 7; NPP-7; Ectonucleotide Pyrophosphatase/Phosphodiesterase 7; Sphingomyelin Phosphodiesterase; Intestinal alkaline sphingomyelinase

  • Product No.RPA801Ra01
  • Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
  • SourceProkaryotic expression
  • HostE.coli
  • Endotoxin Level<1.0EU per 1μg (determined by the LAL method).
  • Subcellular LocationMembrane
  • Predicted Molecular Mass51.5kDa
  • Accurate Molecular Mass51kDa(Analysis of differences refer to the manual)
  • Residues & TagsGly22~Val439 with N-terminal His Tag
  • Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
  • Traits Freeze-dried powder
  • Purity> 95%
  • Isoelectric Point6.6
  • Applications Positive Control; Immunogen; SDS-PAGE; WB.
    If bio-activity of the protein is needed, please check active protein.
  • DownloadInstruction Manual
  • UOM 10µg50µg 200µg 1mg 5mg
  • FOB US$ 178  US$ 446  US$ 892  US$ 2676  US$ 6690 
    For more details, please contact local distributors!

SEQUENCE

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Gastrointestinal and Liver Physiology Pretreatment with the probiotic VSL#3 delays transition from inflammation to dysplasia in a rat model of colitis-associated cancer PubMed: 21903764
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
RPA801Ra01 Recombinant Alkaline Sphingomyelinase (Alk-Smase) Positive Control; Immunogen; SDS-PAGE; WB.
PAA801Ra01 Polyclonal Antibody to Alkaline Sphingomyelinase (Alk-Smase) WB; IHC; ICC; IP.
MAA801Ra21 Monoclonal Antibody to Alkaline Sphingomyelinase (Alk-Smase) WB; IHC; ICC; IP.
SEA801Ra ELISA Kit for Alkaline Sphingomyelinase (Alk-Smase) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA801Ra Multiplex Assay Kit for Alkaline Sphingomyelinase (Alk-Smase) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.