Packages (Simulation)

Polyclonal Antibody to Specificity Protein 1 (Sp1)

Image (I)

DAB staining on IHC-P; Sample: Human Colon Tissue; Primary Ab: 20μg/ml Rabbit Anti-Human Sp1 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Small intestine Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Human Sp1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Lymph node Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Human Sp1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Spleen Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Human Sp1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Kidney Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Human Sp1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Lymph node Tissue
Primary Ab: 20µg/ml Rabbit Anti-Human Sp1 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Kidney Tissue
Primary Ab: 20µg/ml Rabbit Anti-Human Sp1 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Spleen Tissue
Primary Ab: 20µg/ml Rabbit Anti-Human Sp1 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
FITC staining on IF;
Sample: Human Hela cell;
Primary Ab: 30ug/ml Rabbit Anti-Human Sp1 Antibody
Second Ab: 2µg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb18)
FITC staining on IF;
Sample: Hela cell
Primary Ab: 30µg/ml Rabbit Anti-Human Sp1 Antibody
Second Ab: 2µg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb11)

Image (II)

Western Blot; Sample: HaCaT cell lysate Primary Ab: 1µg/ml Rabbit Anti-Human Sp1 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)

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Instruction Manual

Polyclonal-Antibody-to-Specificity-Protein-1-(Sp1)-PAM264Hu01.pdf

Certificate

Polyclonal Antibody to Specificity Protein 1 (Sp1)

TSFP1; Sp1 Transcription Factor

Product No.PAM264Hu01
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
SourcePolyclonal antibody preparation
HostRabbit
Potencyn/a
Ig Type IgG
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPM264Hu01-Recombinant Specificity Protein 1 (Sp1)
Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
TraitsLiquid
Concentration0.5mg/mL
Organism Species MoreSus scrofa; Porcine (Pig)
ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
DownloadInstruction Manual
UOM 20µl100µl 200µl 1ml 10ml
FOB US$ 116 270 US$ 270 US$ 386 US$ 965 US$ 3860
For more details, please contact local distributors!

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against Sp1. It has been selected for its ability to recognize Sp1 in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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INCREMENT SERVICES

Magazine	Citations
Scientific Reports	microRNA-29b mediates fibrotic induction of human xylosyltransferase-I in human dermal fibroblasts via the Sp1 pathway Pubmed: 30542210

Catalog No.	Related products for research use of Homo sapiens (Human) Organism species	Applications (RESEARCH USE ONLY!)
RPM264Hu01	Recombinant Specificity Protein 1 (Sp1)	Positive Control; Immunogen; SDS-PAGE; WB.
PAM264Hu01	Polyclonal Antibody to Specificity Protein 1 (Sp1)	WB; IHC; ICC; IP.
HEM264Hu	High Sensitive ELISA Kit for Specificity Protein 1 (Sp1)	Enzyme-linked immunosorbent assay for Antigen Detection.
SEM264Hu	ELISA Kit for Specificity Protein 1 (Sp1)	Enzyme-linked immunosorbent assay for Antigen Detection.
LMM264Hu	Multiplex Assay Kit for Specificity Protein 1 (Sp1) ,etc. by FLIA (Flow Luminescence Immunoassay)	FLIA Kit for Antigen Detection.