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Polyclonal Antibody to R-Spondin 1 (RSPO1)

Image (I)

DAB staining on IHC-P; Samples: Human Rectum Tissue; Primary Ab: 20µg/ml Rabbit Anti-Human RSPO1 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Liver Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human RSPO1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Liver cancer Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human RSPO1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Kidney Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human RSPO1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Breast cancer Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human RSPO1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Mouse Liver Tissue;
Primary Ab: 20ug/ml Rabbit Anti-Human RSPO1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Mouse Cardiac Muscle Tissue;
Primary Ab: 20ug/ml Rabbit Anti-Human RSPO1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

Image (II)

Figure. Western Blot; Sample: Recombinant RSPO1, Human.

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Instruction Manual

Polyclonal-Antibody-to-R-Spondin-1-(RSPO1)-PAM171Hu01.pdf

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Polyclonal Antibody to R-Spondin 1 (RSPO1)

RSPONDIN; Roof plate-specific spondin-1

Product No.PAM171Hu01
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
SourcePolyclonal antibody preparation
HostRabbit
Potencyn/a
Ig Type IgG
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPM171Hu01-Recombinant R-Spondin 1 (RSPO1)
Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
TraitsLiquid
Concentration500µg/mL
Organism Species MoreMus musculus (Mouse)
ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
DownloadInstruction Manual
UOM 20µl100µl 200µl 1ml 10ml
FOB US$ 116 270 US$ 270 US$ 386 US$ 965 US$ 3860
For more details, please contact local distributors!

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against RSPO1. It has been selected for its ability to recognize RSPO1 in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.5-2µg/mL
Immunohistochemistry: 5-20µg/mL
Immunocytochemistry: 5-20µg/mL
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine	Citations
Scand J Rheumatol.?	The association of radiographic progression with serum R-spondin 1 (RSPO1) levels or Dickkopf-1 (DKK1)/RSPO1 ratios in rheumatoid arthritis patients: clinical evidence for reciprocal inhibition between DKK1 and RSPO1. Pubmed:25178409

Catalog No.	Related products for research use of Homo sapiens (Human) Organism species	Applications (RESEARCH USE ONLY!)
RPM171Hu02	Recombinant R-Spondin 1 (RSPO1)	Positive Control; Immunogen; SDS-PAGE; WB.
APM171Hu61	Active R-Spondin 1 (RSPO1)	Cell culture; Activity Assays.
EPM171Hu61	Eukaryotic R-Spondin 1 (RSPO1)	Positive Control; Immunogen; SDS-PAGE; WB.
RPM171Hu01	Recombinant R-Spondin 1 (RSPO1)	Positive Control; Immunogen; SDS-PAGE; WB.
PAM171Hu01	Polyclonal Antibody to R-Spondin 1 (RSPO1)	WB; IHC; ICC; IP.
MAM171Hu21	Monoclonal Antibody to R-Spondin 1 (RSPO1)	WB; IHC; ICC; IP.
SEM171Hu	ELISA Kit for R-Spondin 1 (RSPO1)	Enzyme-linked immunosorbent assay for Antigen Detection.
LMM171Hu	Multiplex Assay Kit for R-Spondin 1 (RSPO1) ,etc. by FLIA (Flow Luminescence Immunoassay)	FLIA Kit for Antigen Detection.