Packages (Simulation)

Polyclonal Antibody to Aconitase 1 (ACO1)

Image (I)

DAB staining on IHC-P; Samples: Human Kidney Tissue; Primary Ab: 10µg/ml Rabbit Anti-Human ACO1 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Lung cancer Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human ACO1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Liver Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Human ACO1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

Image (II)

Figure. Western Blot; Sample: Recombinant ACO1, Human.
Western Blot; Sample: Porcine Liver lysate;
Primary Ab: 2µg/ml Rabbit Anti-Human ACO1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Mouse Liver lysate;
Primary Ab: 2µg/ml Rabbit Anti-Human ACO1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Human HepG2 cell lysate;
Primary Ab: 2µg/ml Rabbit Anti-Human ACO1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Human 293T cell lysate;
Primary Ab: 2µg/ml Rabbit Anti-Human ACO1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Porcine Kidney lysate;
Primary Ab: 2µg/ml Rabbit Anti-Human ACO1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Human SiHa cell was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 20µg/ml rabbit Anti-human ACO1 Polyclonal Antibody (Catalog PAJ683Hu02, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1µg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).

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Instruction Manual

Polyclonal-Antibody-to-Aconitase-1-(ACO1)-PAJ683Hu02.pdf

Certificate

Polyclonal Antibody to Aconitase 1 (ACO1)

IREB1; IRP1; IREBP; Iron regulatory protein 1; Iron-responsive element-binding protein 1; Cytoplasmic aconitate hydratase; Citrate hydro-lyase; Ferritin repressor protein

Product No.PAJ683Hu02
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
SourcePolyclonal antibody preparation
HostRabbit
Potencyn/a
Ig Type IgG
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPJ683Hu02-Recombinant Aconitase 1 (ACO1)
Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
TraitsLiquid
Concentration500µg/mL
Organism Species MoreMus musculus (Mouse), Rattus norvegicus (Rat), Sus scrofa; Porcine (Pig)
ApplicationsWB; ICC; IHC-P; IHC-F; ELISA; FCM
If the antibody is used in flow cytometry, please check FCM antibodies.
DownloadInstruction Manual
UOM 20µl100µl 200µl 1ml 10ml
FOB US$ 105 245 US$ 245 US$ 350 US$ 875 US$ 3500
For more details, please contact local distributors!

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against ACO1. It has been selected for its ability to recognize ACO1 in immunohistochemical staining and western blotting.

USAGE

Western blotting: 1-5μg/mL
Immunocytochemistry in formalin fixed cells: 5-20μg/mL
Immunohistochemistry in formalin fixed frozen section: 5-20μg/mL
Immunohistochemistry in paraffin section: 5-20μg/mL
Enzyme-linked Immunosorbent Assay: 0.05-2μg/mL
Flow cytometry:20μg/ml;
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Catalog No.	Related products for research use of Homo sapiens (Human) Organism species	Applications (RESEARCH USE ONLY!)
RPJ683Hu02	Recombinant Aconitase 1 (ACO1)	Positive Control; Immunogen; SDS-PAGE; WB.
RPJ683Hu03	Recombinant Aconitase 1 (ACO1)	Positive Control; Immunogen; SDS-PAGE; WB.
RPJ683Hu04	Recombinant Aconitase 1 (ACO1)	Positive Control; Immunogen; SDS-PAGE; WB.
RPJ683Hu01	Recombinant Aconitase 1 (ACO1)	Positive Control; Immunogen; SDS-PAGE; WB.
PAJ683Hu04	Polyclonal Antibody to Aconitase 1 (ACO1)	WB; ICC; IHC-P; IHC-F; ELISA.
PAJ683Hu02	Polyclonal Antibody to Aconitase 1 (ACO1)	WB; ICC; IHC-P; IHC-F; ELISA; FCM
PAJ683Hu01	Polyclonal Antibody to Aconitase 1 (ACO1)	WB; ICC; IHC-P; IHC-F; ELISA; FCM
PAJ683Hu03	Polyclonal Antibody to Aconitase 1 (ACO1)	WB; IHC; ICC; IP; FCM
LAJ683Hu71	Biotin-Linked Polyclonal Antibody to Aconitase 1 (ACO1)	WB; IHC; ICC.
SEJ683Hu	ELISA Kit for Aconitase 1 (ACO1)	Enzyme-linked immunosorbent assay for Antigen Detection.
LMJ683Hu	Multiplex Assay Kit for Aconitase 1 (ACO1) ,etc. by FLIA (Flow Luminescence Immunoassay)	FLIA Kit for Antigen Detection.