Packages (Simulation)

Polyclonal Antibody to Nephronectin (NPNT)

Image (I)

DAB staining on IHC-P; Samples: Human Liver Tissue; Primary Ab: 10µg/ml Rabbit Anti-Human NPNT Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Liver cancer Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human NPNT Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Pancreatic cancer Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human NPNT Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Skin cancer Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human NPNT Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
AF488 staining on IF;
Sample: MCF7 cell
Primary Ab: 20µg/ml Rabbit Anti-Human NPNT Antibody
Second Ab: 2μg/ml AF488-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb11)

Image (II)

Figure. Western Blot; Sample: Recombinant NPNT, Human.

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Instruction Manual

Polyclonal-Antibody-to-Nephronectin-(NPNT)-PAH522Hu01.pdf

Certificate

Polyclonal Antibody to Nephronectin (NPNT)

EGFL6L; POEM; Preosteoblast EGF-like repeat protein with MAM domain; Protein EGFL6-like

Product No.PAH522Hu01
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
SourcePolyclonal antibody preparation
HostRabbit
Potencyn/a
Ig Type IgG
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPH522Hu01-Recombinant Nephronectin (NPNT)
Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
TraitsLiquid
Concentration500µg/mL
Organism Species MoreMus musculus (Mouse)
ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
DownloadInstruction Manual
UOM 20µl100µl 200µl 1ml 10ml
FOB US$ 105 245 US$ 245 US$ 350 US$ 875 US$ 3500
For more details, please contact local distributors!

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against NPNT. It has been selected for its ability to recognize NPNT in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine	Citations
European Journal Of Human Genetics	Nephronectin (NPNT) and the prediction of nephrotic syndrome response to steroid treatment Pubmed:29891875

Catalog No.	Related products for research use of Homo sapiens (Human) Organism species	Applications (RESEARCH USE ONLY!)
APH522Hu01	Active Nephronectin (NPNT)	Cell culture; Activity Assays.
RPH522Hu02	Recombinant Nephronectin (NPNT)	Positive Control; Immunogen; SDS-PAGE; WB.
RPH522Hu03	Recombinant Nephronectin (NPNT)	Positive Control; Immunogen; SDS-PAGE; WB.
RPH522Hu01	Recombinant Nephronectin (NPNT)	Positive Control; Immunogen; SDS-PAGE; WB.
PAH522Hu01	Polyclonal Antibody to Nephronectin (NPNT)	WB; IHC; ICC; IP.
PAH522Hu02	Polyclonal Antibody to Nephronectin (NPNT)	WB; IHC; ICC; IP.
LAH522Hu71	Biotin-Linked Polyclonal Antibody to Nephronectin (NPNT)	WB; IHC; ICC.
SEH522Hu	ELISA Kit for Nephronectin (NPNT)	Enzyme-linked immunosorbent assay for Antigen Detection.
LMH522Hu	Multiplex Assay Kit for Nephronectin (NPNT) ,etc. by FLIA (Flow Luminescence Immunoassay)	FLIA Kit for Antigen Detection.