Packages (Simulation)

Polyclonal Antibody to Janus Kinase 2 (JAK2)

Image (I)

DAB staining on IHC-P; Samples: Human Liver cancer Tissue; Primary Ab: 30µg/ml Rabbit Anti-Human JAK2 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Kidney Tissue;
Primary Ab: 30µg/ml Rabbit Anti-Human JAK2 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Porcine Lymph node Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Human JAK2 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Porcine Kidney Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Human JAK2 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
FITC staining on IF;
Sample: Human Hela cell;
Primary Ab: 20μg/ml Rabbit Anti-Human JAK2 Antibody
Second Ab: 1.5μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb18)
FITC staining on IF;
Sample: Hela cell
Primary Ab: 20µg/ml Rabbit Anti-Human JAK2 Antibody
Second Ab: 2µg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb11)

Image (II)

Figure. Western Blot; Sample: Recombinant JAK2, Human.
Western Blot; Sample: Lane1: Human Lung lysate; Lane2: Jurkat cell lysate
Primary Ab: 3ug/ml Rabbit Anti-Human JAK2 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Knockout Varification:
Lane 1: Wild-type Jurkat cell lysate;
Lane 2: JAK2 knockout Jurkat cell lysate;
Predicted MW: 130kDa
Observed MW: 130kDa
Primary Ab: 3µg/ml Rabbit Anti-Human JAK2 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

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Instruction Manual

Polyclonal-Antibody-to-Janus-Kinase-2-(JAK2)-PAF494Hu01.pdf

Certificate

KO-Validated

Polyclonal Antibody to Janus Kinase 2 (JAK2)

JTK10; Tyrosine-protein kinase JAK2

Product No.PAF494Hu01
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
SourcePolyclonal antibody preparation
HostRabbit
Potencyn/a
Ig Type IgG
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPF494Hu01-Recombinant Janus Kinase 2 (JAK2)
Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
TraitsLiquid
Concentration0.5mg/ml
Organism Species MoreSus scrofa; Porcine (Pig)
ApplicationsWB; IHC; ICC/IF
If the antibody is used in flow cytometry, please check FCM antibodies.
DownloadInstruction Manual
UOM 20µl100µl 200µl 1ml 10ml
FOB US$ 105 245 US$ 245 US$ 350 US$ 875 US$ 3500
For more details, please contact local distributors!

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against JAK2. It has been selected for its ability to recognize JAK2 in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.01-3µg/mL;
Immunohistochemistry: 5-30µg/mL;
Immunofluorescence:5-20µg/mL;
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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INCREMENT SERVICES

Magazine	Citations
Scientific Reports	Xiaoyaosan exerts anxiolytic-like effects by down-regulating the TNF-α/JAK2-STAT3 pathway in the rat hippocampus. pubmed:28336920
Molecular Neurobiology	Erythropoietin Rescues Memory Impairment in a Rat Model of Chronic Cerebral Hypoperfusion via the EPO-R/JAK2/STAT5/PI3K/Akt/GSK-3β Pathway pubmed:28488208

Catalog No.	Related products for research use of Homo sapiens (Human) Organism species	Applications (RESEARCH USE ONLY!)
RPF494Hu01	Recombinant Janus Kinase 2 (JAK2)	Positive Control; Immunogen; SDS-PAGE; WB.
APF494Hu01	Active Janus Kinase 2 (JAK2)	Cell culture; Activity Assays.
PAF494Hu01	Polyclonal Antibody to Janus Kinase 2 (JAK2)	WB; IHC; ICC/IF
LAF494Hu71	Biotin-Linked Polyclonal Antibody to Janus Kinase 2 (JAK2)	WB; IHC; ICC.
SEF494Hu	ELISA Kit for Janus Kinase 2 (JAK2)	Enzyme-linked immunosorbent assay for Antigen Detection.