Packages (Simulation)

Image (I)

DAB staining on IHC-P; Samples: Human Mastadenoma Tissue; Primary Ab: 10µg/ml Rabbit Anti-Human NCL Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
Vector Red staining on IHC-P;
Samples: Human Tonsil Tissue;
Primary Ab: 15µg/ml Rabbit Anti-Human NCL Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
Vector Red staining on IHC-P;
Samples: Human Uterus Tissue;
Primary Ab: 15µg/ml Rabbit Anti-Human NCL Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody

Image (II)

Figure. Western Blot; Sample: Recombinant NCL, Human.
Figure. Western Blot; Sample: Human Lung Tissue.

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Instruction Manual

Polyclonal-Antibody-to-Nucleolin-(NCL)-PAC242Hu01.pdf

Certificate

Polyclonal Antibody to Nucleolin (NCL)

C23; Protein C23

Product No.PAC242Hu01
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
SourcePolyclonal antibody preparation
HostRabbit
Potencyn/a
Ig Type IgG
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPC242Hu01-Recombinant Nucleolin (NCL)
Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
TraitsLiquid
Concentration500µg/mL
Organism Species Moren/a
ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
DownloadInstruction Manual
UOM 20µl100µl 200µl 1ml 10ml
FOB US$ 105 245 US$ 245 US$ 350 US$ 875 US$ 3500
For more details, please contact local distributors!

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against NCL. It has been selected for its ability to recognize NCL in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.5-2µg/mL
Immunohistochemistry: 5-20µg/mL
Immunocytochemistry: 5-20µg/mL
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine	Citations
European Journal of Pharmaceutics and Biopharmaceutics	RNA G-quadruplex as supramolecular carrier for cancer-selective delivery
Mol Pharm	Drug Loading and Functional Efficacy of Cow, Buffalo, and Goat Milk-Derived Exosomes: A Comparative Study Pubmed:35195427
Frontiers in Physiology	Fine Tuning Inclusion in 2 of′-O-Methyl Phosphorothioate Oligonucleotides Contributes to Specific Cell Targeting for Splice-Switching Modulation Pubmed:34721051

Catalog No.	Related products for research use of Homo sapiens (Human) Organism species	Applications (RESEARCH USE ONLY!)
RPC242Hu01	Recombinant Nucleolin (NCL)	Positive Control; Immunogen; SDS-PAGE; WB.
RPC242Hu02	Recombinant Nucleolin (NCL)	Positive Control; Immunogen; SDS-PAGE; WB.
RPC242Hu03	Recombinant Nucleolin (NCL)	Positive Control; Immunogen; SDS-PAGE; WB.
PAC242Hu02	Polyclonal Antibody to Nucleolin (NCL)	WB; IHC; ICC; IP.
PAC242Hu03	Polyclonal Antibody to Nucleolin (NCL)	WB; IHC; ICC; IP.
PAC242Hu01	Polyclonal Antibody to Nucleolin (NCL)	WB; IHC; ICC; IP.
MAC242Hu22	Monoclonal Antibody to Nucleolin (NCL)	WB; IHC; ICC; IP.
SEC242Hu	ELISA Kit for Nucleolin (NCL)	Enzyme-linked immunosorbent assay for Antigen Detection.
LMC242Hu	Multiplex Assay Kit for Nucleolin (NCL) ,etc. by FLIA (Flow Luminescence Immunoassay)	FLIA Kit for Antigen Detection.