Packages (Simulation)

Polyclonal Antibody to Cyclin Dependent Kinase 4 (CDK4)

Image (I)

DAB staining on IHC-P; Samples: Human Lymphoma Tissue; Primary Ab: 10ug/ml Rabbit Anti-Human CDK4 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Breast cancer Tissue;
Primary Ab: 10ug/ml Rabbit Anti-Human CDK4 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Lung cancer Tissue;
Primary Ab: 10ug/ml Rabbit Anti-Human CDK4 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Breast cancer Tissue
Primary Ab: 10µg/ml Rabbit Anti-Human CDK4 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Lymphoma Tissue
Primary Ab: 10µg/ml Rabbit Anti-Human CDK4 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Lung cancer Tissue
Primary Ab: 10µg/ml Rabbit Anti-Human CDK4 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

Image (II)

Figure. Western Blot; Sample: Recombinant CDK4, Human.
Western Blot; Sample: Lane1: Hela cell lysate; Lane2: MCF7 cell lysate; Lane3: Rat Kidney lysate
Primary Ab: 1ug/ml Rabbit Anti-Human CDK4 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Knockout Varification:
Lane 1: Wild-type Hela cell lysate;
Lane 2: CDK4 knockout Hela cell lysate;
Predicted MW: 33kDa
Observed MW: 33kDa
Primary Ab: 1µg/ml Rabbit Anti-Human CDK4 Ab
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

Quality Guarantee

Instruction Manual

Polyclonal-Antibody-to-Cyclin-Dependent-Kinase-4-(CDK4)-PAB233Hu01.pdf

Certificate

KO-Validated

Polyclonal Antibody to Cyclin Dependent Kinase 4 (CDK4)

CMM3; PSK-J3; Cell division protein kinase 4

Product No.PAB233Hu01
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
SourcePolyclonal antibody preparation
HostRabbit
Potencyn/a
Ig Type IgG
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPB233Hu01-Recombinant Cyclin Dependent Kinase 4 (CDK4)
Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
TraitsLiquid
Concentration0.5mg/ml
Organism Species MoreRattus norvegicus (Rat)
ApplicationsWB,IHC
If the antibody is used in flow cytometry, please check FCM antibodies.
DownloadInstruction Manual
UOM 20µl100µl 200µl 1ml 10ml
FOB US$ 100 232 US$ 232 US$ 332 US$ 830 US$ 3320
For more details, please contact local distributors!

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against CDK4. It has been selected for its ability to recognize CDK4 in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

GIVEAWAYS

INCREMENT SERVICES

Catalog No.	Related products for research use of Homo sapiens (Human) Organism species	Applications (RESEARCH USE ONLY!)
RPB233Hu01	Recombinant Cyclin Dependent Kinase 4 (CDK4)	Positive Control; Immunogen; SDS-PAGE; WB.
PAB233Hu01	Polyclonal Antibody to Cyclin Dependent Kinase 4 (CDK4)	WB,IHC
MAB233Hu22	Monoclonal Antibody to Cyclin Dependent Kinase 4 (CDK4)	WB; IHC; ICC; IP.
SEB233Hu	ELISA Kit for Cyclin Dependent Kinase 4 (CDK4)	Enzyme-linked immunosorbent assay for Antigen Detection.
LMB233Hu	Multiplex Assay Kit for Cyclin Dependent Kinase 4 (CDK4) ,etc. by FLIA (Flow Luminescence Immunoassay)	FLIA Kit for Antigen Detection.