Polyclonal Antibody to Osteopontin (OPN)
SPP1; BNSP; BSPI; ETA1; Secreted Phosphoprotein 1; Bone Sialoprotein I; Early T-Lymphocyte Activation 1; Nephropontin; Urinary stone protein; Uropontin
- Product No.PAA899Hu02
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- SourcePolyclonal antibody preparation
- Ig Type IgG
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- Immunogen RPA899Hu02-Recombinant Osteopontin (OPN)
- Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
- Organism Species Moren/a
- ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
- DownloadInstruction Manual
200µl 1ml 10ml
For more details, please contact local distributors! US$ 153 FREE TRIAL
For more details, please contact local distributors! US$ 218 FREE TRIAL
For more details, please contact local distributors! US$ 545 FREE TRIAL
For more details, please contact local distributors! US$ 2180 FREE TRIAL
For more details, please contact local distributors!
The antibody is a rabbit polyclonal antibody raised against OPN. It has been selected for its ability to recognize OPN in immunohistochemical staining and western blotting.
Western blotting: 0.5-2µg/mL
Optimal working dilutions must be determined by end user.
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
- Antibody Labeling Customized Service
- Protein A/G Purification Column
- Staining Solution for Cells and Tissue
- Positive Control for Antibody
- Tissue/Sections Customized Service
- Phosphorylated Antibody Customized Service
- Immunohistochemistry (IHC) Experiment Service
- Immunocytochemistry (ICC) Experiment Service
- Flow Cytometry (FCM) Experiment Service
- Immunoprecipitation (IP) Experiment Service
- Immunofluorescence (IF) Experiment Service
- PBS Buffer
- DAB Kit
- SABC Kit
- Long-arm Biotin Labeling Kit
|Journal of Biomedical Materials Research Part A||Proliferation and osteogenesis of immortalized bone marrow-derived mesenchymal stem cells in porous polylactic glycolic acid scaffolds under perfusion culture PubMed: 19280635|
|Osteoarthritis and Cartilage||Elevated osteopontin level of synovial fluid and articular cartilage is associated with disease severity in knee osteoarthritis patients PubMed: 19747583|
|The Journal of Rheumatology||Thrombin-cleaved osteopontin levels in synovial fluid correlate with disease severity of knee osteoarthritis. Jrheum: source|
|International Journal of Pharmaceutics||Suppression of tumor growth in xenograft model mice by small interfering RNA targeting osteopontin delivery using biocompatible poly(amino ester) ScienceDirect: S0378517312003742|
|European Cells and matreials||The use of non-viral gene vectors for bioactive poly-(D, L-lactide) implant surfaces in bone tissue engineering Ecmjournal: Source|
|Journal of Equine Veterinary Science||Influence of Seminal Plasma Antioxidants and Osteopontin on Fertility of the Arabian Horse ScienceDirect: Source|
|Basic & Clinical Pharmacology & Toxicology||Treatment with Carnitine Enhances Bone Fracture Healing under Osteoporotic and/or Inflammatory Conditions Wiley:Source|
|Journal of Cellular Biochemistry||Tension Force-Induced ATP Promotes Osteogenesis Through P2X7 Receptor in Osteoblasts Pubmed:24905552|
|Naunyn-Schmiedeberg's Archives of Pharmacology||Treatment with α-lipoic acid enhances the bone healing after femoral fracture model of rats Pubmed:25038619|
|J Endocrinol Invest.?||The interplay among iron metabolism, endothelium and inflammatory cascade in dysmetabolic disorders Pubmed:25245337|
|FEBS Letters||Low-intensity pulsed ultrasound-induced ATP increases bone formation via the P2X7 receptor in osteoblast-like MC3T3-E1 cells Pubmed:25542352|
|Gene||Effects of magnesium degradation products on mesenchymal stem cell fate and osteoblastogenesis PubMed: 26283150|
|Medicine (Baltimore)||An Attempt to Evaluate Selected Aspects of “Bone–Fat Axis” Function in Healthy Individuals and Patients With Pancreatic Cancer PubMed: 26266370|
|Journal of Biomaterials and Tissue Engineering||Osteogenic Differentiation of Canine Adipose Stem Cells Cultured in Alginate-Fibrin-Based Hydrogel Content: Asp|
|Primasari||Osteopontin level correlates negatively with tumor shrinkage in neoadjuvant chemoradiation of locally advanced rectal cancer Index.Php: Amor|
|Int J Biol Sci||Defective Initiation of Liver Regeneration in Osteopontin-Deficient Mice after Partial Hepatectomy due to Insufficient Activation of IL-6/Stat3 Pathway PubMed: 26327817|
|Journal of Kunming Medical University / Kunming Yike Daxue Xuebao||Perioperative Clinical Investigation of Serum VEGF, CA19-9 and OPN Levels in Patients with Colorectal Carcinoma. Ebscohost|
|Am J Pathol.||Serum Osteopontin as a Novel Biomarker for Muscle Regeneration in Duchenne Muscular Dystrophy. Pubmed:26963343|
|circulation journal||Stenotic Bicuspid and Tricuspid Aortic Valves - Micro-Computed Tomography and Biological Indices of Calcification. pubmed:28344201|
|Journal of Cardiovascular Electrophysiology||Effect of Cardiac Resynchronization Therapy on Myocardial Fibrosis and Relevant Cytokines in a Canine Model With Experimental Heart Failure. pubmed:28127817|
|American Journal of Translational Research||Osteopontin stimulates matrix metalloproteinase expression through the nuclear factor-κB signaling pathway in rat temporomandibular joint and condylar chondrocytes. pubmed:28337262|
|Diabetes Care||Targeted Microbiome Intervention by Microencapsulated Delayed-Release Niacin Beneficially Affects Insulin Sensitivity in Humans pubmed:29212824|
|Biomedicine & pharmacotherapy||Icaritin induces MC3T3-E1 subclone14 cell differentiation through estrogen receptor-mediated ERK1/2 and p38 signaling activation. pubmed:28742995|
|Catalog No.||Related products for research use of Homo sapiens (Human) Organism species||Applications (RESEARCH USE ONLY!)|
|EPA899Hu61||Eukaryotic Osteopontin (OPN)||Positive Control; Immunogen; SDS-PAGE; WB.|
|RPA899Hu02||Recombinant Osteopontin (OPN)||Positive Control; Immunogen; SDS-PAGE; WB.|
|APA899Hu61||Active Osteopontin (OPN)||Cell culture; Activity Assays.|
|PAA899Hu06||Polyclonal Antibody to Osteopontin (OPN)||WB; IHC; ICC; IP.|
|PAA899Hu02||Polyclonal Antibody to Osteopontin (OPN)||WB; IHC; ICC; IP.|
|LAA899Hu71||Biotin-Linked Polyclonal Antibody to Osteopontin (OPN)||WB; IHC; ICC.|
|MAA899Hu22||Monoclonal Antibody to Osteopontin (OPN)||WB; IHC; ICC; IP.|
|SEA899Hu||ELISA Kit for Osteopontin (OPN)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|SCA899Hu||CLIA Kit for Osteopontin (OPN)||Chemiluminescent immunoassay for Antigen Detection.|
|KSA899Hu01||ELISA Kit DIY Materials for Osteopontin (OPN)||Main materials for "Do It (ELISA Kit) Yourself".|