Mini Samples ELISA Kit for Beta-Crosslaps (bCTx)

b-CTx; bCTXI; bCTX-I; B-Cr; BCL; Type I Collagen C-Telopeptide-Related Fraction

Specificity

This assay has high sensitivity and excellent specificity for detection of Mini Samples Beta-Crosslaps (bCTx).
No significant cross-reactivity or interference between Mini Samples Beta-Crosslaps (bCTx) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Mini Samples Beta-Crosslaps (bCTx) and the recovery rates were calculated by comparing the measured value to the expected amount of Mini Samples Beta-Crosslaps (bCTx) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 80-103 99
EDTA plasma(n=5) 80-99 79
heparin plasma(n=5) 78-98 94

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Beta-Crosslaps (bCTx) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Beta-Crosslaps (bCTx) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Mini Samples Beta-Crosslaps (bCTx) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 87-94% 96-104% 78-104% 80-95%
EDTA plasma(n=5) 96-105% 93-104% 78-95% 79-93%
heparin plasma(n=5) 79-105% 79-90% 80-103% 96-103%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×60µL Assay Diluent A 1×6mL
Detection Reagent B 1×60µL Assay Diluent B 1×6mL
TMB Substrate 1×9mL Stop Solution 1×3mL
Wash Buffer (30 × concentrate) 1×10mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 25µL standard or sample to each well.
    And then add 25μL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 50µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 50µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 25µL Stop Solution. Read at 450 nm immediately.

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Bone Elevated serum soluble CD200 and CD200R as surrogate markers of bone loss under bed rest conditions Pubmed:24333170
J Bone Miner Metab. Effect of Urocortin on strength and microarchitecture of osteopenic rat femur Pubmed:24633537
Journal of Nutrition Cholecalciferol Supplementation Promotes Bone Turnover in Chinese Adults with Vitamin D Deficiency Pubmed:29897564
Journal of Functional Foods Effect of long-term administration of mangiferin from on bone metabolism in ovariectomized rats 10.1016:j.jff.2018.04.048
European Journal of Medical Research Biomechanical and histological analyses of the fracture healing process after direct or prolonged reduction Pubmed: 30180907
Endokrynologia Polska FGF23 and primary hyperparathyroidism: is there a link? Pubmed: 32598019
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