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Monoclonal Antibody to Milk Fat Globule EGF Factor 8 (MFGE8)

HMFG; MFGM; SED1; BA46; EDIL1; OAcGD3S; SPAG10; hP47; Medin; Lactadherin; Sperm Associated Antigen 10; Sperm Surface Protein hP47; Milk fat globule-EGF factor 8

  • Product No.MAB286Hu22
  • Organism SpeciesHomo sapiens (Human) Same name, Different species.
  • SourceMonoclonal antibody preparation
  • HostMouse
  • Potency (Clone Number)n/a
  • Ig Isotype IgG
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen n/a
  • Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
  • TraitsLiquid
  • Concentration1mg/mL
  • Organism Species Moren/a
  • ApplicationsWB; IHC; ICC; IP.
    If the antibody is used in flow cytometry, please check FCM antibodies.
  • Downloadn/a
  • UOM 20µl100µl 200µl 1ml 10ml
  • FOB US$ 106  US$ 246  US$ 352  US$ 880  US$ 3520 
    For more details, please contact local distributors!

SPECIFITY

The antibody is a mouse monoclonal antibody raised against MFGE8. It has been selected for its ability to recognize MFGE8 in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.2-2µg/mL;1:500-5000
Immunohistochemistry: 5-20µg/mL;1:50-200
Immunocytochemistry: 5-20µg/mL;1:50-200
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Fertility and Sterility Tumor necrosis factor α up-regulates endometrial milk fat globule-epidermal growth factor 8 protein production via nuclear factor κB activation, resulting in cell migration of epithelial cells. Pubmed: 24262600
U.S. Patent Application Biomarker for amyloid-b-related neurological disorders US20130302838
IUBMB Life. Elevated serum milk fat globule-epidermal growth factor 8 levels in type 2 diabetic patients are suppressed by overweight or obese status. pubmed:28067023
Journal of diabetes investigation Circulating milk fat globule-epidermal growth factor 8 levels are increased in pregnancy and gestational diabetes mellitus. pubmed:28035763
The Journal of Steroid Biochemistry and Molecular Biology Modifications of Western-type diet regarding protein, fat and sucrose levels as modulators of steroid metabolism and activity in liver pubmed:27471150
Journal of Steroid Biochemistry and Molecular Biology Depressive-like effect of prenatal exposure to DDT involves global DNA hypomethylation and impairment of GPER1/ESR1 protein levels but not ESR2 and AHR/ARNT signaling pubmed:28263910
Oncotarget Pantoprazole blocks the JAK2/STAT3 pathway to alleviate skeletal muscle wasting in cancer cachexia by inhibiting inflammatory response. pubmed:28489606
Molecular and Cellular Neuroscience TGF-β1 enhances phagocytic removal of neuron debris and neuronal survival by olfactory ensheathing cells via integrin/MFG-E8 signaling pathway 10.1016/j.mcn.2017.08.006
Amyloid Needle-shaped amyloid deposition in rat mammary gland: evidence of a novel amyloid fibril protein Pubmed: 31615282
clinical and translational medicine Milk fat globule EGF factor 8 restores mitochondrial function via integrin©\medicated activation of the FAK©\STAT3 signaling pathway in acute pancreatitis 33634976
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPB286Hu01 Recombinant Milk Fat Globule EGF Factor 8 (MFGE8) Positive Control; Immunogen; SDS-PAGE; WB.
PAB286Hu01 Polyclonal Antibody to Milk Fat Globule EGF Factor 8 (MFGE8) WB; IHC; ICC; IP.
MAB286Hu22 Monoclonal Antibody to Milk Fat Globule EGF Factor 8 (MFGE8) WB; IHC; ICC; IP.
SEB286Hu ELISA Kit for Milk Fat Globule EGF Factor 8 (MFGE8) Enzyme-linked immunosorbent assay for Antigen Detection.
SCB286Hu CLIA Kit for Milk Fat Globule EGF Factor 8 (MFGE8) Chemiluminescent immunoassay for Antigen Detection.
LMB286Hu Multiplex Assay Kit for Milk Fat Globule EGF Factor 8 (MFGE8) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.