Multiplex Assay Kit for Interleukin 24 (IL24) ,etc. by FLIA (Flow Luminescence Immunoassay)

C49A; FISP; IL10B; MDA7; Mob-5; ST16; Mda-7; Melanoma Differentiation Association Protein 7; Suppression Of Tumorigenicity 16

(Note: Up to 8-plex in one testing reaction)

Specificity

This assay has high sensitivity and excellent specificity for detection of Interleukin 24 (IL24) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Interleukin 24 (IL24) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Interleukin 24 (IL24) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Interleukin 24 (IL24) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 83-97 88
EDTA plasma(n=5) 81-89 84
heparin plasma(n=5) 83-97 90

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 24 (IL24) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 24 (IL24) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Interleukin 24 (IL24) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 85-99% 78-91% 92-102% 85-97%
EDTA plasma(n=5) 93-101% 84-92% 98-105% 97-105%
heparin plasma(n=5) 84-101% 96-105% 85-102% 79-89%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:IL24) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
    add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

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Magazine Citations
Experimental & Clinical Cancer Research Oncolytic adenovirus armed with IL-24 Inhibits the growth of breast cancer in vitro and in vivo PubMed: PMC3511263
Bioresources and Bioprocessing Improving the mda-7/IL-24 refolding and purification process using optimized culture conditions based on the structure characteristics of inclusion bodies Springer:Source
Clin Exp Pharmacol Physiol. Inhibition of ALDH2 protects PC12 cells against formaldehyde-induced cytotoxicity: involving the protection of hydrogen sulphide. pubmed:28251688
Theranostics IL20RA signaling enhances stemness and promotes the formation of an immunosuppressive microenvironment in breast cancer 33456560
BIOLOGICAL CHEMISTRY IL-24 inhibits the malignancy of human glioblastoma cells via destabilization of Zeb1 33894112
J Transl Med Interleukin-24 regulates mucosal remodeling in inflammatory bowel diseases 34078403
Nutrients Effect of Serum Spermidine on the Prognosis in Patients with Acute Myocardial Infarction: A Cohort Study Pubmed:35406007
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
RPC064Ra01 Recombinant Interleukin 24 (IL24) Positive Control; Immunogen; SDS-PAGE; WB.
PAC064Ra01 Polyclonal Antibody to Interleukin 24 (IL24) WB; IHC; ICC; IP.
LAC064Ra71 Biotin-Linked Polyclonal Antibody to Interleukin 24 (IL24) WB; IHC; ICC.
MAC064Ra21 Monoclonal Antibody to Interleukin 24 (IL24) WB; IHC; ICC; IP.
MAC064Ra22 Monoclonal Antibody to Interleukin 24 (IL24) WB; IHC; ICC; IP.
MAC064Ra23 Monoclonal Antibody to Interleukin 24 (IL24) WB; IHC; ICC; IP.
MAC064Ra24 Monoclonal Antibody to Interleukin 24 (IL24) WB; IHC; ICC; IP.
MAC064Ra25 Monoclonal Antibody to Interleukin 24 (IL24) WB; IHC; ICC; IP.
SEC064Ra ELISA Kit for Interleukin 24 (IL24) Enzyme-linked immunosorbent assay for Antigen Detection.
SCC064Ra CLIA Kit for Interleukin 24 (IL24) Chemiluminescent immunoassay for Antigen Detection.
LMC064Ra Multiplex Assay Kit for Interleukin 24 (IL24) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.