Multiplex Assay Kit for Podocalyxin (PODXL) ,etc. by FLIA (Flow Luminescence Immunoassay)

Gp200; PCLP; PC; PCX; Podocalyxin Like Protein 1; GCTM-2 antigen

(Note: Up to 8-plex in one testing reaction)

Specificity

This assay has high sensitivity and excellent specificity for detection of Podocalyxin (PODXL) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Podocalyxin (PODXL) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Podocalyxin (PODXL) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Podocalyxin (PODXL) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:PODXL) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
    add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

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Magazine Citations
Egypt J Rheumatology and Clinical Immunology Urinary Podocalyxin and Lupus Nephritis Disease Activity 4473_583473a89b4f569662891fe3df447e45
OncoTargets and Therapy Measurement of serum PODXL concentration for detection of pancreatic cancer Pubmed:29588598
Menoufia Medical Journal Urinary podocalyxin and cyclophilin A: markers for early detection of type 2 diabetic nephropathy
European Journal of Obstetrics & Gynecology and Reproductive Biology The Role of Serum Podocalyxin Levels in Recurrent Pregnancy Loss 33765479
Life Sciences Vildagliptin alleviates liver fibrosis in NASH diabetic rats via modulation of insulin resistance, oxidative stress, and inflammatory cascades Pubmed:35671811
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPA768Mu01 Recombinant Podocalyxin (PODXL) Positive Control; Immunogen; SDS-PAGE; WB.
RPA768Mu02 Recombinant Podocalyxin (PODXL) Positive Control; Immunogen; SDS-PAGE; WB.
PAA768Mu02 Polyclonal Antibody to Podocalyxin (PODXL) WB; IHC; ICC; IP.
PAA768Mu01 Polyclonal Antibody to Podocalyxin (PODXL) WB; IHC; ICC; IP.
SEA768Mu ELISA Kit for Podocalyxin (PODXL) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA768Mu Multiplex Assay Kit for Podocalyxin (PODXL) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.