≡

Multiplex Assay Kit for Glucose-6-phosphate Dehydrogenase (G6PD) ,etc. by FLIA (Flow Luminescence Immunoassay)

Glucose-6-phosphate 1-dehydrogenase

(Note: Up to 8-plex in one testing reaction)

Specificity

This assay has high sensitivity and excellent specificity for detection of Glucose-6-phosphate Dehydrogenase (G6PD) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Glucose-6-phosphate Dehydrogenase (G6PD) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Glucose-6-phosphate Dehydrogenase (G6PD) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Glucose-6-phosphate Dehydrogenase (G6PD) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 88-101 94
EDTA plasma(n=5) 92-99 95
heparin plasma(n=5) 96-103 101

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glucose-6-phosphate Dehydrogenase (G6PD) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glucose-6-phosphate Dehydrogenase (G6PD) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Glucose-6-phosphate Dehydrogenase (G6PD) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 82-94% 81-101% 92-101% 88-96%
EDTA plasma(n=5) 79-101% 95-105% 79-97% 83-92%
heparin plasma(n=5) 81-94% 93-101% 85-97% 90-104%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:G6PD) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
    add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Artificial Organs Experimental Research With Synthetic Copolymer-Coated Cardiopulmonary Bypass Circuits: Inflammatory and Thrombogenicity Analysis Wiley: source
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
RPA716Ra01 Recombinant Glucose-6-phosphate Dehydrogenase (G6PD) Positive Control; Immunogen; SDS-PAGE; WB.
CPA716Ra21 OVA Conjugated Glucose-6-phosphate Dehydrogenase (G6PD) Immunogen; SDS-PAGE; WB.
PAA716Ra01 Polyclonal Antibody to Glucose-6-phosphate Dehydrogenase (G6PD) WB
LAA716Ra71 Biotin-Linked Polyclonal Antibody to Glucose-6-phosphate Dehydrogenase (G6PD) WB; IHC; ICC.
LAA716Ra81 FITC-Linked Polyclonal Antibody to Glucose-6-phosphate Dehydrogenase (G6PD) WB; IHC; ICC; IF.
MAA716Ra21 Monoclonal Antibody to Glucose-6-phosphate Dehydrogenase (G6PD) WB; IHC; ICC; IP.
SEA716Ra ELISA Kit for Glucose-6-phosphate Dehydrogenase (G6PD) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA716Ra Multiplex Assay Kit for Glucose-6-phosphate Dehydrogenase (G6PD) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.