Multiplex Assay Kit for Immunoglobulin E (IgE) ,etc. by FLIA (Flow Luminescence Immunoassay)

IGHE; Immunoglobulin Heavy Constant Epsilon; Ig epsilon chain C region

(Note: Up to 8-plex in one testing reaction)

Specificity

This assay has high sensitivity and excellent specificity for detection of Immunoglobulin E (IgE) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Immunoglobulin E (IgE) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Immunoglobulin E (IgE) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Immunoglobulin E (IgE) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 81-97 90
EDTA plasma(n=5) 85-94 91
heparin plasma(n=5) 84-99 90
sodium citrate plasma(n=5) 95-102 98

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Immunoglobulin E (IgE) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Immunoglobulin E (IgE) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Immunoglobulin E (IgE) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 98-105% 81-105% 94-102% 84-102%
EDTA plasma(n=5) 78-97% 85-103% 93-101% 85-104%
heparin plasma(n=5) 80-99% 79-104% 95-102% 94-105%
sodium citrate plasma(n=5) 80-89% 79-94% 96-103% 93-104%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:IgE) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
    add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Molecular Carcinogenesis IL17A gene polymorphisms, serum IL-17A and IgE levels, and hepatocellular carcinoma risk in patients with chronic hepatitis B virus infection PubMed: 23280722
Human Immunology IL21 and IL21R polymorphisms and their interactive effects on serum IL-21 and IgE levels in patients with chronic hepatitis B virus infection PubMed: 23354321
Scand J Immunol Passive transfer of tumour-derived MDSCs inhibits asthma-related airway inflammation Pubmed: 24313384
AAPS PharmSciTech Topical Lyogel Containing Corticosteroid Decreases IgE Expression and Enhances the Therapeutic Efficacy Against Atopic Eczema PubMed: 25511806
Ann Allergy Asthma Immunol Reduced airway microbiota diversity is associated with elevated allergic respiratory inflammation PubMed: 26123423
Molecular Medicine Reports The protective role of vitamin D3 in a murine model of asthma via the suppression of TGF-β/Smad signaling and activation of the Nrf2/HO-1 pathway. pubmed:27484042
Gut Pathog. Oral administration of Clostridium butyricum CGMCC0313‐1 reduces ovalbumin‐induced allergic airway inflammation in mice pubmed:28250847
International Immunopharmacology Catalpol alleviates ovalbumin-induced asthma in mice: Reduced eosinophil infiltration in the lung pubmed:27992791
Asian Pacific Society of Respirology Oral administration of Clostridium butyricum CGMCC0313-1 reduces ovalbumin-induced allergic airway inflammation in mice pubmed:28122397
Molecular Medicine Reports Protective effects of astragaloside IV against ovalbumin-induced allergic rhinitis are mediated by T-box protein expressed in T cells/GATA-3 and forkhead box protein 3/retinoic acid-related orphan nuclear receptor γt. pubmed:28586019
Journal of Autoimmunity Commensal bacteria aggravate allergic asthma via NLRP3/IL-1β signaling in post-weaning mice 10.1016:j.jaut.2018.07.003
Water Research  Sub-chronic inhalation of reclaimed water-induced fibrotic lesion in a mouse model Pubmed:29655095
Human & Experimental Toxicology Investigation of hypersensitivity potential of diacetyl by determining cytokine profiles Pubmed:29233007
Journal of International Medical Research Changes in Th1/Th2-producing cytokines during acute exacerbation chronic obstructive pulmonary disease Pubmed:29950127
Journal Of Cellular Biochemistry Effects of catalpol on bronchial asthma and its relationship with cytokines Pubmed: 30536454
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES  Adipose Tissue-Derived Mesenchymal Stem Cell Modulates the Immune Response of Allergic Rhinitis in a Rat Model Pubmed: 30781605
Royal Society Open Science Metabolomics analysis of baicalin on ovalbumin-sensitized allergic rhinitis rats
Health and Medicine Antiallergical Effect of New Combined Nazal Aerodisperse System in the Conditions of Experimental Allergic Rhinitis
American Journal of Translational Research Inhibitory effects of catalpol coordinated with budesonide and their relationship with cytokines and Interleukin-13 expression Pubmed: 31737193
MOLECULAR IMMUNOLOGY Animal models for analysis of hypersensitivity reactions to Shuanghuanglian injection Pubmed: 32302806
bioscience reports Protective effect of Asarum sieboldii essential oil on ovalbumin induced allergic rhinitis in rat Pubmed: 32395767
international journal of antimicrobial agents Synergistic Phage-surfactant Combination Clears IgE-promoted Staphylococcus aureus Aggregation in Vitro and Enhances the Effect in Vivo Pubmed: 32335278
Aggravation of Airway Inflammation in RSV-Infected Asthmatic Mice Following Infection-Induced Alteration of Gut Microbiota
ACS Biomaterials Science & Engineering Tissue-Engineered Decellularized Allografts for Anterior Cruciate Ligament Reconstruction
Biomarkers of Allergic Asthma and their Association with Serum Parameters
Biomed Pharmacother Qingfei oral liquid inhibited autophagy to alleviate inflammation via mTOR signaling pathway in RSV-infected asthmatic mice 33706133
Environ Pollut Trace endotoxin in reclaimed water is only one of the risk sources in subchronic inhalation exposure 34090073
Annals of Tropical Medicine & Public Health Effectiveness of biomarkers and serum parameters in determination allergic asthma and detection of its severity
Mediators Inflamm Roles Played by the PI3K/Akt/HIF-1α Pathway and IL-17A in the Chinese Subtype of Chronic Sinusitis with Nasal Polyps Pubmed:35075349
Expert Opinion on Therapeutic Targets Dapagliflozin mitigates ovalbumin-prompted airway inflammatory-oxidative successions and associated bronchospasm in a rat model of allergic asthma Pubmed:35549595
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
NPA545Mu01 Native Immunoglobulin E (IgE) Positive Control; Immunogen; SDS-PAGE; WB.
RPA545Mu01 Recombinant Immunoglobulin E (IgE) Positive Control; Immunogen; SDS-PAGE; WB.
RPA545Mu02 Recombinant Immunoglobulin E (IgE) Positive Control; Immunogen; SDS-PAGE; WB.
PAA545Mu01 Polyclonal Antibody to Immunoglobulin E (IgE) WB; IHC; ICC; IP.
PAA545Mu02 Polyclonal Antibody to Immunoglobulin E (IgE) WB; IHC; ICC; IP.
LAA545Mu71 Biotin-Linked Polyclonal Antibody to Immunoglobulin E (IgE) WB; IHC; ICC.
SEA545Mu ELISA Kit for Immunoglobulin E (IgE) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA545Mu CLIA Kit for Immunoglobulin E (IgE) Chemiluminescent immunoassay for Antigen Detection.
LMA545Mu Multiplex Assay Kit for Immunoglobulin E (IgE) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.