Multiplex Assay Kit for Caveolin 1 (CAV1) ,etc. by FLIA (Flow Luminescence Immunoassay)

VIP21; Caveolae Protein 22kDa

(Note: Up to 8-plex in one testing reaction)

Specificity

This assay has high sensitivity and excellent specificity for detection of Caveolin 1 (CAV1) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Caveolin 1 (CAV1) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Caveolin 1 (CAV1) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Caveolin 1 (CAV1) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 79-92 87
EDTA plasma(n=5) 87-95 91
heparin plasma(n=5) 90-101 93

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Caveolin 1 (CAV1) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Caveolin 1 (CAV1) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Caveolin 1 (CAV1) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 80-93% 87-105% 80-104% 82-98%
EDTA plasma(n=5) 91-98% 89-101% 80-89% 80-92%
heparin plasma(n=5) 88-101% 80-101% 94-104% 83-102%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:CAV1) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
    add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

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Magazine Citations
Mendel Net Novel prostate cancer tumour markers in a cell line model Mendelu: source
Oncology reports Caveolin-1 as a potential high-risk prostate cancer biomarker PubMed: 22159333
Anticancer Research Increased plasma caveolin-1 levels are associated with progression of prostate cancer among Japanese men Pubmed: 23645736
Sarcoidosis Vasc Diffuse Lung Dis.? Decrased sputum caveolin-1 is associated with systemic sclerosis related lung disease Pubmed:24751454
Cancer Genomics and Proteomics Significant Association of Caveolin-1 and Caveolin-2 with Prostate Cancer Progression PubMed: 26543085
Atherosclerosis Genomic variant in CAV1 increases susceptibility to coronary artery disease and myocardial infarction Pubmed:26775120
Arthritis Research & Therapy Increased caveolin-1 in intervertebral disc degeneration facilitates repair Pubmed:26939667
Oxid Med Cell Longev Lower Serum Caveolin-1 Is Associated with Cerebral Microbleeds in Patients with Acute Ischemic Stroke Pubmed:27119011
Inflammation Caveolin-1 Promotes the Imbalance of Th17/Treg in Patients with Chronic Obstructive PulmonaryDisease. pubmed:27613621
Oxidative Medicine and Cellular Longevity Swim Training Modulates Skeletal Muscle Energy Metabolism, Oxidative Stress, and Mitochondrial Cholesterol Content in Amyotrophic Lateral Sclerosis Mice Pubmed:29849903
Microcirculation Endothelium‐dependent hyperpolarization‐mediated vasodilatation compensates nitric oxide‐mediated endothelial dysfunction during ischemia in diabetes‐induced … Pubmed:29665152
Genetic Testing and Molecular Biomarkers The CAV1 Gene 3′ Untranslated Region Single Nucleotide Polymorphisms Are Associated with the Risk of Pulmonary Hypertension in Chinese Han Chronic …
Cell Rep SIRT6 transcriptionally regulates fatty acid transport by suppressing PPAR¦Ã 34077730
researchsquare Cav-1 Protein Levels in Serum and Infarcted Brain Correlate with Hemorrhagic Volume in a Mouse Model of Thromboembolic Stroke, Independently of Rt-PA?¡­
Molecules 5, 2′-Dibromo-2, 4′, 5′-trihydroxydiphenylmethanone Inhibits LPS-Induced Vascular Inflammation by Targeting the Cav1 Protein Pubmed:35566232
Catalog No. Related products for research use of Chicken (Gallus) Organism species Applications (RESEARCH USE ONLY!)
RPA214Ga01 Recombinant Caveolin 1 (CAV1) Positive Control; Immunogen; SDS-PAGE; WB.
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SEA214Ga ELISA Kit for Caveolin 1 (CAV1) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA214Ga Multiplex Assay Kit for Caveolin 1 (CAV1) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.