ELISA Kit for Caveolin 1 (CAV1)

VIP21; Caveolae Protein 22kDa

Specificity

This assay has high sensitivity and excellent specificity for detection of Caveolin 1 (CAV1).
No significant cross-reactivity or interference between Caveolin 1 (CAV1) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Caveolin 1 (CAV1) and the recovery rates were calculated by comparing the measured value to the expected amount of Caveolin 1 (CAV1) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 84-102 94
EDTA plasma(n=5) 84-94 90
heparin plasma(n=5) 88-98 94

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Caveolin 1 (CAV1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Caveolin 1 (CAV1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Caveolin 1 (CAV1) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 98-105% 83-94% 92-99% 98-105%
EDTA plasma(n=5) 82-91% 84-101% 88-102% 88-102%
heparin plasma(n=5) 81-104% 99-105% 80-102% 97-105%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

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Magazine Citations
Mendel Net Novel prostate cancer tumour markers in a cell line model Mendelu: source
Oncology reports Caveolin-1 as a potential high-risk prostate cancer biomarker PubMed: 22159333
Anticancer Research Increased plasma caveolin-1 levels are associated with progression of prostate cancer among Japanese men Pubmed: 23645736
Sarcoidosis Vasc Diffuse Lung Dis.? Decrased sputum caveolin-1 is associated with systemic sclerosis related lung disease Pubmed:24751454
Cancer Genomics and Proteomics Significant Association of Caveolin-1 and Caveolin-2 with Prostate Cancer Progression PubMed: 26543085
Atherosclerosis Genomic variant in CAV1 increases susceptibility to coronary artery disease and myocardial infarction Pubmed:26775120
Arthritis Research & Therapy Increased caveolin-1 in intervertebral disc degeneration facilitates repair Pubmed:26939667
Oxid Med Cell Longev Lower Serum Caveolin-1 Is Associated with Cerebral Microbleeds in Patients with Acute Ischemic Stroke Pubmed:27119011
Inflammation Caveolin-1 Promotes the Imbalance of Th17/Treg in Patients with Chronic Obstructive PulmonaryDisease. pubmed:27613621
Oxidative Medicine and Cellular Longevity Swim Training Modulates Skeletal Muscle Energy Metabolism, Oxidative Stress, and Mitochondrial Cholesterol Content in Amyotrophic Lateral Sclerosis Mice Pubmed:29849903
Microcirculation Endothelium‐dependent hyperpolarization‐mediated vasodilatation compensates nitric oxide‐mediated endothelial dysfunction during ischemia in diabetes‐induced … Pubmed:29665152
Genetic Testing and Molecular Biomarkers The CAV1 Gene 3′ Untranslated Region Single Nucleotide Polymorphisms Are Associated with the Risk of Pulmonary Hypertension in Chinese Han Chronic …
Cell Rep SIRT6 transcriptionally regulates fatty acid transport by suppressing PPAR¦Ã 34077730
researchsquare Cav-1 Protein Levels in Serum and Infarcted Brain Correlate with Hemorrhagic Volume in a Mouse Model of Thromboembolic Stroke, Independently of Rt-PA?¡­
Molecules 5, 2′-Dibromo-2, 4′, 5′-trihydroxydiphenylmethanone Inhibits LPS-Induced Vascular Inflammation by Targeting the Cav1 Protein Pubmed:35566232
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