Magnetic Luminex Assay Kit for Platelet Factor 4 (PF4) ,etc.
CXCL4; SCYB4; Chemokine C-X-C-Motif Ligand 4; Oncostatin-A; Iroplact
(Note: Up to 8-plex in one testing reaction)
- Product No.LMA172Mu
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- Sample TypeSerum, platelet-poor plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3.5h
- Detection Range0.01-10ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.003 ng/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
For more details, please contact local distributors! 670 US$ 670
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For more details, please contact local distributors! US$ 5695
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- Add to Cart (96T)
This assay has high sensitivity and excellent specificity for detection of Platelet Factor 4 (PF4) ,etc..
No significant cross-reactivity or interference between Platelet Factor 4 (PF4) ,etc. and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Platelet Factor 4 (PF4) ,etc. and the recovery rates were calculated by comparing the measured value to the expected amount of Platelet Factor 4 (PF4) ,etc. in samples.
|Matrix||Recovery range (%)||Average(%)|
|sodium citrate plasma(n=5)||94-103||98|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Platelet Factor 4 (PF4) ,etc. were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Platelet Factor 4 (PF4) ,etc. were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Platelet Factor 4 (PF4) ,etc. and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|sodium citrate plasma(n=5)||78-97%||95-102%||84-95%||96-105%|
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|96-well plate||1||Plate sealer for 96 wells||4|
|Pre-Mixed Standard||2||Standard Diluent||1×20mL|
|Pre-Mixed Magnetic beads (22#:PF4)||1||Analysis buffer||1×20mL|
|Pre-Mixed Detection Reagent A||1×120μL||Assay Diluent A||1×12mL|
|Detection Reagent B (PE-SA)||1×120μL||Assay Diluent B||1×12mL|
|Sheath Fluid||1×10mL||Wash Buffer (30 × concentrate)||1×20mL|
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
|Biochimica et Biophysica Acta (BBA) - Proteins & Proteomics||Propofol lowers serum PF4 level and partially corrects hypercoagulopathy in endotoxemic rats PubMed: 20601223|
|Basic Research in Cardiology||Secretome of apoptotic peripheral blood cells (APOSEC) attenuates microvascular obstruction in a porcine closed chest reperfused acute myocardial infarction model: role of platelet aggregation and vasodilation PubMed: 22899170|
|PLoS One||Platelets Recognize Brain-Specific Glycolipid Structures, Respond to Neurovascular Damage and Promote Neuroinflammation PubMed: PMC3608633|
|Diving and Hyperbaric Medicine||Effect of splenectomy on platelet activation and decompression sickness outcome in a rat model of decompression Pubmed:25311322|
|Journal of Functional Foods||A Phellinus baumii–based supplement containing Salvia miltiorrhiza Bunge improves atherothrombotic profiles through endothelial nitric oxide synthase and cyclooxygenase pathwaysin vitro and in vivo science:S1756464616300676|
|Plos one||Aspirin, but Not Tirofiban Displays Protective Effects in Endotoxin Induced Lung Injury pubmed:27583400|
|international journal of molecular sciences||Evidence of the Role of R-Spondin 1 and Its Receptor Lgr4 in the Transmission of Mechanical Stimuli to Biological Signals for Bone Formation. pubmed:28272338|
|Experimental and Therapeutic Medicine||Feasibility of improving platelet‑rich plasma therapy by using chitosan with high platelet activation ability pubmed:28450960|
|Reproductive Toxicology||Aspirin pre-treatment modulates ozone-induced fetal growth restriction and alterations in uterine blood flow in rats Pubmed: 30528429|
|INTERNATIONAL JOURNAL OF CARDIOLOGY||Short and long-term changes in platelet and inflammatory biomarkers after cryoballoon and radiofrequency ablation Pubmed: 30857843|
|Available at SSRN 3335365||Evaluating the Platelet Activation Related to the Degradation of Biomaterials by Scheme of Molecular Markers|
|journal of biotechnology||Newly Identified HNP‐F from Human Neutrophil Peptide 1 Promotes Hemostasis Pubmed: 30927490|
|Data in Brief||Data for short and long-term prothrombotic biomarkers after cryoballoon and radiofrequency ablation|
|journal of thoracic and cardiovascular surgery||Evaluating Platelet Activation Related to the Degradation of Biomaterials Using Molecular Markers Pubmed: 32812629|
|Marine Drugs||The Inhibitory Effect of Protamine on Platelets is Attenuated by Heparin without Inducing Thrombocytopenia in Rodents Pubmed: 31533230|
|Thrombosis Research||Feasibility study of use of rabbit blood to evaluate platelet activation by medical devices Pubmed: 31838449|
|Nat Commun||The C5a/C5a receptor 1 axis controls tissue neovascularization through CXCL4 release from platelets 34099640|
|Vaccine||The effect of ChAdOx1 nCov-19 vaccine on arterial thrombosis development and platelet aggregation in female rats Pubmed:35183388|
|Catalog No.||Related products for research use of Mus musculus (Mouse) Organism species||Applications (RESEARCH USE ONLY!)|
|APA172Mu01||Active Platelet Factor 4 (PF4)||Cell culture; Activity Assays.|
|RPA172Mu01||Recombinant Platelet Factor 4 (PF4)||Positive Control; Immunogen; SDS-PAGE; WB.|
|RPA172Mu02||Recombinant Platelet Factor 4 (PF4)||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAA172Mu01||Polyclonal Antibody to Platelet Factor 4 (PF4)||WB; IHC; ICC; IP.|
|PAA172Mu02||Polyclonal Antibody to Platelet Factor 4 (PF4)||WB; IHC; ICC; IP.|
|LAA172Mu71||Biotin-Linked Polyclonal Antibody to Platelet Factor 4 (PF4)||WB; IHC; ICC.|
|LAA172Mu81||FITC-Linked Polyclonal Antibody to Platelet Factor 4 (PF4)||WB; IHC; ICC; IF.|
|SEA172Mu||ELISA Kit for Platelet Factor 4 (PF4)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|SCA172Mu||CLIA Kit for Platelet Factor 4 (PF4)||Chemiluminescent immunoassay for Antigen Detection.|
|LMA172Mu||Magnetic Luminex Assay Kit for Platelet Factor 4 (PF4) ,etc.||Magnetic Luminex Assay for Antigen Detection.|
|KSA172Mu01||ELISA Kit DIY Materials for Platelet Factor 4 (PF4)||Main materials for "Do It (ELISA Kit) Yourself".|