High Sensitive ELISA Kit for Follicle Stimulating Hormone (FSH)
- Product No.HEA830Ga
- Organism SpeciesChicken (Gallus) Same name, Different species.
- Sample TypeSerum, plasma and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range15.6-1,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 6.3pg/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
For more details, please contact local distributors! US$ 572
For more details, please contact local distributors! US$ 2574
For more details, please contact local distributors! US$ 4862
For more details, please contact local distributors! US$ 40040
For more details, please contact local distributors!
This assay has high sensitivity and excellent specificity for detection of High Sensitive Follicle Stimulating Hormone (FSH).
No significant cross-reactivity or interference between High Sensitive Follicle Stimulating Hormone (FSH) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant High Sensitive Follicle Stimulating Hormone (FSH) and the recovery rates were calculated by comparing the measured value to the expected amount of High Sensitive Follicle Stimulating Hormone (FSH) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive Follicle Stimulating Hormone (FSH) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive Follicle Stimulating Hormone (FSH) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of High Sensitive Follicle Stimulating Hormone (FSH) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
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|Science Alert||Reproductive Toxicity of Tobacco Shisha Smoking on Semen Parameters and Hormones Levels among Adult Egyptian Men SciAlert: source|
|EXCLI Journal||Protective role of Hibiscus sabdariffa calyx extract against streptozotocin induced sperm damage in diabetic rats Excli: Source|
|International Journal of Molecular and Cellular Medicine||Central effects of camphor on GnRH and sexual hormones in male rat Pubmed: 24551777|
|Urologia internationalis||Effects of Tadalafil on Hemorrhagic Cystitis and Testicular Dysfunction Induced by Cyclophosphamide in Rats Pubmed: 24051982|
|Electromagnetic Biology and Medicine||The effects of long-term exposure to a 2450 MHz electromagnetic field on growth and pubertal development in female Wistar rats Informahealthcare: Source|
|Academic Sciences||Piper longum hexane fraction induces infertility by modulation of inflammatory mediators and gonadotropin insufficiency in female rats Ijppsjournal: Source|
|Mol Cell Endocrinol.||Quercetin supplementation restores testicular function and augments germ cell survival in the estrogenized rats Pubmed:24316378|
|Endocrinology||FoxO1 Is a Negative Regulator of FSHβ Gene Expression in Basal and GnRH-Stimulated Conditions in Female Pubmed:24437485|
|Andrologia||Protective effects of Eugenia jambolana extract versus N-acetyl cysteine against cisplatin-induced damage in rat testis Pubmed:24576220|
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|Environmental Research||Perfluorooctane sulfonate effects on the reproductive axis in adult male rats Pubmed:25171141|
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|Reprod Toxicol||Simvastatin decreases steroid production in the H295R cell line and decreases steroids and FSH in female rats PubMed: 26476359|
|The Journal of The Society for Reproduction and Fertility||Sex-specific prenatal stress effects on the rat reproductive axis and adrenal gland structure Pubmed:27026714|
|Biology of Reproduction||Maternal High-Fat Diet Induced Loss of Fetal Oocytes Is Associated with Compromised Follicle Growth in Adult Rat Offspring Pubmed:26962114|
|Andrologia||Dose- and time-dependent effects of Garcinia kola seed extract on sexual behaviour and reproductiveparameters in male Wistar rats. Pubmed:26123866|
|Scientific Reports||Molecular characterization of insulin resistance and glycolytic metabolism in the rat uterus pubmed:27461373|
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|Biology of Reproduction||Maternal High-Fat Diet-Induced Loss of Fetal Oocytes Is Associated with Compromised Follicle Growth in Adult Rat Offspring. pubmed:26962114|
|J Endocrinol.||Impact of uteroplacental insufficiency on postnatal rat male gonad. pubmed:27885054|
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|Environmental Science & Technology||Pyrethroid Insecticide Cypermethrin Accelerates Pubertal Onset in Male Mice via Disrupting Hypothalamic-Pituitary-Gonadal Axis pubmed:28731686|
|Journal of Medicinal Chemistry||Development of New Gonadotropin-Releasing Hormone-Modified Dendrimer Platforms with Direct Antiproliferative and Gonadotropin Releasing Activity pubmed:28958147|
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|Annals of Rheumatic Diseases||FRI0390 Impaired ovarian reserve in patients affected by systemic lupus erythematosus: a case-control study Suppl_2:728.1.abstract|
|Stem Cell Research & Therapy||The role of mesenchymal stem cells in chemotherapy-induced gonadotoxicity Pubmed:30021657|
|Environmental Pollution||Prenatal exposure to polychlorinated biphenyl and umbilical cord hormones and birth outcomes in an island population Pubmed:29525625|
|Frontiers in Molecular Neuroscience||Impact of Triclosan on Female Reproduction through Reducing Thyroid Hormones to Suppress Hypothalamic Kisspeptin Neurons in Mice Pubmed:29403355|
|Biology of Reproduction||Maternal nutrient restriction impairs young adult offspring ovarian signaling resulting in reproductive dysfunction and follicle loss Pubmed:29351580|
|Journal of Ovarian Research||DHEA-induced ovarian hyperfibrosis is mediated by TGF-β signaling pathway Pubmed:29321035|
|Medical Science Monitor||Eicosapentaenoic Acid Improves Polycystic Ovary Syndrome in Rats via Sterol Regulatory Element-Binding Protein 1 (SREBP-1)/Toll-Like Receptor 4 (TLR4) … Pubmed:29627845|
|Human & Experimental Toxicology||Palladium nanoparticle effects on endocrine reproductive system of female rats Pubmed:29405765|
|A Pex13 knockout in germ cells induces a spermatogenic arrest volltexte:2018/8921/|
|Biomedicine & Pharmacotherapy||Crocetin attenuates DHT-induced polycystic ovary syndrome in mice via revising kisspeptin neurons Doi: 10.1016/j.biopha.2018.08.135|
|Stem Cells Translational Medicine||Protective Effects of Uncultured Adipose‐Derived Stromal Vascular Fraction on Testicular Injury Induced by Torsion‐Detorsion in Rats Pubmed: 30569668|
|Journal of Endocrinology||Uterine progesterone signaling is a target for metformin therapy in polycystic ovary syndrome Pubmed: 29535146|
|Experimental and Therapeutic Medicine||Tanshinone IIA attenuates estradiol‑induced polycystic ovarian syndrome in mice by ameliorating FSHR expression in the ovary|
|Biomedicine & Pharmacotherapy||Protective properties of glycogen synthase kinase-3 inhibition against doxorubicin-induced oxidative damage to mouse ovarian reserve|
|Biology of Reproduction||G-CSF-mobilized Peripheral Blood Mononuclear Cells Combined with Platelet-rich Plasma Accelerates Restoration of Ovarian Function in Cyclophosphamide … Pubmed: 31034039|
|International Journal of Reproductive Biomedicine||The therapeutic potential of amifostine on cyclophosphamide-induced testicular dysfunction in rats: An experimental study.|
|Reproductive Biology and Endocrinology||Menstrual blood derived mesenchymal stem cells combined with Bushen Tiaochong recipe improved chemotherapy-induced premature ovarian failure in … Pubmed: 31311554|
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|Catalog No.||Related products for research use of Chicken (Gallus) Organism species||Applications (RESEARCH USE ONLY!)|
|HEA830Ga||High Sensitive ELISA Kit for Follicle Stimulating Hormone (FSH)||Enzyme-linked immunosorbent assay for Antigen Detection.|
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