High Sensitive ELISA Kit for Cortisol (Cor)

Hydrocortisone

Specificity

This assay has high sensitivity and excellent specificity for detection of High Sensitive Cortisol (Cor).
No significant cross-reactivity or interference between High Sensitive Cortisol (Cor) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of High Sensitive Cortisol (Cor) and the recovery rates were calculated by comparing the measured value to the expected amount of High Sensitive Cortisol (Cor) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 79-104 92
EDTA plasma(n=5) 95-103 99
heparin plasma(n=5) 80-103 90

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive Cortisol (Cor) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive Cortisol (Cor) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of High Sensitive Cortisol (Cor) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 86-101% 80-104% 95-103% 93-101%
EDTA plasma(n=5) 96-105% 99-105% 98-105% 83-90%
heparin plasma(n=5) 84-103% 87-95% 80-92% 92-103%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Research in Veterinary Science Use of infrared thermography to assess the influence of high environmental temperature on rabbits ScienceDirect: S0034528813001380
British Journal of Nutrition Identification of sociodemographic and clinical factors associated with the levels of human b-defensin-1 and human b-defensin-2 in the human milk of Han Chinese Pubmed:24124699
Theriogenology Daily exposure to summer circadian cycles affects spermatogenesis, but not fertility in an in vivo rabbit model Pubmed:25442388
Animal Production Science Effect of high temperatures on breeding rabbit behaviour Publish:Source
JBCD Assessment of cortisol as salivary psychological stress marker in relation to temporomandibular disorders among a sample of dental students Index.Php: Jbcd
International Journal of Immunopathology & Pharmacology Sea buckthorn (Hippophae rhamnoides L.) oil protects against chronic stress-induced inhibitory function of natural killer cells in rats PubMed: 26684638
IET Micro & Nano Letters Surface plasmon resonance immunoassay for cortisol determination with a self-assembling denaturalised bovine serum albumin layer on surface plasmon resonance chip xpls:7393900
Optoelectronics Letters Fabrication and characterization of SPR chips with the modified bovine serum albumin article:10.1007
Journal of Dairy Science Effect of thermal stress on physiological, hormonal and haematological parameters in Tharparkar and Karan Fries calves publication:306322503
Acta Veterinaria Hungarica Relationship between the indexes of insulin resistance and metabolic status in dairy cows during early lactation acve-2017-0006
BMC Veterinary Research Hypothalamus-pituitary-adrenal axis involves in anti-viral ability through regulation of immune response in piglets infected by highly pathogenic porcine … Pubmed:29540178
Indian Journal of Animal Sciences Endurance exercise causes adverse changes in some hematological and physio-biochemical indices in ponies under high altitude stress condition :
Biological Rhythm Research Effects of feeding frequency during lactation in primiparous sows on reproduction performance and diurnal rhythm of endocrine profiles at the weaning-to-ovulation …
International Journal of Current Microbiology and Applied Sciences Physiological Performance of Crossbred Cattle Calves (Karan Fries) under Different Housing Conditions during Different Seasons
Journal of Animal & Plant Sciences A study on certain blood biomarkers of pregnant Cholistani cows.
Animals The Use of Infrared Thermography (IRT) as Stress Indicator in Horses Trained for Endurance: A Pilot Study Pubmed: 30866503
Medicine Saliva cortisol, melatonin levels and circadian rhythm alterations in Chinese primary school children with dyslexia Pubmed: 32028434
MICROBIAL PATHOGENESIS Assessment of certain biomarkers for predicting survival in response to treatment in dogs naturally infected with canine parvovirus Pubmed: 32926999
AQUACULTURE RESEARCH Effects of dietary nucleotides on growth, survival and metabolic response in whiteleg shrimp, Litopenaeus vannamei against ammonia stress condition
AQUACULTURE RESEARCH Physiological responses to salinity stress in the Managua Cichlid, Cichlasoma managuense
Anti-stress activity of some plants extracts of the North Caucasus flora
Animal Effect of alfalfa hay and starter feed supplementation on caecal microbiota and fermentation, growth, and health of yak calves 33610512
Parasit Vectors Exploring alterations in hematological and biochemical parameters, enzyme activities and serum cortisol in Besnoitia besnoiti naturally infected dairy cattle 33722267
J Am Heart Assoc Post©\Cardiac Arrest Hydrocortisone Use Ameliorates Cardiac Mitochondrial Injury in a Male Rat Model of Ventricular Fibrillation Cardiac Arrest 33960200
Animals The Protective Role of Alpha-Ketoglutaric Acid on the Growth and Bone Development of Experimentally Induced Perinatal Growth-Retarded Piglets 33435211
Preventive Veterinary Medicine Stress response after race and endurance training sessions and competitions in Arabian horses 33497894
Current Psychology Associations of Chinese social face with cortisol level and glucocorticoid receptor gene
Orexin-A/OX1 Receptor Regulates Autophagy, Mitochondrial Autophagy, and Cortisol Secretion in Human Adrenocortical Cells Through the mTORC1/P70s6k1 …
Catalog No. Related products for research use of Pan-species (General) Organism species Applications (RESEARCH USE ONLY!)
CPA462Ge11 BSA Conjugated Cortisol (Cor) Immunogen; SDS-PAGE; WB.
CPA462Ge21 OVA Conjugated Cortisol (Cor) Immunogen; SDS-PAGE; WB.
PAA462Ge01 Polyclonal Antibody to Cortisol (Cor) ELISA, CLIA. / IHC-Fr, ICC, IP (predicted).
LAA462Ge81 FITC-Linked Polyclonal Antibody to Cortisol (Cor) WB; IHC; ICC; IF.
LAA462Ge71 Biotin-Linked Polyclonal Antibody to Cortisol (Cor) WB; IHC; ICC.
MAA462Ge21 Monoclonal Antibody to Cortisol (Cor) ELISA, CLIA. / IHC-Fr, ICC, IP (predicted).
MAA462Ge22 Monoclonal Antibody to Cortisol (Cor) ELISA, CLIA. / IHC-Fr, ICC, IP (predicted).
MEA462Ge Mini Samples ELISA Kit for Cortisol (Cor) Enzyme-linked immunosorbent assay for Antigen Detection.
IEA462Ge Instant ELISA Kit for Cortisol (Cor) Enzyme-linked immunosorbent assay for Antigen Detection.
HEA462Ge High Sensitive ELISA Kit for Cortisol (Cor) Enzyme-linked immunosorbent assay for Antigen Detection.
CEA462Ge ELISA Kit for Cortisol (Cor) Enzyme-linked immunosorbent assay for Antigen Detection.
CCA462Ge CLIA Kit for Cortisol (Cor) Chemiluminescent immunoassay for Antigen Detection.
LMA462Ge Multiplex Assay Kit for Cortisol (Cor) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSA462Ge11 ELISA Kit DIY Materials for Cortisol (Cor) Main materials for "Do It (ELISA Kit) Yourself".