High Sensitive ELISA Kit for Luteinizing Hormone (LH)
ICSH; Lutropin; Interstitial Cell Stimulating Hormone
- Product No.HEA441Ga
- Organism SpeciesChicken (Gallus) Same name, Different species.
- Sample TypeSerum, plasma and other biological fluids
- Test MethodCompetitive Inhibition
- Assay Length3h
- Detection Range123.5-10,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 58.5pg/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
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For more details, please contact local distributors! US$ 52640
For more details, please contact local distributors!
This assay has high sensitivity and excellent specificity for detection of High Sensitive Luteinizing Hormone (LH).
No significant cross-reactivity or interference between High Sensitive Luteinizing Hormone (LH) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant High Sensitive Luteinizing Hormone (LH) and the recovery rates were calculated by comparing the measured value to the expected amount of High Sensitive Luteinizing Hormone (LH) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive Luteinizing Hormone (LH) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive Luteinizing Hormone (LH) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of High Sensitive Luteinizing Hormone (LH) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.
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|International Journal of Clinical and Experimental Medicine||Studies of the pharmacology of 17α-ethynyl-androst-5-ene-3β,7β,17β-triol, a synthetic anti-inflammatory androstene Ijcem: IJCEM1102004|
|Biochemical Journal||PPARγ co-activator-1α co-activates steroidogenic factor 1 to stimulate the synthesis of luteinizing hormone and aldosterone PubMed: 21108604|
|Molecular and Cellular Endocrinology||Effects of genistein and estrogen receptor subtype-specific agonists in ArKO mice following different administration routes ScienceDirect: S0303720709004043|
|Asian Journal of Andrology||Brief maternal exposure of rats to the xenobiotics dibutyl phthalate or diethylstilbestrol alters adult-type Leydig cell development in male offspring PubMed: 23314658|
|EXCLI Journal||Protective role of Hibiscus sabdariffa calyx extract against streptozotocin induced sperm damage in diabetic rats Excli: Source|
|International Journal of Molecular and Cellular Medicine||Central effects of camphor on GnRH and sexual hormones in male rat Pubmed: 24551777|
|Reproductive Toxicology||Inhibitory effects of mitotane on viability and secretory activity in mouse gonadotroph cell lines Pubmed: 24486453|
|Urologia internationalis||Effects of Tadalafil on Hemorrhagic Cystitis and Testicular Dysfunction Induced by Cyclophosphamide in Rats Pubmed: 24051982|
|Journal of Materials Chemistry B||Nanocomposites of bio-based hyperbranched polyurethane/funtionalized MWCNT as non-immunogenic, osteoconductive, biodegradable and biocompatible scaffolds in bone tissue engineering Rsc: Source|
|Electromagnetic Biology and Medicine||The effects of long-term exposure to a 2450 MHz electromagnetic field on growth and pubertal development in female Wistar rats Informahealthcare: Source|
|Academic Sciences||Piper longum hexane fraction induces infertility by modulation of inflammatory mediators and gonadotropin insufficiency in female rats Ijppsjournal: Source|
|Biochemical and Biophysical Research Communications||Critical function of RA-GEF-2/Rapgef6, a guanine nucleotide exchange factor for Rap1, in mouse spermatogenesis ScienceDirect: S0006291X14001843|
|Reproductive Toxicology||Carbamazepine-exposure during gestation and lactation affects pubertal onset and spermatic parameters in male pubertal offspring Pubmed:24126190|
|Mol Cell Endocrinol.||Quercetin supplementation restores testicular function and augments germ cell survival in the estrogenized rats Pubmed:24316378|
|Andrologia||Protective effects of Eugenia jambolana extract versus N-acetyl cysteine against cisplatin-induced damage in rat testis Pubmed:24576220|
|Environmental Research||Perfluorooctane sulfonate effects on the reproductive axis in adult male rats Pubmed:25171141|
|Genet Mol Biol.||Targeted disruption of the mouse testis-enriched gene Znf230 does not affect spermatogenesis or fertility Pubmed:25505846|
|Turkish Journal of Urology||Apoptosis in testicular tissue of rats after vasectomy: evaluation of eNOS, iNOS immunoreactivities and the effects of ozone therapy Turkishjournalofurology:Source|
|Andrologia||Protective effects of Eugenia jambolana extract versus N‐acetyl cysteine against cisplatin‐induced damage in rat testis PubMed: 24576220|
|European Journal of Pharmacology||The effect of R-(-)-deprenyl administration on reproductive parameters of rat males PubMed: 25725114|
|Andrologia||Dose‐and time‐dependent effects of Garcinia kola seed extract on sexual behaviour and reproductive parameters in male Wistar rats PubMed: 26123866|
|Reprod Toxicol||Simvastatin decreases steroid production in the H295R cell line and decreases steroids and FSH in female rats PubMed: 26476359|
|Biology of Reproduction||Maternal High-Fat Diet Induced Loss of Fetal Oocytes Is Associated with Compromised Follicle Growth in Adult Rat Offspring Pubmed:26962114|
|Andrologia||Dose- and time-dependent effects of Garcinia kola seed extract on sexual behaviour and reproductiveparameters in male Wistar rats. Pubmed:26123866|
|Int J Mol Sci.||Overexpression of PRL7D1 in Leydig Cells Causes Male Reproductive Dysfunction in Mice Pubmed:26771609|
|PLoS Genet.||A Jacob/Nsmf Gene Knockout Results in Hippocampal Dysplasia and Impaired BDNF Signaling in Dendritogenesis Pubmed:26977770|
|Scientific Reports||Molecular characterization of insulin resistance and glycolytic metabolism in the rat uterus pubmed:27461373|
|33||Metformin Ameliorates Uterine Defects in a Rat Model of Polycystic Ovary Syndrome. pubmed:28336389|
|environmental pollution||Environmental exposure to polycyclic aromatic hydrocarbons (PAHs): The correlation with and impact on reproductive hormones in umbilical cord serum. pubmed:27838061|
|Toxicological Sciences||Exposure of pregnant mice to perfluorobutanesulfonate causes hypothyroxinemia and developmental abnormalities in female offspring pubmed:27803384|
|Andrologia||Effects of treatment with Hypoxis hemerocallidea extract on sexual behaviour and reproductiveparameters in male rats. pubmed:28000943|
|Biology of Reproduction||Maternal High-Fat Diet-Induced Loss of Fetal Oocytes Is Associated with Compromised Follicle Growth in Adult Rat Offspring. pubmed:26962114|
|J Endocrinol.||Impact of uteroplacental insufficiency on postnatal rat male gonad. pubmed:27885054|
|Int J Pharm.||New gonadotropin-releasing hormone glycolipids with direct antiproliferative activity and gonadotropin-releasing potency. pubmed:28232269|
|Environmental Science & Technology||Pyrethroid Insecticide Cypermethrin Accelerates Pubertal Onset in Male Mice via Disrupting Hypothalamic-Pituitary-Gonadal Axis pubmed:28731686|
|Journal of Medicinal Chemistry||Development of New Gonadotropin-Releasing Hormone-Modified Dendrimer Platforms with Direct Antiproliferative and Gonadotropin Releasing Activity pubmed:28958147|
|Society of Toxicology||Pyrethroid Insecticide Cypermethrin Modulates Gonadotropin Synthesis via Calcium Homeostasis and ERK1/2 Signaling in LbT2 Mouse Pituitary Cells pubmed:29149324|
|Reproductive Toxicology||Vitamin D deficiency impairs testicular development and spermatogenesis in mice pubmed:28655646|
|The Journal of Biological Chemistry.||Lysophosphatidic acid acyltransferase 3 tunes the membrane status of germ cells by incorporating docosahexaenoic acid during spermatogenesis. pubmed:28578315|
|The Journal of Toxicological Sciences||High sensitivity of testicular function to titanium nanoparticles pubmed:28496042|
|Journal of Orthopaedic Research||The Combination of icariin and constrained dynamic loading stimulation attenuates bone loss in ovariectomy‐induced osteoporotic mice pubmed:29058779|
|Anticancer Research||Transdermal Delivery of Luteinizing Hormone-releasing Hormone with Chitosan Microneedles: A Promising Tool for Androgen Deprivation Therapy pubmed:29187457|
|DARU Journal of Pharmaceutical Sciences||Beneficial effects of Heqi san on rat model of polycystic ovary syndrome through the PI3K/AKT pathway pubmed:29020999|
|Reproduction.||Effect of controlled ovarian hyperstimulation on puberty and estrus in mice offspring pubmed:28687593|
|Archives of Toxicology||Atorvastatin decreases steroid production in H295R cells and in major endocrine tissues of male rats Pubmed:29574565|
|FASEB Journal||Vasorin: a newly identified regulator of ovarian folliculogenesis Pubmed:29259033|
|Stem Cell Research & Therapy||The role of mesenchymal stem cells in chemotherapy-induced gonadotoxicity Pubmed:30021657|
|Environmental Pollution||Prenatal exposure to polychlorinated biphenyl and umbilical cord hormones and birth outcomes in an island population Pubmed:29525625|
|Toxicological Sciences||Impact of Perfluorooctane Sulfonate on Reproductive Ability of Female Mice through Suppression of Estrogen Receptor a-Activated Kisspeptin Neurons 10.1093:toxsci|
|Frontiers in Molecular Neuroscience||Impact of Triclosan on Female Reproduction through Reducing Thyroid Hormones to Suppress Hypothalamic Kisspeptin Neurons in Mice Pubmed:29403355|
|Biology of Reproduction||Maternal nutrient restriction impairs young adult offspring ovarian signaling resulting in reproductive dysfunction and follicle loss Pubmed:29351580|
|Journal of Ovarian Research||DHEA-induced ovarian hyperfibrosis is mediated by TGF-β signaling pathway Pubmed:29321035|
|Medical Science Monitor||Eicosapentaenoic Acid Improves Polycystic Ovary Syndrome in Rats via Sterol Regulatory Element-Binding Protein 1 (SREBP-1)/Toll-Like Receptor 4 (TLR4) … Pubmed:29627845|
|Human & Experimental Toxicology||Palladium nanoparticle effects on endocrine reproductive system of female rats Pubmed:29405765|
|A Pex13 knockout in germ cells induces a spermatogenic arrest volltexte:2018/8921/|
|Biomedicine & Pharmacotherapy||Crocetin attenuates DHT-induced polycystic ovary syndrome in mice via revising kisspeptin neurons Doi: 10.1016/j.biopha.2018.08.135|
|Biological Rhythm Research||Effects of feeding frequency during lactation in primiparous sows on reproduction performance and diurnal rhythm of endocrine profiles at the weaning-to-ovulation …|
|Stem Cells Translational Medicine||Protective Effects of Uncultured Adipose‐Derived Stromal Vascular Fraction on Testicular Injury Induced by Torsion‐Detorsion in Rats Pubmed: 30569668|
|Journal of Endocrinology||Uterine progesterone signaling is a target for metformin therapy in polycystic ovary syndrome Pubmed: 29535146|
|Experimental and Therapeutic Medicine||Tanshinone IIA attenuates estradiol‑induced polycystic ovarian syndrome in mice by ameliorating FSHR expression in the ovary|
|Theriogenology||Organic Zn and Cu interaction impact on sexual behaviour, semen characteristics, hormones and spermatozoal gene expression in bucks (Capra hircus)|
|International Journal of Reproductive Biomedicine||The therapeutic potential of amifostine on cyclophosphamide-induced testicular dysfunction in rats: An experimental study.|
|Andrologia||Sperm parameters quality and reproductive effects of methanolic extract of Alchornea cordifolia leaves on senescent male rats Pubmed: 31353623|
|Catalog No.||Related products for research use of Chicken (Gallus) Organism species||Applications (RESEARCH USE ONLY!)|
|HEA441Ga||High Sensitive ELISA Kit for Luteinizing Hormone (LH)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|CEA441Ga||ELISA Kit for Luteinizing Hormone (LH)||Enzyme-linked immunosorbent assay for Antigen Detection.|