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Instruction Manual
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Eukaryotic Angiotensinogen (AGT)
ANHU; SERPINA8; Angiotensin; Renin Substrate; Serpin Peptidase Inhibitor,Clade A,Member 8(Alpha-1 Antiproteinase,Antitrypsin)
- Product No.EPA797Ra61
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- SourceEukaryotic expression
- Host293F cell
- Endotoxin Level<1.0EU per 1ug (determined by the LAL method)
- Subcellular LocationSecreted
- Predicted Molecular Mass51.2kDa
- Accurate Molecular Mass60kDa(Analysis of differences refer to the manual)
- Residues & TagsAsp25~Val477 with N-terminal His Tag
- Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 5% Trehalose and Proclin300.
- Traits Freeze-dried powder
- Purity> 97%
- Isoelectric Point5.3
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Applications
Positive Control; Immunogen; SDS-PAGE; WB.
If bio-activity of the protein is needed, please check active protein. - DownloadInstruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
- FOB
US$ 166
US$ 415
US$ 830
US$ 2490
US$ 6225
For more details, please contact local distributors!
SEQUENCE
USAGE
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
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BCA Protein Quantification Kit
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Protein Labeling Customized Service
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Molecular Mass Marker for Protein
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Monoclonal Antibody Customized Service
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Polyclonal Antibody Customized Service
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Protein Activity Test Experiment Service
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Immunoprecipitation (IP) Experiment Service
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Electrophoretic Mobility Shift Assay (EMSA) Experiment Service
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Buffer
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Lentivirus Packaging Experiment Service
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Adenovirus Packaging Experiment Service
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Real Time PCR Experimental Service
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Spike RBD Protein (S-RBD)
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Protein G
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Protein A
| Magazine | Citations |
| Transplant International | Elevated urinary angiotensinogen a marker of intrarenal renin angiotensin system in hypertensive renal transplant recipients: does it play a role in development of proteinuria in hypertensive renal transplant patients? Wiley: source |
| Amyloid | The relation between urinary angiotensinogen and proteinuria in renal AA amyloidosis patients PubMed: 22320202 |
| Pediatric Mephrology | Urinary angiotensinogen as a marker of intrarenal angiotensin II activity in adolescents with primary hypertension PubMed: PMC3661928 |
| Acta Paediatrica | Urinary angiotensinogen as a novel marker of obstructive nephropathy in children Pubmed: 23772991 |
| Journal of the Renin-Angiotensin-Aldosterone System | Association between urinary angiotensinogen, hypertension and proteinuria in pregnant women with preeclampsia Sagepub: Source |
| Pediatric Nephrology | MP707 CHARACTERISTICS AND OUTCOMES OF CHILDREN WITH AUTOSOMAL RECESSIVE POLYCYSTIC KIDNEY DISEASE COHORT FROM THE ESPN/ERA-EDTA REGISTRY Oxfordjournals:Source |
| journal of human hypertension | Relationship between blood pressure var PubMed: 26223347 |
| PLoS One | Higher Serum Angiotensinogen Is an Indicator of IgA Vasculitis with Nephritis Revealed by Comparative Proteomes Analysis PubMed: 26098644 |
| MKA | Korelasi pengukuran antropometri dengan tekanan darah dan angiotensinogen plasma pada dewasa Index.Php: Art |
| Journal of Human Hypertension | Relationship between blood pressure variability and renal activity of the renin-angiotensin system. pubmed:26223347 |
| Acta Pharmacol Sin. | Pharmacological evidence: a new therapeutic approach to the treatment of chronic heart failure through SUR2B/Kir6.1 channel in endothelial cells. pubmed:27890915 |
| Journal of Chromatography A | Application of a new procedure for liquid chromatography/mass spectrometry profiling of plasma amino acid-related metabolites and untargeted shotgun proteomics to identify mechanisms and biomarkers of calcific aortic stenosis S0021967317311858 |
| Life Sciences | Pyrrolidine dithiocarbamate inhibits mouse acute kidney injury induced by diclofenac by targeting oxidative damage, cytokines and NF-κB activity Pubmed:30036488 |
| American Journal of Physiology-Renal Physiology | Role of (Pro) Renin Receptor in Albumin Overload-Induced Nephropathy in Rats Pubmed:29846109 |
| Methods in Molecular Biology | Quantitation of Total and Free Thiol Angiotensinogen as a Prognostic Marker for Preeclampsia Pubmed: 31069778 |
| J Transl Med | Multi-omic signatures of atherogenic dyslipidaemia: pre-clinical target identification and validation in humans 33407555 |
| Hypertens Res | Association of Ang-(1¨C7) and des-Arg 9 BK as new biomarkers of obesity and cardiometabolic risk factors in adolescents 33568792 |
| Physiol Rep | (Pro) renin receptor antagonist PRO20 attenuates nephrectomy©\induced nephropathy in rats via inhibition of intrarenal RAS and Wnt/¦Â©\catenin signaling 34057312 |
| Cancer Cell Int | A novel strategy to identify candidate diagnostic and prognostic biomarkers for gastric cancer 34215253 |