ELISA Kit for Phosphatidylinositol (PI)



This assay has high sensitivity and excellent specificity for detection of Phosphatidylinositol (PI).
No significant cross-reactivity or interference between Phosphatidylinositol (PI) and analogues was observed.


Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Phosphatidylinositol (PI) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Phosphatidylinositol (PI) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%


The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1 Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
Reagent Diluent 1×300µL Stop Solution 1×6mL
TMB Substrate 1×9mL Instruction manual 1
Wash Buffer (30 × concentrate) 1×20mL

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.



Catalog No. Related products for research use of Pan-species (General) Organism species Applications (RESEARCH USE ONLY!)
CPG853Ge11 BSA Conjugated Phosphatidylinositol (PI) Immunogen; SDS-PAGE; WB.
CPG853Ge21 OVA Conjugated Phosphatidylinositol (PI) Immunogen; SDS-PAGE; WB.
PAG853Ge01 Polyclonal Antibody to Phosphatidylinositol (PI) WB; IHC; ICC; IP.
CEG853Ge ELISA Kit for Phosphatidylinositol (PI) Enzyme-linked immunosorbent assay for Antigen Detection.
KSG853Ge11 ELISA Kit DIY Materials for Phosphatidylinositol (PI) Main materials for "Do It (ELISA Kit) Yourself".