ELISA Kit for Oxytocin (OT)
- Product No.CEB052Ge
- Organism SpeciesPan-species (General) Same name, Different species.
- Sample TypeSerum, plasma and other biological fluids
- Test MethodCompetitive Inhibition
- Assay Length2h
- Detection Range12.35-1,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 5.51pg/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
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This assay has high sensitivity and excellent specificity for detection of Oxytocin (OT).
No significant cross-reactivity or interference between Oxytocin (OT) and analogues was observed.
Matrices listed below were spiked with certain level of Oxytocin (OT) and the recovery rates were calculated by comparing the measured value to the expected amount of Oxytocin (OT) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Oxytocin (OT) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Oxytocin (OT) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Oxytocin (OT) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|Reagent Diluent||1×300µL||Stop Solution||1×6mL|
|TMB Substrate||1×9mL||Instruction manual||1|
|Wash Buffer (30 × concentrate)||1×20mL|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.
|J Psychiatr Res.?||Sex-specific associations between plasma oxytocin levels and schizotypal personality features in healthy individuals Pubmed:24411593|
|Fertility and Sterility||Administration of atosiban in patients with endometriosis undergoing frozenâthawed embryo transfer: a prospective, randomized study Pubmed:27143518|
|Progress in neuro-psychopharmacology & biological psychiatry||Increased oxytocin levels among abstinent heroin addicts: Association with aggressiveness, psychiatric symptoms and perceived childhood neglect. pubmed:28093220|
|International Journal of Clinical and Experimental Medicine||Can hypoxia enhance sexual arousal?-Molecular-biological analysis of the hypothalamus in male rats placed with oestrous female rats under hypoxic … files:ijcem0031358.pdf|
|Fertility and Sterility||Administration of atosiban in patients with endometriosis undergoing frozen–thawed embryo transfer: a prospective, randomized study pubmed:27143518|
|BMC Med.||Intranasal oxytocin reduces provoked symptoms in female patients with posttraumatic stress disorder despite exerting sympathomimetic and positive chronotropic effects in a randomized controlled trial. pubmed:28209155|
|Psychiatry Research||Plasma oxytocin levels in major depressive and bipolar II disorders pubmed:28865715|
|BMC Medicine||Intranasal oxytocin reduces provoked symptoms in female patients with posttraumatic stress disorder despite exerting sympathomimetic and positive chronotropic effects in a randomized controlled trial 10.1186:s12916-017-0801-0|
|EUROPEAN ADDICTION RESEARCH||The Association between the Level of Plasma Oxytocin and Craving among Former Heroin Users Pubmed:29902803|
|Catalog No.||Related products for research use of Pan-species (General) Organism species||Applications (RESEARCH USE ONLY!)|
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|PAB052Ge01||Polyclonal Antibody to Oxytocin (OT)||WB; IHC; ICC; IP.|
|HEB052Ge||High Sensitive ELISA Kit for Oxytocin (OT)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|CEB052Ge||ELISA Kit for Oxytocin (OT)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|KSB052Ge11||ELISA Kit DIY Materials for Oxytocin (OT)||Main materials for "Do It (ELISA Kit) Yourself".|