ELISA Kit for Noradrenaline (NE)

NA; NAd; Norepinephrine; Norepi

Specificity

This assay has high sensitivity and excellent specificity for detection of Noradrenaline (NE).
No significant cross-reactivity or interference between Noradrenaline (NE) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of Noradrenaline (NE) and the recovery rates were calculated by comparing the measured value to the expected amount of Noradrenaline (NE) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 85-102 95
EDTA plasma(n=5) 97-105 101
heparin plasma(n=5) 99-105 102

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Noradrenaline (NE) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Noradrenaline (NE) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Noradrenaline (NE) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 94-105% 88-96% 95-104% 78-89%
EDTA plasma(n=5) 87-101% 89-104% 81-102% 96-103%
heparin plasma(n=5) 96-104% 88-96% 80-98% 99-105%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.

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Magazine Citations
Renal Failure Circulating Levels of Renalase, Norepinephrine, and Dopamine in Dialysis Patients Pubmed: 23530612
Acta Physiologica Apelin-13 and APJ in paraventricular nucleus contribute to hypertension via sympathetic activation and vasopressin release in spontaneously hypertensive rats Pubmed:24995933
Physiological Research Renal Sympathetic Denervation Improves Cardiac Dysfunction PubMed: 25804096
J Cardiovasc Pharmacol Ther Erythropoietin Reverses Sepsis-Induced Vasoplegia to Norepinephrine Through Preservation of 伪1D-Adrenoceptor mRNA Expression and Inhibition of GRK2-Mediated Desensitization in Mouse Aorta. PubMed: 26025460
Neuropsychiatr Dis Treat Catha edulis chewing effects on treatment of paranoid schizophrenic patients PubMed: 25926735
Journal of Caediovascular Pharmacology and Therapeutics Erythropoietin Reverses Sepsis-Induced Vasoplegia to Norepinephrine Through Preservation of α1D-Adrenoceptor mRNA Expression and Inhibition of GRK2-Mediated Desensitization in Mouse Aorta content:21
Neuropharmacology Relaxin in paraventricular nucleus contributes to sympathetic overdrive and hypertension via PI3K-Akt pathway Pubmed:26746861
Microchemical Journal Determination of 17β-estradiol and noradrenaline in dog serum using surface-enhanced Raman spectroscopy and random Forest science:S0026265X16300388
Scientific Reports Reduced lipolysis response to adipose afferent reflex involved in impaired activation of adrenoceptor-cAMP-PKA-hormone sensitive lipase pathway in obesity pubmed:27694818
PLoS One. Artepillin C, a Typical Brazilian Propolis-Derived Component, Induces Brown-Like Adipocyte Formation in C3H10T1/2 Cells, Primary Inguinal White Adipose Tissue-Derived Adipocytes, and Mice. pubmed:27598888
Cellular Physiology and Biochemistry Renal Denervation Attenuates Multi-Organ Fibrosis and Improves Vascular Remodeling in Rats with Transverse Aortic Constriction Induced Cardiomyopathy pubmed:27889753
European Journal of Trauma and Emergency Xuebijing injection treatment inhibits vasopermeability and reduces fluid requirements in acanine burn model. pubmed:28070608
Acta Physiologica c-Jun N-terminal Kinase mediates prostaglandin-induced sympathoexcitation in rats with chronic heart failure by reducing GAD1 and GABRA1 expression pubmed:27439062
Nutrition Ginkgo biloba extract alleviates oxidative stress and some neurotransmitters changes induced by aluminum chloride in rats S0899900716302350
Auris Nasus Larynx. Stress hormonal changes in the brain and plasma after acute noise exposure in mice. pubmed:27496010
Oncotarget Effects of renal denervation on monocrotaline induced pulmonary remodeling pubmed:28187460
JOURNAL OF APPLIED PHYSIOLOGY  Renal sympathetic denervation attenuates hypertension and vascular remodeling in renovascular hypertensive rats pubmed:27742806
Nutrition Ginkgo Biloba Extract Alleviates Oxidative Stress and Some Neurotransmitters Changes Induced by Aluminum Chloride in Rats pubmed:28241996
Acta Physiologica Beneficial Effects of Renal Denervation on Cardiac Angiogenesis in Rats with Prolonged Pressure Overload pubmed:27575955
Proceedings of the National Academy of Sciences of the United States of America (PNAS) cAMP-inducible coactivator CRTC3 attenuates brown adipose tissue thermogenesis Pubmed:29784793
Toxicology Reports Neuro-and nephroprotective effect of grape seed proanthocyanidin extract against carboplatin and thalidomide through modulation of inflammation, tumor … Pubmed:29854627
International Journal of Nursing Practice Non‐pharmacological interventions during childbirth for pain relief, anxiety, and neuroendocrine stress parameters: A randomized controlled trial Pubmed:29512230
Acta Astronautica A novel sympathetic neuronal GABA ergic signaling system regulates NE release to prevent ventricular arrhythmias after acute myocardial infarction
Journal of Taibah University for Science Neurotoxicity and inflammation induced by individual and combined exposure to iron oxide nanoparticles and silver nanoparticles
Journal of the American College of Cardiology Noninvasive Stereotactic Radiotherapy for Renal Denervation in a Swine Model Pubmed: 31558254
American Journal of Physiology-Heart and Circulatory Physiology Exacerbated pressor and sympatho-excitatory effects of central Elabela in spontaneously hypertensive rats Pubmed: 31834836
European Journal of Pharmacology Resiniferatoxin reduces ventricular arrhythmias in heart failure via selectively blunting cardiac sympathetic afferent projection into spinal cord in rats Pubmed: 31811858
A porcine model for studying the cardiovascular consequences of high‐thoracic spinal cord injury Pubmed: 31876952
The American Journal of Pathology Sympathetic Nerves Positively Regulate Eosinophil-Driven Allergic Conjunctivitis via α1-Adrenergic Receptor Signaling Pubmed: 32194050
pharmaceutical biology Role of melatonin in intestinal mucosal injury induced by restraint stress in mice Pubmed: 32298156
Nature Feeding induces cholesterol biosynthesis via the mTORC1–USP20–HMGCR axis Pubmed: 33177714
European Journal of Medical Research Laparoscopic-based perivascular renal sympathetic nerve denervation: a feasibility study in a porcine model Pubmed: 32552871
Scientific Reports Baroreceptor denervation reduces inflammatory status but worsens cardiovascular collapse during systemic inflammation Pubmed: 32332859
bioRxiv Baroreceptor denervation reduces inflammatory status and worsens cardiovascular collapse during systemic inflammation
Blockade of adrenergic β‐receptor activation through local delivery of propranolol from a 3D collagen/polyvinyl alcohol/hydroxyapatite scaffold promotes bone repair …
Cogent Medicine Fractionation and bioassay-guided isolation of antihypertensive components of senecio serratuloides
PHARMACOLOGICAL RESEARCH Piezo2 channel in nodose ganglia neurons is essential in controlling hypertension in a pathway regulated directly by Nedd4-2 33352230
ACTA PHARMACOLOGICA SINICA The vascular endothelial growth factor trap aflibercept induces vascular dysfunction and hypertension via attenuation of eNOS/NO signaling in mice 33303990
Mol Metab Adipose tissue lipolysis is regulated by PAQR11 via altering protein stability of phosphodiesterase 4D 33549845
International Journal of?Nanomedicine Water-Soluble Carbon Dots in Cigarette Mainstream Smoke: Their Properties and the Behavioural, Neuroendocrinological, and Neurotransmitter Changes They ¡­ 33762821
Int J Hypertens The Effects of Renal Nerve Denervation on Blood Pressure and Target Organs in Different Hypertensive Rat Models 33884205
Kidney Blood Press Res Renal interstitial fibrosis is reduced in high-fat diet-induced obese pigs following renal denervation from the intima and adventitia of the renal artery 34852339
Hypertension Expression of LHCGR (Luteinizing Hormone/Chorionic Gonadotrophin Receptor) in Pheochromocytomas Unveils an Endocrine Mechanism Connecting Pregnancy … Pubmed:35189708
Exp Eye Res Autonomic nervous system receptor-mediated regulation of mast cell degranulation modulates the inflammation after corneal epithelial abrasion Pubmed:35421396
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