ELISA Kit for Beta-Crosslaps (bCTx)

b-CTx; bCTXI; bCTX-I; B-Cr; BCL; Type I Collagen C-Telopeptide-Related Fraction


This assay has high sensitivity and excellent specificity for detection of Beta-Crosslaps (bCTx).
No significant cross-reactivity or interference between Beta-Crosslaps (bCTx) and analogues was observed.


Matrices listed below were spiked with certain level of recombinant Beta-Crosslaps (bCTx) and the recovery rates were calculated by comparing the measured value to the expected amount of Beta-Crosslaps (bCTx) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 92-99 95
EDTA plasma(n=5) 89-103 99
heparin plasma(n=5) 78-104 83


Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Beta-Crosslaps (bCTx) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Beta-Crosslaps (bCTx) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%


The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Beta-Crosslaps (bCTx) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 98-105% 80-92% 89-104% 89-97%
EDTA plasma(n=5) 97-105% 82-103% 78-97% 97-105%
heparin plasma(n=5) 99-105% 84-91% 95-103% 96-104%


The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1 Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
Reagent Diluent 1×300µL Stop Solution 1×6mL
TMB Substrate 1×9mL Instruction manual 1
Wash Buffer (30 × concentrate) 1×20mL

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.



Magazine Citations
Bosnian Journal of Basic Medical Sciences Effects of carbamazepine on serum parathormone, 25-hydroxyvitamin D, bone specific alkaline phosphatase, C-telopeptide, and osteocalcin levels in healthy rats PubMed: 23198939
Clin Cancer Res. Cabozantinib inhibits prostate cancer growth and prevents tumor-induced bone lesions Pubmed:Pmc3946460
Bone Elevated serum soluble CD200 and CD200R as surrogate markers of bone loss under bed rest conditions Pubmed:24333170
J Bone Miner Metab. Effect of Urocortin on strength and microarchitecture of osteopenic rat femur Pubmed:24633537
Journal of Nutrition Cholecalciferol Supplementation Promotes Bone Turnover in Chinese Adults with Vitamin D Deficiency Pubmed:29897564
Journal of Functional Foods Effect of long-term administration of mangiferin from on bone metabolism in ovariectomized rats 10.1016:j.jff.2018.04.048
European Journal of Medical Research Biomechanical and histological analyses of the fracture healing process after direct or prolonged reduction Pubmed: 30180907
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
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