ELISA Kit for Adrenocorticotropic Hormone (ACTH)

Corticotropin; Adrenocorticotropin


This assay has high sensitivity and excellent specificity for detection of Adrenocorticotropic Hormone (ACTH).
No significant cross-reactivity or interference between Adrenocorticotropic Hormone (ACTH) and analogues was observed.


Matrices listed below were spiked with certain level of recombinant Adrenocorticotropic Hormone (ACTH) and the recovery rates were calculated by comparing the measured value to the expected amount of Adrenocorticotropic Hormone (ACTH) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 78-105 91
EDTA plasma(n=5) 88-97 93
heparin plasma(n=5) 97-105 101


Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Adrenocorticotropic Hormone (ACTH) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Adrenocorticotropic Hormone (ACTH) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%


The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Adrenocorticotropic Hormone (ACTH) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 87-101% 81-103% 79-89% 78-97%
EDTA plasma(n=5) 80-96% 90-99% 80-101% 90-103%
heparin plasma(n=5) 78-97% 86-98% 78-103% 80-91%


The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.



Magazine Citations
Respir Res. Intracerebroventricular injection of leukotriene B4 attenuates antigen-induced asthmatic response via BLT1 receptor stimulating HPA-axis in sensitized rats. PubMed: 20403205
Toxicology Letters Regulation of corticosterone secretion is modified by PFOS exposure at different levels of the hypothalamic–pituitary–adrenal axis in adult male rats Pubmed:24440345
Mol Med Rep. Ursolic acid suppresses growth and adrenocorticotrophic hormone secretion in AtT20 cells as a potential agent targeting adrenocorticotrophic hormone-producing pituitary adenoma. Pubmed:24682498
Peptides Differential stress response in rats subjected to chronic mild stress is accompanied by changes in CRH-family gene expression at the pituitary level Pubmed:25236411
Journal of Animal and Feed Sciences Interdependence of the peripheral metabolism of glucocorticoids and thyroid hormones under calorie deficit in rats at different ages Researchgate:Source
International Journal of Immunopathology & Pharmacology Sea buckthorn (Hippophae rhamnoides L.) oil protects against chronic stress-induced inhibitory function of natural killer cells in rats PubMed: 26684638
Cell Tissue Res CXCL10/CXCR3 signaling mediates inhibitory action by interferon-gamma on CRF-stimulated adrenocorticotropic hormone (ACTH) release PubMed: 26572542
Neuroscience Letters Electroacupuncture reCavia (Guinea pig )late hypothalamic–pituitary–adrenal axis and enhance hippocampal serotonin system in a rat model of depression Pubmed:26773866
Neural Regeneration Research Moxibustion upreCavia (Guinea pig )lates hippocampal progranulin expression. Pubmed:27212922
Environmental Science and Pollution Research Lycopene ameliorates atrazine-induced oxidative damage in adrenal cortex of male rats by activation of the Nrf2/HO-1 pathway Pubmed:27102619
Sci Rep. Hedgehog signaling activation induces stem cell proliferation and hormone release in the adult pituitary gland Pubmed:27109116
Sci Rep. High activity of the stress promoter contributes to susceptibility to stress in the tree shrew Pubmed:27125313
brain research bulletin Activation of ATP-sensitive potassium channel by iptakalim normalizes stress-induced HPA axis disorder and depressive behaviour by alleviating inflammation and oxidative stress in mouse hypothalamus. pubmed:28161195
中国临床药理学与治疗学 肉蔻五味丸抗抑郁作用及机制研究 9787
Neuroscience Heat stress-induced neuroinflammation and aberration in monoamine levels in hypothalamus are associated with temperature dysregulation pubmed:28663093
Toxicology Mixture effects of azole fungicides on the adrenal gland in a broad dose range pubmed:28454766
Biomedicine & Pharmacotherapy Triptolide suppresses growth and hormone secretion in murine pituitary corticotroph tumor cells via NF-kappaB signaling pathway pubmed:28892788
Hormones and Behavior Acth-induced model of depression resistant to tricyclic antidepressants: Neuroendocrine and behavioral changes and influence of long-term magnesium … Pubmed:30025718
Frontiers in Pharmacology Vaccinium bracteatum Leaf Extract Reverses Chronic Restraint Stress-Induced Depression-Like Behavior in Mice: Regulation of Hypothalamic-Pituitary … Pubmed:30038568
Molecular and Cellular Endocrinology Metformin suppresses growth and adrenocorticotrophic hormone secretion in mouse pituitary corticotroph tumor AtT20 cells 10.1016:j.mce.2018.07.007
Frontiers in Behavioral Neuroscience Chronic hippocampal abnormalities and blunted HPA axis in an animal model of repeated unpredictable stress Pubmed:30079015
Placenta Human placentophagy: Effects of dehydration and steaming on hormones, metals and bacteria in placental tissue Pubmed:29941176
BMC Veterinary Research Hypothalamus-pituitary-adrenal axis involves in anti-viral ability through regulation of immune response in piglets infected by highly pathogenic porcine … Pubmed:29540178
European Journal of Applied Physiology Comparison between men and women of volume regulating hormones and aquaporin-2 excretion following graded central hypovolemia Pubmed: 30564880
Serbian Journal of Experimental and Clinical Research Assessment of the TDCS Influence on Stress-Induced Disorders in Rats with Low Stress Sustainability and Endurance
Scientific Reports Mice lacking Casp1, Ifngr and Nos2 genes exhibit altered depressive-and anxiety-like behaviour, and gut microbiome composition Pubmed: 31015500
alcoholism-clinical and experimental research Persistent changes in stress‐regulatory genes in pregnant woman or a child with prenatal alcohol exposure Pubmed: 31329297
Hormones and Behavior Hypothalamus-pituitary-adrenal axis imbalance and inflammation contribute to sex differences in separation-and restraint-induced depression Pubmed: 32165183
Prog Neuropsychopharmacol Biol Psychiatry Progress in Neuropsychopharmacology & Biological Psychiatry Pubmed: 17134810
Evid Based Complement Alternat Med So-Ochim-Tang-Gamibang, a Traditional Herbal Formula, Ameliorates Depression by Regulating Hyperactive Glucocorticoid Signaling In Vitro and In Vivo Pubmed: 33224257
PROGRESS IN NEURO-PSYCHOPHARMACOLOGY & BIOLOGICAL PSYCHIATRY Repeated unpredictable stress and social isolation induce chronic HPA axis dysfunction and persistent abnormal fear memory Pubmed: 32682873
Aging Cell Altered glucocorticoid metabolism represents a feature of macroph‐aging Pubmed: 32463582
neurochemical research Anxiolytic and Anti‑depressive Like Effects of Translocator Protein (18 kDa) Ligand YL‑IPA08 in a Rat Model of Postpartum Depression Pubmed: 32383026
Behavioral Ecology and Sociobiology  Exposure to eagle owl feces induces anti-predator behavior, physiology, and hypothalamic gene responses in a subterranean rodent, the plateau zokor (Eospalax …
EUROPEAN CHILD & ADOLESCENT PSYCHIATRY Increased serum brain-derived neurotrophic factor and adrenocorticotropic hormone levels are associated with obsessive compulsive disorder in medication?free?¡­ 33389158
International Journal of?Nanomedicine Water-Soluble Carbon Dots in Cigarette Mainstream Smoke: Their Properties and the Behavioural, Neuroendocrinological, and Neurotransmitter Changes They ¡­ 33762821
J Am Heart Assoc Post©\Cardiac Arrest Hydrocortisone Use Ameliorates Cardiac Mitochondrial Injury in a Male Rat Model of Ventricular Fibrillation Cardiac Arrest 33960200
Effects of Propofol versus Sevoflurane on Postoperative Pain and Neuroendocrine Stress Response in Oocyte Pickup Patients 33936350
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
CPA836Hu11 BSA Conjugated Adrenocorticotropic Hormone (ACTH) Immunogen; SDS-PAGE; WB.
CPA836Hu21 OVA Conjugated Adrenocorticotropic Hormone (ACTH) Immunogen; SDS-PAGE; WB.
PAA836Hu08 Polyclonal Antibody to Adrenocorticotropic Hormone (ACTH) WB; IHC; ICC; IP.
LAA836Hu81 FITC-Linked Polyclonal Antibody to Adrenocorticotropic Hormone (ACTH) WB; IHC; ICC; IF.
MAA836Hu21 Monoclonal Antibody to Adrenocorticotropic Hormone (ACTH) WB; IHC; ICC; IP.
MAA836Hu28 Monoclonal Antibody to Adrenocorticotropic Hormone (ACTH) WB; IHC; ICC; IP.
CEA836Hu ELISA Kit for Adrenocorticotropic Hormone (ACTH) Enzyme-linked immunosorbent assay for Antigen Detection.
CCA836Hu CLIA Kit for Adrenocorticotropic Hormone (ACTH) Chemiluminescent immunoassay for Antigen Detection.
LMA836Hu Magnetic Luminex Assay Kit for Adrenocorticotropic Hormone (ACTH) ,etc. Magnetic Luminex Assay for Antigen Detection.
KSA836Hu11 ELISA Kit DIY Materials for Adrenocorticotropic Hormone (ACTH) Main materials for "Do It (ELISA Kit) Yourself".