ELISA Kit for Adrenocorticotropic Hormone (ACTH)
- Product No.CEA836Cp
- Organism SpeciesCapra hircus; Caprine (Goat) Same name, Different species.
- Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodCompetitive Inhibition
- Assay Length2h
- Detection Range12.35-1000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 4.92pg/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
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This assay has high sensitivity and excellent specificity for detection of Adrenocorticotropic Hormone (ACTH).
No significant cross-reactivity or interference between Adrenocorticotropic Hormone (ACTH) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Adrenocorticotropic Hormone (ACTH) and the recovery rates were calculated by comparing the measured value to the expected amount of Adrenocorticotropic Hormone (ACTH) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Adrenocorticotropic Hormone (ACTH) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Adrenocorticotropic Hormone (ACTH) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Adrenocorticotropic Hormone (ACTH) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.
|Respir Res.||Intracerebroventricular injection of leukotriene B4 attenuates antigen-induced asthmatic response via BLT1 receptor stimulating HPA-axis in sensitized rats. PubMed: 20403205|
|Toxicology Letters||Regulation of corticosterone secretion is modified by PFOS exposure at different levels of the hypothalamic–pituitary–adrenal axis in adult male rats Pubmed:24440345|
|Mol Med Rep.||Ursolic acid suppresses growth and adrenocorticotrophic hormone secretion in AtT20 cells as a potential agent targeting adrenocorticotrophic hormone-producing pituitary adenoma. Pubmed:24682498|
|Peptides||Differential stress response in rats subjected to chronic mild stress is accompanied by changes in CRH-family gene expression at the pituitary level Pubmed:25236411|
|Journal of Animal and Feed Sciences||Interdependence of the peripheral metabolism of glucocorticoids and thyroid hormones under calorie deficit in rats at different ages Researchgate:Source|
|International Journal of Immunopathology & Pharmacology||Sea buckthorn (Hippophae rhamnoides L.) oil protects against chronic stress-induced inhibitory function of natural killer cells in rats PubMed: 26684638|
|Cell Tissue Res||CXCL10/CXCR3 signaling mediates inhibitory action by interferon-gamma on CRF-stimulated adrenocorticotropic hormone (ACTH) release PubMed: 26572542|
|Neuroscience Letters||Electroacupuncture reCavia (Guinea pig )late hypothalamic–pituitary–adrenal axis and enhance hippocampal serotonin system in a rat model of depression Pubmed:26773866|
|Neural Regeneration Research||Moxibustion upreCavia (Guinea pig )lates hippocampal progranulin expression. Pubmed:27212922|
|Environmental Science and Pollution Research||Lycopene ameliorates atrazine-induced oxidative damage in adrenal cortex of male rats by activation of the Nrf2/HO-1 pathway Pubmed:27102619|
|Sci Rep.||Hedgehog signaling activation induces stem cell proliferation and hormone release in the adult pituitary gland Pubmed:27109116|
|Sci Rep.||High activity of the stress promoter contributes to susceptibility to stress in the tree shrew Pubmed:27125313|
|brain research bulletin||Activation of ATP-sensitive potassium channel by iptakalim normalizes stress-induced HPA axis disorder and depressive behaviour by alleviating inflammation and oxidative stress in mouse hypothalamus. pubmed:28161195|
|Neuroscience||Heat stress-induced neuroinflammation and aberration in monoamine levels in hypothalamus are associated with temperature dysregulation pubmed:28663093|
|Toxicology||Mixture effects of azole fungicides on the adrenal gland in a broad dose range pubmed:28454766|
|Biomedicine & Pharmacotherapy||Triptolide suppresses growth and hormone secretion in murine pituitary corticotroph tumor cells via NF-kappaB signaling pathway pubmed:28892788|
|Hormones and Behavior||Acth-induced model of depression resistant to tricyclic antidepressants: Neuroendocrine and behavioral changes and influence of long-term magnesium … Pubmed:30025718|
|Frontiers in Pharmacology||Vaccinium bracteatum Leaf Extract Reverses Chronic Restraint Stress-Induced Depression-Like Behavior in Mice: Regulation of Hypothalamic-Pituitary … Pubmed:30038568|
|Molecular and Cellular Endocrinology||Metformin suppresses growth and adrenocorticotrophic hormone secretion in mouse pituitary corticotroph tumor AtT20 cells 10.1016:j.mce.2018.07.007|
|Frontiers in Behavioral Neuroscience||Chronic hippocampal abnormalities and blunted HPA axis in an animal model of repeated unpredictable stress Pubmed:30079015|
|Placenta||Human placentophagy: Effects of dehydration and steaming on hormones, metals and bacteria in placental tissue Pubmed:29941176|
|BMC Veterinary Research||Hypothalamus-pituitary-adrenal axis involves in anti-viral ability through regulation of immune response in piglets infected by highly pathogenic porcine … Pubmed:29540178|
|European Journal of Applied Physiology||Comparison between men and women of volume regulating hormones and aquaporin-2 excretion following graded central hypovolemia Pubmed: 30564880|
|Cyber Leninka||ТЭС-ТЕРАПИЯ КАК МЕТОД ПРЕДУПРЕЖДЕНИЯ ДЕЗАДАПТАЦИИ У САМЦОВ КРЫС С ВЫСОКОЙ СТРЕССОУСТОЙЧИВОСТЬЮ|
|Serbian Journal of Experimental and Clinical Research||Assessment of the TDCS Influence on Stress-Induced Disorders in Rats with Low Stress Sustainability and Endurance|
|Scientific Reports||Mice lacking Casp1, Ifngr and Nos2 genes exhibit altered depressive-and anxiety-like behaviour, and gut microbiome composition Pubmed: 31015500|
|alcoholism-clinical and experimental research||Persistent changes in stress‐regulatory genes in pregnant woman or a child with prenatal alcohol exposure Pubmed: 31329297|
|Catalog No.||Related products for research use of Capra hircus; Caprine (Goat) Organism species||Applications (RESEARCH USE ONLY!)|
|CEA836Cp||ELISA Kit for Adrenocorticotropic Hormone (ACTH)||Enzyme-linked immunosorbent assay for Antigen Detection.|