ELISA Kit for Beta-Endorphin (bEP)



This assay has high sensitivity and excellent specificity for detection of Beta-Endorphin (bEP).
No significant cross-reactivity or interference between Beta-Endorphin (bEP) and analogues was observed.


Matrices listed below were spiked with certain level of recombinant Beta-Endorphin (bEP) and the recovery rates were calculated by comparing the measured value to the expected amount of Beta-Endorphin (bEP) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 84-101 87
EDTA plasma(n=5) 82-96 90
heparin plasma(n=5) 98-105 102


Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Beta-Endorphin (bEP) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Beta-Endorphin (bEP) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%


The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Beta-Endorphin (bEP) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 82-101% 94-104% 81-89% 91-105%
EDTA plasma(n=5) 78-91% 80-95% 80-102% 95-104%
heparin plasma(n=5) 85-94% 85-103% 96-103% 82-89%


The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1 Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
Reagent Diluent 1×300µL Stop Solution 1×6mL
TMB Substrate 1×9mL Instruction manual 1
Wash Buffer (30 × concentrate) 1×20mL

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.



Magazine Citations
Experimental Dermatology Endogenous μ-opioid peptides modulate immune response towards malignant melanoma Publish: RD10279
European Journal of Physiology Physiology and cell biology of acupuncture observed in calcium signaling activated by acoustic shear wave SpringerLink: tv015l3126833315
International Journal of Pharmaceutical Science Invention ISSN(Online) Effect of Ramadan fasting on endorphin and endocannabinoid level in serum, PBMC and macrophage Ijpsi: Source
Neurological Sciences Evaluation of immune parameters in chronic migraine with medication overuse Pubmed:24867859
Brain Behav. Cerebrospinal fluid and plasma b-endorphin levels in children with cerebral malaria. pubmed:28413714
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Journal of Pediatric Neurosciences Plasma and cerebrospinal fluid beta-endorphin levels show a strong association in children with cerebral malaria
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JOURNAL OF AFFECTIVE DISORDERS Pain Sensitivity and Plasma Beta-Endorphin in Adolescent Non-Suicidal Self-Injury Pubmed: 32961416
EUROPEAN NEUROPSYCHOPHARMACOLOGY Serum β-endorphin levels are associated with addiction to suicidal behavior: a pilot study Pubmed: 32855024
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