ELISA Kit for Inhibin B (INHB)



This assay has high sensitivity and excellent specificity for detection of Inhibin B (INHB).
No significant cross-reactivity or interference between Inhibin B (INHB) and analogues was observed.


Matrices listed below were spiked with certain level of recombinant Inhibin B (INHB) and the recovery rates were calculated by comparing the measured value to the expected amount of Inhibin B (INHB) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 95-103 98
EDTA plasma(n=5) 78-96 89
heparin plasma(n=5) 92-101 96


Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Inhibin B (INHB) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Inhibin B (INHB) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%


The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Inhibin B (INHB) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 78-97% 84-104% 96-103% 78-102%
EDTA plasma(n=5) 78-93% 86-102% 86-101% 91-98%
heparin plasma(n=5) 85-104% 89-97% 96-103% 83-101%


The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1 Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
Reagent Diluent 1×300µL Stop Solution 1×6mL
TMB Substrate 1×9mL Instruction manual 1
Wash Buffer (30 × concentrate) 1×20mL

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.



Magazine Citations
Adv Med Sci. Are anti-MĂźllerian hormone and its receptor polymorphism associated with the hormonal condition of undescended testes? Pubmed:27162065
Chemosphere. Gestational and lactational exposure to bisphenol AF in maternal rats increases testosterone levels in 23-day-old male offspring. pubmed:27567155
PLoS One Overexpression of ubiquitin carboxyl-terminal hydrolase 1 (UCHL1) in boys with cryptorchidism Pubmed:29401475
Journal of Ovarian Research Comparison of the influence of three fibroid treatment options: supracervical hysterectomy, ulipristal acetate and uterine artery embolization on ovarian … Pubmed:29859107
Stem Cell Research & Therapy Endometrial mesenchymal stem cells isolated from menstrual blood repaired epirubicin-induced damage to human ovarian granulosa cells by inhibiting the … Pubmed: 30606243
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