ELISA Kit for Brain Natriuretic Peptide (BNP)

GC-B; B-Type Natriuretic Peptide; Ventricular Natriuretic Peptide; Gamma-brain natriuretic peptide; Brain natriuretic peptide 32


This assay has high sensitivity and excellent specificity for detection of Brain Natriuretic Peptide (BNP).
No significant cross-reactivity or interference between Brain Natriuretic Peptide (BNP) and analogues was observed.


Matrices listed below were spiked with certain level of recombinant Brain Natriuretic Peptide (BNP) and the recovery rates were calculated by comparing the measured value to the expected amount of Brain Natriuretic Peptide (BNP) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 90-98 94
EDTA plasma(n=5) 78-92 80
heparin plasma(n=5) 82-95 88


Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Brain Natriuretic Peptide (BNP) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Brain Natriuretic Peptide (BNP) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%


The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Brain Natriuretic Peptide (BNP) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 86-97% 81-91% 85-101% 98-105%
EDTA plasma(n=5) 81-92% 87-94% 92-101% 92-99%
heparin plasma(n=5) 94-103% 98-105% 79-96% 78-98%


The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.



Magazine Citations
Heart and Circulatory Physiology B-type natriuretic peptide, vascular endothelial growth factor, endothelin-1, and nitric oxide synthase in chronic mountain sickness APS: source
PLoS One Renalase deficiency in heart failure model of rats_a potential mechanism underlying circulating norepinephrine accumulation. PubMed: 21297953
International Journal of Toxicology Cardiac Troponin T and NT-proBNP as Biomarkers of Early Myocardial Damage in Amitriptyline-Induced Cardiovascular Toxicity in Rats Pubmed: 24055824
J Urol.? Why does Adenotonsillectomy Not Correct Enuresis in All Children with Sleep Disordered Breathing? Pubmed:24679871
Life Sci Cardioprotective effects of baicalein on heart failure via modulation of Ca2 PubMed: 26706290
Vlaams Diergeneeskundig Tijdschrift Equine BNP measurement using a porcine BNP enzyme-linked immunoassay: a pilot study Publication: 5950761
Physiological Research Renal Sympathetic Denervation Improves Cardiac Dysfunction PubMed: 25804096
Life Sci Cardioprotective effects of baicalein on heart failure via modulation of Ca(2+) handling proteins in vivo and in vitro Pubmed:26706290
J Ethnopharmacol Danhong injection attenuates isoproterenol-induced cardiac hypertrophy by reCavia (Guinea pig )lating p38 and NF-κb pathway Pubmed:26970569
Echocardiography. Improvement in the prediction of exercise-induced elevation of left ventricular filling pressure in patients with normal left ventricular ejection fraction. pubmed:27779336
SpringerPlus The effect of long-term amiodarone administration on myocardial fibrosis and evolution of left ventricular remodeling in a porcine model of ischemic … pubmed:27652141
Heart Lung Circ. Efficacy and Safety of Renal Sympathetic Denervation on Dogs with Pressure Overload-Induced Heart Failure. pubmed:27555054
Clinica Chimica Acta  Plasma concentration of fibronectin is decreased in patients with hypertrophic cardiomyopathy pubmed:27693530
Scientific Reports Paracrine cross-talk between skeletal muscle and macrophages in exercise by PGC-1α-controlled BNP. pubmed:28091624
Molecular Medicine Report Intermedin improves cardiac function and sympathetic neural remodeling in a rat model of post myocardial infarction heart failure pubmed:28627670
PLoS One Enhanced heart failure, mortality and renin activation in female mice with experimental dilated cardiomyopathy pubmed:29240788
Acta Physiologica Beneficial Effects of Renal Denervation on Cardiac Angiogenesis in Rats with Prolonged Pressure Overload pubmed:27575955
International Immunopharmacology Metformin attenuated endotoxin-induced acute myocarditis via activating AMPK pubmed:28410530
Drug Delivery Ginsenoside Rg3 micelles mitigate doxorubicin-induced cardiotoxicity and enhance its anticancer efficacy. pubmed:29063791
Science of the Total Environment Co-exposure of silica nanoparticles and methylmercury induced cardiac toxicity and Pubmed:29727991
Scientific Reports An essential role for Wnt/β-catenin signaling in mediating hypertensive heart disease Pubmed:29895976
International Journal of Cardiology Cardioprotective effect of substance P in a porcine model of acute myocardial infarction Pubmed:29884290
International Journal of Cardiology High-intensity interval training enhances mitochondrial bioenergetics of platelets in patients with heart failure Pubmed:30072155
Evidence-based Complementary and Alternative Medicine  Acute Biochemical, Cardiovascular, and Autonomic Response to Hyperbaric (4 atm) Exposure in Healthy Subjects Pubmed:29977313
Molecular Medicine Reports  Hydrogen‑rich solution against myocardial injury and aquaporin expression via the PI3K/Akt signaling pathway during cardiopulmonary bypass in rats Pubmed:29956781
Pediatric Cardiology Determinants of Increased Aortic Diameters in Young Normotensive Patients With Turner Syndrome Without Structural Heart Disease Pubmed:29392348
BMC Pharmacology and Toxicology Enoxaparin attenuates doxorubicin induced cardiotoxicity in rats via interfering with oxidative stress, inflammation and apoptosis Pubmed:29321061
中国病理生理杂志 和肽素水平对大鼠心肾综合征的早期预测价值 filename:ZBLS201802013
FASEB Journal Signature-oriented investigation of the efficacy of multicomponent drugs against heart failure Pubmed: 30230922
Toxicology Mechanisms and Methods Long-term exposure to crotonaldehyde causes heart and kidney dysfunction through induction of inflammatory and oxidative damage in male Wistar rats Pubmed: 30426860
Van Veterinary Journal Van Veterinary Journal
Kidney International Wnt/β-catenin signaling mediates both heart and kidney injury in type 2 cardiorenal syndrome Pubmed: 30770217
Journal of Cellular Physiology Inflammatory cytokines associated with cancer growth induce mitochondria and cytoskeleton alterations in cardiomyocytes Pubmed: 30982981
Catalog No. Related products for research use of Chicken (Gallus) Organism species Applications (RESEARCH USE ONLY!)
CEA541Ga ELISA Kit for Brain Natriuretic Peptide (BNP) Enzyme-linked immunosorbent assay for Antigen Detection.