ELISA Kit for Prostaglandin E2 (PGE2)
- Product No.CEA538Ge
- Organism SpeciesPan-species (General) Same name, Different species.
- Sample Typeserum, plasma and other biological fluids
- Test MethodCompetitive Inhibition
- Assay Length2h
- Detection Range2.47-200pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 1.13pg/mL.
- DownloadInstruction Manual
96T*5 96T*10 96T*100
For more details, please contact local distributors! US$ 796
For more details, please contact local distributors! US$ 3580
For more details, please contact local distributors! US$ 6763
For more details, please contact local distributors! US$ 55692
For more details, please contact local distributors!
This assay has high sensitivity and excellent specificity for detection of Prostaglandin E2 (PGE2).
No significant cross-reactivity or interference between Prostaglandin E2 (PGE2) and analogues was observed.
Matrices listed below were spiked with certain level of Prostaglandin E2 (PGE2) and the recovery rates were calculated by comparing the measured value to the expected amount of Prostaglandin E2 (PGE2) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Prostaglandin E2 (PGE2) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Prostaglandin E2 (PGE2) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Prostaglandin E2 (PGE2) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|Reagent Diluent||1×300µL||Stop Solution||1×6mL|
|TMB Substrate||1×9mL||Instruction manual||1|
|Wash Buffer (30 × concentrate)||1×20mL|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.
|Immunopharmacology and Inflammation||Evidence for the complementary and synergistic effects of the three-alkaloid combination regimen containing berberine, hypaconitine and skimmianine on the ulcerative colitis rats induced by trinitrobenzene-sulfonic acid ScienceDirect: S0188440910001049|
|PLoS ONE||Celecoxib Ameliorates Portal Hypertension of the Cirrhotic Rats through the Dual Inhibitory Effects on the Intrahepatic Fibrosis and Angiogenesis PubMed: PMC3724827|
|Lipids in Health and Disease||Lipid mediators in plasma of autism spectrum disorders PubMed: PMC3557222|
|Journal of Thoracic Oncology||Interleulin-17 and Prostaglandin E2 Are Involved in Formation of an M2 Macrophage-dominant Microenvironment in Lung Cancer PubMed: PMC3378786|
|Cell biology international,||Artesunate exerts an anti-immunosuppressive effect on cervical cancer by inhibiting PGE2 production and Foxp3 expression. Pubmed: 24446394|
|Journal of ethnopharmacology||Multitargeted protective effect of Abacopteris penangiana against carrageenan-induced chronic prostatitis in rats. Pubmed: 24211397|
|Journal of Gastroenterology and Hepatology||Celecoxib attenuates hepatic cirrhosis through inhibition of epithelial-to-mesenchymal transition of hepatocytes Pubmed:24909904|
|J Surg Res.||Valproic acid protects septic mice from renal injury by reducing the inflammatory response Pubmed:24948542|
|Inflammation.?||Alpha-lipoic acid exerts anti-inflammatory effects on lipopolysaccharide-stimulated rat mesangial cells via inhibition of nuclear factor kappa B (NF-κB) signaling pathway. Pubmed:24962643|
|Mol Med Rep||Somatostatin prevents lipopolysaccharide PubMed: 25777539|
|Eur J Pharmacol||Celecoxib enhanced the cytotoxic effect of cisplatin in drug-resistant human gastric cancer cells by inhibition of cyclooxygenase-2 PubMed: 26407653|
|PLOS ONE||Penehyclidine Hydrochloride Preconditioning Provides Cardioprotection in a Rat Model of Myocardial Ischemia/Reperfusion Injury PubMed: 26632817|
|Immunologic Research||Mesenchymal stromal cell-dependent reprogramming of Kupffer cells is mediated by TNF-α and PGE9 and is crucial for liver transplant tolerance PubMed: 25982596|
|Arquivos Brasileiros de Oftalmologia||Effects of morphine on the expression of cytokines and inflammatory mediators in a rabbit model of endotoxin-induced experimental uveitis PubMed: 26677041|
|J Breath Res||Effects of different ventilation strategies on exhaled nitric oxide in geriatric abdominal surgery PubMed: 25719610|
|J Surg Res||Artemisinin inhibits the proliferation, migration, and inflammatory reaction induced by tumor necrosis factor-伪 in vascular smooth muscle cells through nuclear factor kappa B pathway PubMed: 25575733|
|Tumor Biology||Prostaglandin E2 (PGE2) promotes proliferation and invasion by enhancing SUMO-1 activity via EP4 receptor in endometrial cancer Pubmed:27230680|
|Arthritis Research & Therapy||IL-23/Th17 axis is not influenced by TNF-blocking agents in ankylosing spondylitis patients Pubmed:26912133|
|Oncotarget||Metformin represses bladder cancer progression by inhibiting stem cell repopulation via COX2/PGE2/STAT3 axis Pubmed:27058422|
|Am J Physiol Gastrointest Liver Physiol||Inhibition of cyclooxygenase-2 alleviates liver cirrhosis via improvement of the dysfunctional Cavia (Guinea pig )t-liver axis in rats Pubmed:27056726|
|Oncotarget||Metformin represses bladder cancer progression by inhibiting stem cell repopulation via COX2/PGE2/STAT3 axis. pubmed:27058422|
|Lipids.||Dietary Arachidonic Acid Has a Time-Dependent Differential Impact on Adipogenesis Modulated via COX and LOX Pathways in Grass Carp Ctenopharyngodon idellus. pubmed:27747453|
|Fish Physiology and Biochemistry||Role of cyclooxygenase-mediated metabolites in lipid metabolism and expression of some immune-related genes in juvenile grass carp (Ctenopharyngodon idellus) fed arachidonic acid. pubmed:28012026|
|Cancer Letters||Metformin inhibits castration-induced EMT in prostate cancer by repressing COX2/PGE2/STAT3 axis. pubmed:28043910|
|PLOS ONE||Identification of Biomarkers of Impaired Sensory Profiles among Autistic Patients. pubmed:27824861|
|Scientific Reports||A Zinc Morpholine Complex Prevents HCl/Ethanol-Induced Gastric Ulcers in a Rat Model. pubmed:27460157|
|PLoS One.||Downregulation of HOTAIR Expression Mediated Anti-Metastatic Effect of Artesunate on CervicalCancer by Inhibiting COX-2 Expression. pubmed:27736969|
|Food Funct.||Flavonoids from sea buckthorn inhibit the lipopolysaccharide-induced inflammatory response in RAW264.7 macrophages through the MAPK and NF-κB pathways. pubmed:28256654|
|Catalog No.||Related products for research use of Pan-species (General) Organism species||Applications (RESEARCH USE ONLY!)|
|CEA538Ge||ELISA Kit for Prostaglandin E2 (PGE2)||Enzyme-linked immunosorbent assay for Antigen Detection.|