ELISA Kit for Glutathione (GSH)
- Product No.CEA294Ge
- Organism SpeciesPan-species (General) Same name, Different species.
- Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodCompetitive Inhibition
- Assay Length2h
- Detection Range1.23-100ug/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.45ug/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
For more details, please contact local distributors! US$ 796
For more details, please contact local distributors! US$ 3580
For more details, please contact local distributors! US$ 6763
For more details, please contact local distributors! US$ 55692
For more details, please contact local distributors!
This assay has high sensitivity and excellent specificity for detection of Glutathione (GSH).
No significant cross-reactivity or interference between Glutathione (GSH) and analogues was observed.
Matrices listed below were spiked with certain level of Glutathione (GSH) and the recovery rates were calculated by comparing the measured value to the expected amount of Glutathione (GSH) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glutathione (GSH) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glutathione (GSH) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Glutathione (GSH) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|Reagent Diluent||1×300µL||Stop Solution||1×6mL|
|TMB Substrate||1×9mL||Instruction manual||1|
|Wash Buffer (30 × concentrate)||1×20mL|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.
|European Journal of Clinical Nutrition (2012) 66||Evidence for augmented oxidative stress in the subjects with type 1 diabetes and their siblings: a possible preventive role for antioxidants PubMed: 22781023|
|International Journal of Medical Sciences||Glutathione S-Transferase P1 Correlated with Oxidative Stress in Hepatocellular Carcinoma PubMed: PMC3619117|
|Reproduction||Alteration in the intrafollicular thiol–redox system in infertile women with endometriosis Pubmed:25376627|
|Iran J Basic Med Sci||Effects of mild hypothermia therapy on the levels of glutathione in rabbit blood and cerebrospinal fluid after cardiopulmonary resuscitation PubMed: 25810895|
|Bulletin of the Veterinary Institute in Pulawy||Oxidative Stability of Ostrich Meat Related to Duration of Linseed and Lucerne Supplementation to the Bird's Diet View: J|
|Journal of Allergy and Clinical Immunology||Prenatal maternal distress affects atopic dermatitis in offspring mediated by oxidative stress Pubmed:27016803|
|Oxidative Medicine and Cellular Longevity||Hydrogen sulfide protects against chronic unpredictable mild stress-induced oxidative stress in hippocampus by upreCavia (Guinea pig )lation of BDNF-TrkB pathway journals:2153745.pdf|
|Institutional repository of Vilnius University||Rapid opioid detoxification with an implementation of the new method of gradually increasing dosage of naltrexone induction object:elaba:15733325|
|Molecular Medicine Reports||Toxicity study of oxalicumone A, derived from a marine-derived fungus Penicillium oxalicum, in cultured renal epithelial cells pubmed:28260084|
|Molecular Medicine Reports||Hepatocellular toxicity of oxalicumone A via oxidative stress injury and mitochondrial dysfunction in healthy human liver cells pubmed:29115483|
|Cancer Medicine||Metabolomic and transcriptomic profiling of hepatocellular carcinomas in Hras12V transgenic mice pubmed:28941178|
|Free radical research||Circulating cell-free DNA of methylated insulin-like growth factor-binding protein 7 predicts a poor prognosis in hepatitis B virus-associated hepatocellular carcinoma … Pubmed:29463155|
|Biomed Research International||Epigallocatechin-3-Gallate Reduces Neuronal Apoptosis in Rats after Middle Cerebral Artery Occlusion Injury via PI3K/AKT/eNOS Signaling Pathway Pubmed:29770336|
|Molecular Medicine Reports||Epigallocatechingallate attenuates myocardial injury in a mouse model of heart failure through TGF‑β1/Smad3 signaling pathway Pubmed:29620209|
|Experimental and Therapeutic Medicine||Effects of ginsenoside Rb1 on oxidative stress injury in rat spinal cords by regulating the eNOS/Nrf2/HO‑1 signaling pathway Pubmed:30116359|
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