CLIA Kit for Inositol Triphosphate (IP3)

InsP3; Inositol 1,4,5-Trisphosphate; Triphosphoinositol


This assay has high sensitivity and excellent specificity for detection of Inositol Triphosphate (IP3).
No significant cross-reactivity or interference between Inositol Triphosphate (IP3) and analogues was observed.


Matrices listed below were spiked with certain level of Inositol Triphosphate (IP3) and the recovery rates were calculated by comparing the measured value to the expected amount of Inositol Triphosphate (IP3) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 97-104 101
EDTA plasma(n=5) 89-96 92
heparin plasma(n=5) 87-99 94


Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Inositol Triphosphate (IP3) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Inositol Triphosphate (IP3) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%


The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Inositol Triphosphate (IP3) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 79-95% 82-97% 94-101% 93-105%
EDTA plasma(n=5) 88-101% 84-104% 80-95% 85-96%
heparin plasma(n=5) 85-93% 92-101% 92-103% 95-104%


The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
Substrate A 1×10mL Substrate B 1×2mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
7. Read RLU value immediately.



Magazine Citations
Molecular Medicine Reports The association between the expression of PAR2 and TMEM16A and neuropathic pain Pubmed:29257338
Journal of Biological Chemistry OSBP-related protein 4L promotes phospholipase Cβ3 translocation from the nucleus to the plasma membrane in Jurkat T-cells Pubmed: 30237164
Catalog No. Related products for research use of Pan-species (General) Organism species Applications (RESEARCH USE ONLY!)
CEC037Ge ELISA Kit for Inositol Triphosphate (IP3) Enzyme-linked immunosorbent assay for Antigen Detection.
CCC037Ge CLIA Kit for Inositol Triphosphate (IP3) Chemiluminescent immunoassay for Antigen Detection.