Packages (Simulation)
Reagent Preparation
Image (I)
-
Results demonstration
Image (II)
-
Typical Standard Curve
Quality Guarantee
Instruction Manual
Certificate
CLIA Kit for Inhibin B (INHB)
INH-B
- Product No.CCA760Mu
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodCompetitive Inhibition
- Assay Length2h
- Detection Range3.9-1,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 1.4pg/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
- FOB
US$ 575
US$ 821
US$ 3695
US$ 6979
US$ 57470
For more details, please contact local distributors!
Specificity
This assay has high sensitivity and excellent specificity for detection of Inhibin B (INHB).
No significant cross-reactivity or interference between Inhibin B (INHB) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Inhibin B (INHB) and the recovery rates were calculated by comparing the measured value to the expected amount of Inhibin B (INHB) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 79-97 | 90 |
| EDTA plasma(n=5) | 96-104 | 101 |
| heparin plasma(n=5) | 93-104 | 99 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Inhibin B (INHB) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Inhibin B (INHB) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Inhibin B (INHB) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 78-102% | 78-91% | 98-105% | 97-104% |
| EDTA plasma(n=5) | 78-95% | 80-96% | 89-101% | 89-97% |
| heparin plasma(n=5) | 87-101% | 78-92% | 85-99% | 90-103% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1 | Assay Diluent A | 1×12mL |
| Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
| Reagent Diluent | 1×300µL | Stop Solution | 1×6mL |
| TMB Substrate | 1×9mL | Instruction manual | 1 |
| Wash Buffer (30 × concentrate) | 1×20mL |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
7. Read RLU value immediately.
GIVEAWAYS
INCREMENT SERVICES
| Magazine | Citations |
| Adv Med Sci. | Are anti-MĂźllerian hormone and its receptor polymorphism associated with the hormonal condition of undescended testes? Pubmed:27162065 |
| Chemosphere. | Gestational and lactational exposure to bisphenol AF in maternal rats increases testosterone levels in 23-day-old male offspring. pubmed:27567155 |
| PLoS One | Overexpression of ubiquitin carboxyl-terminal hydrolase 1 (UCHL1) in boys with cryptorchidism Pubmed:29401475 |
| Journal of Ovarian Research | Comparison of the influence of three fibroid treatment options: supracervical hysterectomy, ulipristal acetate and uterine artery embolization on ovarian … Pubmed:29859107 |
| Stem Cell Research & Therapy | Endometrial mesenchymal stem cells isolated from menstrual blood repaired epirubicin-induced damage to human ovarian granulosa cells by inhibiting the … Pubmed: 30606243 |
| Endocrinology | Diabetes Type 1 Negatively Influences Leydig Cell Function in Rats, Which is Partially Reversible By Insulin Treatment 33507237 |
| Catalog No. | Related products for research use of Mus musculus (Mouse) Organism species | Applications (RESEARCH USE ONLY!) |
| SEA760Mu | ELISA Kit for Inhibin B (INHB) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| CEA760Mu | ELISA Kit for Inhibin B (INHB) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| CCA760Mu | CLIA Kit for Inhibin B (INHB) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA760Mu | Multiplex Assay Kit for Inhibin B (INHB) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |