CLIA Kit for Luteinizing Hormone (LH)
ICSH; Lutropin; Interstitial Cell Stimulating Hormone
- Product No.CCA441Ra
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- Sample Typeserum, plasma and other biological fluids
- Test MethodCompetitive Inhibition
- Assay Length2h
- Detection Range195-50,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 70pg/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
For more details, please contact local distributors! US$ 760
For more details, please contact local distributors! US$ 3420
For more details, please contact local distributors! US$ 6460
For more details, please contact local distributors! US$ 53200
For more details, please contact local distributors!
This assay has high sensitivity and excellent specificity for detection of Luteinizing Hormone (LH).
No significant cross-reactivity or interference between Luteinizing Hormone (LH) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Luteinizing Hormone (LH) and the recovery rates were calculated by comparing the measured value to the expected amount of Luteinizing Hormone (LH) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Luteinizing Hormone (LH) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Luteinizing Hormone (LH) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Luteinizing Hormone (LH) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|Reagent Diluent||1×300µL||Substrate B||1×2mL|
|Substrate A||1×10mL||Instruction manual||1|
|Wash Buffer (30 × concentrate)||1×20mL|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
7. Read RLU value immediately.
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|Biochemical Journal||PPARγ co-activator-1α co-activates steroidogenic factor 1 to stimulate the synthesis of luteinizing hormone and aldosterone PubMed: 21108604|
|Molecular and Cellular Endocrinology||Effects of genistein and estrogen receptor subtype-specific agonists in ArKO mice following different administration routes ScienceDirect: S0303720709004043|
|Asian Journal of Andrology||Brief maternal exposure of rats to the xenobiotics dibutyl phthalate or diethylstilbestrol alters adult-type Leydig cell development in male offspring PubMed: 23314658|
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|Reproductive Toxicology||Inhibitory effects of mitotane on viability and secretory activity in mouse gonadotroph cell lines Pubmed: 24486453|
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|Journal of Materials Chemistry B||Nanocomposites of bio-based hyperbranched polyurethane/funtionalized MWCNT as non-immunogenic, osteoconductive, biodegradable and biocompatible scaffolds in bone tissue engineering Rsc: Source|
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|Academic Sciences||Piper longum hexane fraction induces infertility by modulation of inflammatory mediators and gonadotropin insufficiency in female rats Ijppsjournal: Source|
|Biochemical and Biophysical Research Communications||Critical function of RA-GEF-2/Rapgef6, a guanine nucleotide exchange factor for Rap1, in mouse spermatogenesis ScienceDirect: S0006291X14001843|
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|Andrologia||Protective effects of Eugenia jambolana extract versus N-acetyl cysteine against cisplatin-induced damage in rat testis Pubmed:24576220|
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|Genet Mol Biol.||Targeted disruption of the mouse testis-enriched gene Znf230 does not affect spermatogenesis or fertility Pubmed:25505846|
|Turkish Journal of Urology||Apoptosis in testicular tissue of rats after vasectomy: evaluation of eNOS, iNOS immunoreactivities and the effects of ozone therapy Turkishjournalofurology:Source|
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|European Journal of Pharmacology||The effect of R-(-)-deprenyl administration on reproductive parameters of rat males PubMed: 25725114|
|Andrologia||Dose‐and time‐dependent effects of Garcinia kola seed extract on sexual behaviour and reproductive parameters in male Wistar rats PubMed: 26123866|
|Reprod Toxicol||Simvastatin decreases steroid production in the H295R cell line and decreases steroids and FSH in female rats PubMed: 26476359|
|Biology of Reproduction||Maternal High-Fat Diet Induced Loss of Fetal Oocytes Is Associated with Compromised Follicle Growth in Adult Rat Offspring Pubmed:26962114|
|Andrologia||Dose- and time-dependent effects of Garcinia kola seed extract on sexual behaviour and reproductiveparameters in male Wistar rats. Pubmed:26123866|
|Int J Mol Sci.||Overexpression of PRL7D1 in Leydig Cells Causes Male Reproductive Dysfunction in Mice Pubmed:26771609|
|PLoS Genet.||A Jacob/Nsmf Gene Knockout Results in Hippocampal Dysplasia and Impaired BDNF Signaling in Dendritogenesis Pubmed:26977770|
|Scientific Reports||Molecular characterization of insulin resistance and glycolytic metabolism in the rat uterus pubmed:27461373|
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|Toxicological Sciences||Exposure of pregnant mice to perfluorobutanesulfonate causes hypothyroxinemia and developmental abnormalities in female offspring pubmed:27803384|
|Andrologia||Effects of treatment with Hypoxis hemerocallidea extract on sexual behaviour and reproductiveparameters in male rats. pubmed:28000943|
|Biology of Reproduction||Maternal High-Fat Diet-Induced Loss of Fetal Oocytes Is Associated with Compromised Follicle Growth in Adult Rat Offspring. pubmed:26962114|
|J Endocrinol.||Impact of uteroplacental insufficiency on postnatal rat male gonad. pubmed:27885054|
|Int J Pharm.||New gonadotropin-releasing hormone glycolipids with direct antiproliferative activity and gonadotropin-releasing potency. pubmed:28232269|
|Environmental Science & Technology||Pyrethroid Insecticide Cypermethrin Accelerates Pubertal Onset in Male Mice via Disrupting Hypothalamic-Pituitary-Gonadal Axis pubmed:28731686|
|Journal of Medicinal Chemistry||Development of New Gonadotropin-Releasing Hormone-Modified Dendrimer Platforms with Direct Antiproliferative and Gonadotropin Releasing Activity pubmed:28958147|
|Society of Toxicology||Pyrethroid Insecticide Cypermethrin Modulates Gonadotropin Synthesis via Calcium Homeostasis and ERK1/2 Signaling in LbT2 Mouse Pituitary Cells pubmed:29149324|
|Reproductive Toxicology||Vitamin D deficiency impairs testicular development and spermatogenesis in mice pubmed:28655646|
|The Journal of Biological Chemistry.||Lysophosphatidic acid acyltransferase 3 tunes the membrane status of germ cells by incorporating docosahexaenoic acid during spermatogenesis. pubmed:28578315|
|The Journal of Toxicological Sciences||High sensitivity of testicular function to titanium nanoparticles pubmed:28496042|
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|Anticancer Research||Transdermal Delivery of Luteinizing Hormone-releasing Hormone with Chitosan Microneedles: A Promising Tool for Androgen Deprivation Therapy pubmed:29187457|
|DARU Journal of Pharmaceutical Sciences||Beneficial effects of Heqi san on rat model of polycystic ovary syndrome through the PI3K/AKT pathway pubmed:29020999|
|Reproduction.||Effect of controlled ovarian hyperstimulation on puberty and estrus in mice offspring pubmed:28687593|
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|Environmental Pollution||Prenatal exposure to polychlorinated biphenyl and umbilical cord hormones and birth outcomes in an island population Pubmed:29525625|
|Toxicological Sciences||Impact of Perfluorooctane Sulfonate on Reproductive Ability of Female Mice through Suppression of Estrogen Receptor a-Activated Kisspeptin Neurons 10.1093:toxsci|
|Frontiers in Molecular Neuroscience||Impact of Triclosan on Female Reproduction through Reducing Thyroid Hormones to Suppress Hypothalamic Kisspeptin Neurons in Mice Pubmed:29403355|
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|Catalog No.||Related products for research use of Rattus norvegicus (Rat) Organism species||Applications (RESEARCH USE ONLY!)|
|RPA441Ra01||Recombinant Luteinizing Hormone (LH)||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAA441Ra01||Polyclonal Antibody to Luteinizing Hormone (LH)||WB; IHC; ICC; IP.|
|LAA441Ra71||Biotin-Linked Polyclonal Antibody to Luteinizing Hormone (LH)||WB; IHC; ICC.|
|CEA441Ra||ELISA Kit for Luteinizing Hormone (LH)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|CCA441Ra||CLIA Kit for Luteinizing Hormone (LH)||Chemiluminescent immunoassay for Antigen Detection.|