CLIA Kit for Luteinizing Hormone (LH)
ICSH; Lutropin; Interstitial Cell Stimulating Hormone
- Product No.CCA441Ra
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- Sample Typeserum, plasma and other biological fluids
- Test MethodCompetitive Inhibition
- Assay Length2h
- Detection Range195-50,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 70pg/mL.
- DownloadInstruction Manual
96T*5 96T*10 96T*100
For more details, please contact local distributors! US$ 760
For more details, please contact local distributors! US$ 3420
For more details, please contact local distributors! US$ 6460
For more details, please contact local distributors! US$ 53200
For more details, please contact local distributors!
This assay has high sensitivity and excellent specificity for detection of Luteinizing Hormone (LH).
No significant cross-reactivity or interference between Luteinizing Hormone (LH) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Luteinizing Hormone (LH) and the recovery rates were calculated by comparing the measured value to the expected amount of Luteinizing Hormone (LH) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Luteinizing Hormone (LH) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Luteinizing Hormone (LH) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Luteinizing Hormone (LH) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1||Assay Diluent A||1×12mL|
|Detection Reagent B||1×260µL||Assay Diluent B||1×12mL|
|Reagent Diluent||1×300µL||Substrate B||1×2mL|
|Substrate A||1×10mL||Instruction manual||1|
|Wash Buffer (30 × concentrate)||1×20mL|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
7. Read RLU value immediately.
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|International Journal of Clinical and Experimental Medicine||Studies of the pharmacology of 17α-ethynyl-androst-5-ene-3β,7β,17β-triol, a synthetic anti-inflammatory androstene Ijcem: IJCEM1102004|
|Biochemical Journal||PPARγ co-activator-1α co-activates steroidogenic factor 1 to stimulate the synthesis of luteinizing hormone and aldosterone PubMed: 21108604|
|Molecular and Cellular Endocrinology||Effects of genistein and estrogen receptor subtype-specific agonists in ArKO mice following different administration routes ScienceDirect: S0303720709004043|
|Asian Journal of Andrology||Brief maternal exposure of rats to the xenobiotics dibutyl phthalate or diethylstilbestrol alters adult-type Leydig cell development in male offspring PubMed: 23314658|
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|Andrologia||Dose‐and time‐dependent effects of Garcinia kola seed extract on sexual behaviour and reproductive parameters in male Wistar rats PubMed: 26123866|
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|Biology of Reproduction||Maternal High-Fat Diet Induced Loss of Fetal Oocytes Is Associated with Compromised Follicle Growth in Adult Rat Offspring Pubmed:26962114|
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|Int J Mol Sci.||Overexpression of PRL7D1 in Leydig Cells Causes Male Reproductive Dysfunction in Mice Pubmed:26771609|
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|Toxicological Sciences||Exposure of pregnant mice to perfluorobutanesulfonate causes hypothyroxinemia and developmental abnormalities in female offspring pubmed:27803384|
|Andrologia||Effects of treatment with Hypoxis hemerocallidea extract on sexual behaviour and reproductiveparameters in male rats. pubmed:28000943|
|Biology of Reproduction||Maternal High-Fat Diet-Induced Loss of Fetal Oocytes Is Associated with Compromised Follicle Growth in Adult Rat Offspring. pubmed:26962114|
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|Int J Pharm.||New gonadotropin-releasing hormone glycolipids with direct antiproliferative activity and gonadotropin-releasing potency. pubmed:28232269|
|Catalog No.||Related products for research use of Rattus norvegicus (Rat) Organism species||Applications (RESEARCH USE ONLY!)|
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