Active Fibronectin Type III Domain Containing Protein 5 (FNDC5)
FRCP2; Irisin; Fibronectin type III repeat-containing protein 2
- Product No.APN576Hu61
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Buffer FormulationPBS, pH7.6, containing 5% trehalose.
- Traits Freeze-dried powder
- Purity> 97%
- Isoelectric Point5.0
- ApplicationsCell culture; Activity Assays.
- DownloadInstruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
For more details, please contact local distributors! US$ 675
For more details, please contact local distributors! US$ 1350
For more details, please contact local distributors! US$ 4050
For more details, please contact local distributors! US$ 10125
For more details, please contact local distributors!
Fibronectin type III domain-containing protein 5, the precursor of irisin, is a protein that is encoded by the FNDC5 gene. It was reported that FNDC5 significantly decreased cell number, migration and viability through apoptosis in malignant MDA-MB-231 cells. Thus MDA-MB-231 cells were seeded overnight at a density of 5,000 cells/well, and treated with or without various concentrations of FNDC5 for 48h, then MDA-MB-231 cells were observed by inverted microscope and cell viability was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10µL of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450nm using a microplate reader after incubating the plate for 1-4 hours at 37oC.<br/>Cell apoptosis of MDA-MB-231 cells after incubation with FNDC5 for 48h observed by inverted microscope was shown in Figure 1.<br/>(A) MDA-MB-231 cells cultured in DMEM, stimulated with 4nM FNDC5 for 48h; <br/>(B) Unstimulated MDA-MB-231 cells cultured in DMEM for 48h.<br/>Figure. Cell apoptosis of MDA-MB-231 cells after stimulated with FNDC5.
The dose-effect curve of FNDC5 was shown in Figure 2. It was obvious that FNDC5 significantly decreased cell viability of MDA-MB-231 cells. The ED50 for this effect is typically 1.35~12.52ug/mL.<br/>Figure. The dose-effect curve of FNDC5 on MDA-MB-231 cells.
Reconstitute in PBS (pH7.6) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
- BCA Protein Quantification Kit
- Monoclonal Antibody Customized Service
- Polyclonal Antibody Customized Service
- Protein Activity Test Experiment Service
- Electrophoretic Mobility Shift Assay (EMSA) Experiment Service
- PBS Buffer
- Lentivirus Packaging Experiment Service
- Adenovirus Packaging Experiment Service
- Real Time PCR Experimental Service
|Diabetes Research and Clinical Practice||Serum irisin levels in new-onset type 2 diabetes PubMed: 23369227|
|Journal of diabetes and its complications||Lower circulating irisin is associated with type 2 diabetes mellitus Pubmed: 23619195|
|Horm Metab Res.||Inconsistency in circulating irisin levels: what is really happening Pubmed:24459033|
|Int J Obes (Lond).||Leptin administration activates irisin-induced myogenesis via nitric oxide-dependent mechanisms, but reduces its effect on subcutaneous fat browning in mice Pubmed:25199621|
|Molecular and Cellular Endocrinology||Fenofibrate (PPARalpha agonist) induces beige cell formation in subcutaneous white adipose tissue from diet-induced male obese mice Pubmed:25576856|
|journal of diabetes||Lower irisin level in patients with type 2 diabetes mellitus: A case-control study and meta-analysis PubMed: 25494632|
|The Journal of Maternal-Fetal & Neonatal Medicine||Maternal circulating levels of irisin in intrahepatic cholestasis of pregnancy PubMed: 26689349|
|Hormone and Metabolic Research||Lower Circulating Irisin Level in Patients with Diabetes Mellitus: A Systematic Review and Meta-Analysis Pubmed:27300472|
|Nature Reviews Endocrinology||Physiology and role of irisin in glucose homeostasis. pubmed:28211512|
|Journal of Molecular and Cellular Cardiology||Irisin improves perivascular adipose tissue dysfunction via regulation of the heme oxygenase-1/adiponectin axis in diet-induced obese mice pubmed:27638193|
|Biochemical and Biophysical Research Communications||Irisin stimulates cell proliferation and invasion by targeting the PI3K/AKT pathway in human hepatocellular carcinoma pubmed:28867187|
|PLoS One||Differential actions of PPAR-α and PPAR-β/δ on beige adipocyte formation: A study in the subcutaneous white adipose tissue of obese male mice Pubmed:29351550|
|International Brazilian Journal of Urology||The Diagnostic Value of FNDC5/Irisin in Renal Cell Cancer. Pubmed:29522296|