≡

Active 17-Beta-Hydroxysteroid Dehydrogenase Type 3 (HSD17b3)

SDR12C2; EDH17B3; Short Chain Dehydrogenase/Reductase Family 12C,Member 2; Testosterone 17-Beta-Dehydrogenase 3; Testicular 17-beta-hydroxysteroid dehydrogenase

ACTIVITY TEST

17-Beta-Hydroxysteroid Dehydrogenase Type 3 (HSD17b3) belongs to the HSD17B family with NAD(P)H/NAD(P) -dependent oxidoreductase activity that catalyzes the interconversion between 17-ketosteroids and 17-hydroxysteroids to maintain the balance between less potent (17-keto) and more potent (17β-hydroxy) forms of estrogens and androgens. This isoform of 17 beta-hydroxysteroid dehydrogenase is expressed predominantly in the testis and catalyzes the conversion of androstenedione to testosterone. 3-oxo-5-alpha-steroid 4-dehydrogenase 2 (SRD5a2 ) is a enzyme which plays a central role in sexual differentiation and androgen physiology. A functional ELISA assay was conducted to detect the interaction of recombinant human HSD17b3 and recombinant human SRD5a2. Briefly, HSD17b3 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to SRD5a2-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-HSD17b3 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant human HSD17b3 and recombinant human SRD5a2 was shown in Figure 1, the EC50 for this effect is 27.6 ng/mL.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Journal of Steroid Biochemistry and Molecular Biology Evaluation of 17β-hydroxysteroid Dehydrogenase Activity using Androgen Receptor-mediated Transactivation Pubmed: 31614207
Animals (Basel) Analyses of Molecular Characteristics and Enzymatic Activities of Ovine HSD17B3 34679897
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPF173Hu01 Recombinant 17-Beta-Hydroxysteroid Dehydrogenase Type 3 (HSD17b3) Positive Control; Immunogen; SDS-PAGE; WB.
APF173Hu01 Active 17-Beta-Hydroxysteroid Dehydrogenase Type 3 (HSD17b3) Cell culture; Activity Assays.
PAF173Hu01 Polyclonal Antibody to 17-Beta-Hydroxysteroid Dehydrogenase Type 3 (HSD17b3) WB; IHC; ICC; IP.
MAF173Hu21 Monoclonal Antibody to 17-Beta-Hydroxysteroid Dehydrogenase Type 3 (HSD17b3) WB; IHC; ICC; IP.
SEF173Hu ELISA Kit for 17-Beta-Hydroxysteroid Dehydrogenase Type 3 (HSD17b3) Enzyme-linked immunosorbent assay for Antigen Detection.
LMF173Hu Multiplex Assay Kit for 17-Beta-Hydroxysteroid Dehydrogenase Type 3 (HSD17b3) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.