Active Tumor Necrosis Factor Receptor Superfamily, Member 14 (TNFRSF14)

CD270; TR2; ATAR; HVEA; HVEM; LIGHTR; Herpesvirus Entry Mediator; Herpes virus entry mediator A; Tumor necrosis factor receptor-like 2


TNFRSF14 (Tumor necrosis factor receptor superfamily member 14) belongs to the tumor necrosis factor receptor superfamily. TNFRSF14 functions in signal transduction pathways that activate inflammatory and inhibitory T-cell immune response. It binds herpes simplex virus (HSV) viral envelope glycoprotein D (gD), mediating its entry into cells. A binding ELISA assay was conducted to detect the association of TNFRSF14 with TNFa. Briefly, TNFRSF14 were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL TNFRSF14 were then transferred to TNFa-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-TNFRSF14 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of TNFRSF14 and TNFa was shown in Figure 1, and this effect was in a dose dependent manner.


Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.


Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.


The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.



Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPD926Hu01 Recombinant Tumor Necrosis Factor Receptor Superfamily, Member 14 (TNFRSF14) Positive Control; Immunogen; SDS-PAGE; WB.
APD926Hu01 Active Tumor Necrosis Factor Receptor Superfamily, Member 14 (TNFRSF14) Cell culture; Activity Assays.
PAD926Hu01 Polyclonal Antibody to Tumor Necrosis Factor Receptor Superfamily, Member 14 (TNFRSF14) WB; IHC; ICC; IP.
FAD926Hu01 Anti-Tumor Necrosis Factor Receptor Superfamily, Member 14 (TNFRSF14) Polyclonal Antibody Flow cytometry.