Active Platelet Derived Growth Factor D (PDGFD)
PDGF-D; IEGF; SCDGF-B; Spinal Cord Derived Growth Factor B; Iris-expressed growth factor; Platelet-derived growth factor D, receptor-binding form/latent form
- Product No.APC919Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
- Traits Freeze-dried powder
- Purity> 90%
- Isoelectric Point8.4
- ApplicationsCell culture; Activity Assays.
- DownloadInstruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
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Platelet-derived growth factor D (PDGFD) is a protein that in humans is encoded by the PDGFD gene. The protein encoded by this gene is a member of the platelet-derived growth factor family. PDGF plays a significant role in blood vessel formation, the growth of blood vessels from already-existing blood vessel tissue, mitogenesis. PDGF also plays a role in embryonic development, cell proliferation, cell migration, and angiogenesis. Besides, Macrophage Erythroblast Attacher (MAEA) has been identified as an interactor of PDGFD, thus a binding ELISA assay was conducted to detect the interaction of recombinant human PDGFD and recombinant human MAEA. Briefly, PDGFD were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μL were then transferred to MAEA-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-PDGFD pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were
incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of PDGFD and MAEA was shown in Figure 1, and this effect was in a dose dependent manner.
Figure. The binding activity of PDGFD with MAEA.
Figure. Western Blot
Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
- BCA Protein Quantification Kit
- Monoclonal Antibody Customized Service
- Polyclonal Antibody Customized Service
- Protein Activity Test Experiment Service
- Electrophoretic Mobility Shift Assay (EMSA) Experiment Service
- PBS Buffer
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- Adenovirus Packaging Experiment Service
- Real Time PCR Experimental Service
|Marmara Pharmaceutical Journal||In vitro gene silencing effect of chitosan/shRNA PDGF-D nanoparticles in breast cancer DOI: 10.12991/mpj.2017.21|
|Catalog No.||Related products for research use of Homo sapiens (Human) Organism species||Applications (RESEARCH USE ONLY!)|
|RPC919Hu01||Recombinant Platelet Derived Growth Factor D (PDGFD)||Positive Control; Immunogen; SDS-PAGE; WB.|
|APC919Hu01||Active Platelet Derived Growth Factor D (PDGFD)||Cell culture; Activity Assays.|
|PAC919Hu01||Polyclonal Antibody to Platelet Derived Growth Factor D (PDGFD)||WB; IHC; ICC; IP.|
|MAC919Hu22||Monoclonal Antibody to Platelet Derived Growth Factor D (PDGFD)||WB; IHC; ICC; IP.|
|SEC919Hu||ELISA Kit for Platelet Derived Growth Factor D (PDGFD)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|SCC919Hu||CLIA Kit for Platelet Derived Growth Factor D (PDGFD)||Chemiluminescent immunoassay for Antigen Detection.|