Active Asporin (ASPN)

PLAP1; SLRR1C; Periodontal ligament-associated protein 1


Asporin (ASPN) is a protein belongs to a family of leucine-rich repeat (LRR) proteins associated with the cartilage matrix. The protein negatively regulates chondrogenesis in the articular cartilage and periodontal ligament (PDL) differentiation, inhibits BMP2-induced cytodifferentiation of PDL cells and also nhibits the interaction between TGFB1 and TGF-beta receptor type II in the presence of heparin/heparan sulfate in vitro. Besides, Transforming Growth Factor Beta 2 (TGFb2) has been identified as an interactor of ASPN, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse ASPN and recombinant mouse TGFb2. Briefly, ASPN were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μL were then transferred to TGFb2-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-ASPN pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of ASPN and TGFb2 was shown in Figure 1, and this effect was in a dose dependent manner.
Figure. The binding activity of ASPN with TGFb2.


Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.


Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.


The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.



Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPC321Mu02 Recombinant Asporin (ASPN) Positive Control; Immunogen; SDS-PAGE; WB.
APC321Mu01 Active Asporin (ASPN) Cell culture; Activity Assays.
RPC321Mu01 Recombinant Asporin (ASPN) Positive Control; Immunogen; SDS-PAGE; WB.
PAC321Mu02 Polyclonal Antibody to Asporin (ASPN) WB; IHC; ICC; IP.
PAC321Mu01 Polyclonal Antibody to Asporin (ASPN) WB; IHC; ICC; IP.
SEC321Mu ELISA Kit for Asporin (ASPN) Enzyme-linked immunosorbent assay for Antigen Detection.