Active Asporin (ASPN)
PLAP1; SLRR1C; Periodontal ligament-associated protein 1
- Product No.APC321Mu01
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
- Traits Freeze-dried powder
- Purity> 95%
- Isoelectric Point8.8
- ApplicationsCell culture; Activity Assays.
- DownloadInstruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
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Asporin (ASPN) is a protein belongs to a family of leucine-rich repeat (LRR) proteins associated with the cartilage matrix. The protein negatively regulates chondrogenesis in the articular cartilage and periodontal ligament (PDL) differentiation, inhibits BMP2-induced cytodifferentiation of PDL cells and also nhibits the interaction between TGFB1 and TGF-beta receptor type II in the presence of heparin/heparan sulfate in vitro. Besides, Transforming Growth Factor Beta 2 (TGFb2) has been identified as an interactor of ASPN, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse ASPN and recombinant mouse TGFb2. Briefly, ASPN were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μL were then transferred to TGFb2-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-ASPN pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of ASPN and TGFb2 was shown in Figure 1, and this effect was in a dose dependent manner.
Figure. The binding activity of ASPN with TGFb2.
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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|Catalog No.||Related products for research use of Mus musculus (Mouse) Organism species||Applications (RESEARCH USE ONLY!)|
|RPC321Mu02||Recombinant Asporin (ASPN)||Positive Control; Immunogen; SDS-PAGE; WB.|
|APC321Mu01||Active Asporin (ASPN)||Cell culture; Activity Assays.|
|RPC321Mu01||Recombinant Asporin (ASPN)||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAC321Mu02||Polyclonal Antibody to Asporin (ASPN)||WB; IHC; ICC; IP.|
|PAC321Mu01||Polyclonal Antibody to Asporin (ASPN)||WB; IHC; ICC; IP.|
|SEC321Mu||ELISA Kit for Asporin (ASPN)||Enzyme-linked immunosorbent assay for Antigen Detection.|