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Active Interleukin 20 (IL20)

IL10D; IL10-D; ZCYTO10; Cytokine Zcyto10

ACTIVITY TEST

IL20 (Interleukin-20) is a cytokine structurally related to interleukin 10, which is produced by activated keratinocytes and monocytes. It is accepted that IL20 regulates proliferation and differentiation of keratinocytes during inflammation, particularly inflammation associated with the skin. Thus, proliferation assay of IL20 was conducted using ECV-304 cells. Briefly, ECV-304 cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard 1640 prior to the addition of various concentrations of IL20. After incubated for 48h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10µL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37°C. Proliferation of ECV-304 cells after incubation with IIL20 for 48h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with human recombinant IL20 for 48h. The result was shown in Figure 2. It was obvious that human IL20 significantly decreased cell viability of ECV-304 cells.

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Molecular Oncology The tumor promoting activity of the EP4 receptor for prostaglandin E2 in murine skin Pubmed:25034079
Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease IL-20 promotes hypoxia/reoxygenation-induced mitochondrial dysfunction and apoptosis in cardiomyocytes by upregulating oxidative stress by activating the PKC …
Theranostics IL20RA signaling enhances stemness and promotes the formation of an immunosuppressive microenvironment in breast cancer 33456560
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPC058Hu01 Recombinant Interleukin 20 (IL20) Positive Control; Immunogen; SDS-PAGE; WB.
APC058Hu01 Active Interleukin 20 (IL20) Cell culture; Activity Assays.
PAC058Hu01 Polyclonal Antibody to Interleukin 20 (IL20) WB; IHC; ICC; IP.
MAC058Hu22 Monoclonal Antibody to Interleukin 20 (IL20) WB; IHC; ICC; IP.
RAC058Hu21 Recombinant Antibody to Interleukin 20 (IL20) WB; IF; ICC; IHC; IP; FCM.
SEC058Hu ELISA Kit for Interleukin 20 (IL20) Enzyme-linked immunosorbent assay for Antigen Detection.
SCC058Hu CLIA Kit for Interleukin 20 (IL20) Chemiluminescent immunoassay for Antigen Detection.
LMC058Hu Multiplex Assay Kit for Interleukin 20 (IL20) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.