Active Hepcidin (Hepc)

HAMP; HFE2B; PLTR; LEAP1; Hepcidin Antimicrobial Peptide; Liver-expressed antimicrobial peptide 1; Putative liver tumor regressor

ACTIVITY TEST

Figure. The binding activity of Hepc with FPN.
Hepcidin (Hepc) is a regulator of iron metabolism. Hepcidin inhibits iron transport by binding to the iron export channel ferroportin which is located on the basolateral surface of gut enterocytes and the plasma membrane of reticuloendothelial cells (macrophages). Hepcidin ultimately breaks down the transporter protein in the lysosome. Inhibiting ferroportin prevents iron from being exported and the iron is sequestered in the cells. Besides, Ferroportin (FPN) has been identified as an interactor of Hepc, thus a binding ELISA assay was conducted to detect the interaction of recombinant human Hepc and recombinant human FPN. Briefly, Hepc were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100µL were then transferred to FPN-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-Hepc pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of Hepc and FPN was shown in Figure 1, and this effect was in a dose dependent manner.

USAGE

Reconstitute in 10mM PBS (pH7.6) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
World J Gastroenterol. Hepcidin expression in colon during trinitrobenzene sulfonic acid-induced colitis in rats Pubmed: 24764672
International Journal of Advanced Research Modulations of serum hepcidin, IL6 and iron status by different exercise regimens in ovarectomized rats Com:Source
Neurochemical Journal Serum iron metabolism markers including hepcidin in multiple sclerosis patients Springer:Source
Afro-Egypt J Infect Endem Dis Monitoring Hepcidin Level in Chronic Hepatitis C Virus Patients during Therapy Zu:Source
Gastroenterology Gluconeogenic signals regulate iron homeostasis via hepcidin in mice Pubmed:Pmc3989026
J Card Fail. Inappropriate expression of hepcidin by liver congestion contributes to anemia and relative iron deficiency Pubmed:24440572
Food Funct. The action of JAK, SMAD and ERK signal pathways on hepcidin suppression by polysaccharides from Angelica sinensis in rats with iron deficiency anemia Pubmed:24752529
Free Radic Res. Changes in skeletal muscle iron metabolism outpace amyotrophic lateral sclerosis onset in transgenic rats bearing the G93A hmSOD1 gene mutation Pubmed:25175826
Saudi J Kidney Dis Transpl. Hepcidin and regulation of iron homeostasis in maintenance hemodialysis patients Pubmed:25193892
J Endocrinol Invest.? The interplay among iron metabolism, endothelium and inflammatory cascade in dysmetabolic disorders Pubmed:25245337
Gastroenterology Research and Practice An Evaluation of the Correlation between Hepcidin Serum Levels and Disease Activity in Inflammatory Bowel Disease Pubmed:25628652
Clin Lab. Serum hepcidin levels in Bulgarian population. Pubmed:25651734
The Tohoku Journal of Experimental Medicine Liver Congestion in Heart Failure Contributes to Inappropriately Increased Serum Hepcidin despite Anemia Pubmed:25742771
Cancer Res Hepcidin regulation in prostate and its disruption in prostate cancer PubMed: 25858146
Biochem Biophys Res Commun B-cell translocation gene 2 promotes hepatic hepcidin production via induction of Yin Yang 1 PubMed: 25839654
Blood Purif Effect of a More Permeable Dialysis Membrane on ESA Resistance in Hemodialysis Patients - A Pilot Investigation PubMed: 26536083
Asian Journal of Clinical Nutrition Urinary Hepcidin in Diagnosis of Iron Deficiency Anemia Openview: E01Ad7C4A9109Ed5Fadb03Ec206D7Bdc
Front Psychol Ferrous sulfate, but not iron polymaltose complex, aggravates local and systemic inflammation and oxidative stress in dextran sodium sulfate-induced colitis in rats PubMed: 25852597
Biol Trace Elem Res Iron Supplementation Attenuates the Inflammatory Status of Anemic Piglets by Regulating Hepcidin PubMed: 25774043
APS Journals Kupffer cell depletion protects against the steatosis, but not the liver damage, induced by marginal copper, high fructose diet in male rats. PubMed: 25813056
European Journal of Haematology Hepcidin in tumor‐related iron deficiency anemia and tumor‐related anemia of chronic disease: pathogenic mechanisms and diagnosis PubMed: 24954786
WJA The Effects of Treatment on Serum Hepcidin and Iron Homeostasis in HIV-1-Infected In-dividuals Journal: 59107
Nutrition, Metabolism and Cardiovascular Diseases Hepcidin and iron metabolism associated with cardiometabolic risk factors in children: A case-control study. Pubmed:27139516
Food Funct The effects of polysaccharides from the root of Angelica sinensis on tumor growth and iron metabolism in H22-bearing mice Pubmed:26757699
Antioxid Redox Signal. Hydrogen sulfide attenuates inflammatory hepcidin by reducing IL-6 secretion and promoting SIRT1-mediated STAT3 deacetylation Pubmed:26154696
BioMed Research International The Impact of Acute Matriptase Inhibition in Hepatic Inflammatory Models. pubmed:27642598
Frontiers in Neuroscience In Absence of the Cellular Prion Protein, Alterations in Copper Metabolism and Copper-Dependent Oxidase Activity Affect Iron Distribution. pubmed:27729845
Experimental Biology and Medicine Effects of iron supplementation in mice with hypoferremia induced by obesity pubmed:27439539
Nutrition, Metabolism and Cardiovascular Diseases Hepcidin and iron metabolism associated with cardiometabolic risk factors in children: A case–control study pubmed:27139516
PLOS ONE S-Propargyl-Cysteine, a Novel Hydrogen Sulfide Donor, Inhibits Inflammatory Hepcidin and Relieves Anemia of Inflammation by Inhibiting IL-6/STAT3 Pathway. pubmed:27649298
Endocrine connections Hepcidin links gluco-toxicity to pancreatic beta cell dysfunction by inhibiting Pdx-1 expression pubmed:28179377
Maternal and cord blood hepcidin levels based on gestational weeks in term and preterm infants publication:305921433_Maternal_and_cord_blood_hepc
Oxidative Medicine and Cellular Longevity Acidic Polysaccharide from Angelica sinensis Reverses Anemia of Chronic Disease Involving the Suppression of Inflammatory Hepcidin and NF-κB Activation pubmed:29147463
The Royal Society of Chemistry Silkworm feces extract improves iron deficiency anemia via suppressing hepcidin expression and promoting iron-regulatory proteins expression DOI: 10.1039/C7RA09576G
Journal of Inflammation Evaluation of a competitive hepcidin ELISA assay in the differential diagnosis of iron deficiency anaemia with concurrent inflammation and anaemia of inflammation in elderly patients 10.1186:s12950-017-0166-3
Antioxidants and Redox Signaling AMPK serves as a Therapeutic Target against Anemia of Inflammation pubmed:27923278
NutriciónHospitalaria Micronutrient supplementation in gastric bypass surgery: prospective study on infl ammation and iron metabolism in premenopausal women 10.20960/nh.1162
The Japanese Society of Hematology Hepcidin suppression in β‑thalassemia is associated with the down‑regulation of atonal homolog 8 pubmed:28405918
Tuberculosis Modulation of iron status biomarkers in tuberculosis-diabetes co-morbidity 10.1016/j.tube.2017.11.011
Oman Medical Journal The Role of Insulin Therapy in Correcting Hepcidin Levels in Patients with Type 2 Diabetes Mellitus. pubmed:28584599
Cellular Physiology and Biochemistry The Effects of Angelica Sinensis Polysaccharide on Tumor Growth and Iron Metabolism by Regulating Hepcidin in Tumor-Bearing Mice Pubmed:29843136
Cellular Microbiology  inhibits ferroportin translation by modulating FBXL5‐IRP2 axis for its growth within host macrophages Pubmed:29470856
Journal of Histochemistry & Cytochemistry Acute and Chronic Iron Overloading Differentially Modulates the Expression of Cellular Iron-homeostatic Molecules in Normal Rat Kidney Pubmed:29873589
Clinical and Experimental Medicine Increased NGAL level associated with iron store in chronic kidney disease with anemia Pubmed:29909502
Hematology Relationship between obesity and iron deficiency anemia: is there a role of hepcidin? Pubmed:29318945
Hepcidin serum levels in HCV chronically mono-infected naïve patients, compared to healthy individuals 77856
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