Active Hemoglobin (HB)

Hgb; Haemoglobin; Heterotetramer(αβ)2

ACTIVITY TEST

Hemoglobin (HB) is a protein in red blood cells which contains iron. It is used to transport oxygen around the human body. Hemoglobin is found in the red blood cells of almost all vertebrates. Hemoglobin has peroxidase activity. In the presence of hydrogen peroxide, hemoglobin can catalyze the substrates 3,3 ', 5,5' - tetramethylbenzidine (TMB) to produce a blue compound which has a maximum absorption at 595 nm. Thus, the activity of native chinese hamster hemoglobin was measured by its ability to catalyze the substrates of TMB. The reaction was performed in PBS, pH 7.4 ( Assay Buffer), ainitiated by addition 40 μL of various concentrations of HB (diluted by Assay Buffer) to 160 µL of TMB. Incubated at 37 ℃ for 20min, then read at a wavelength of 595 nm immediately. The result was shown in figure 1, and the OD value was in a linear relationship with the concentration of HB.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology Effects of ectoine on behavioural, physiological and biochemical parameters of Daphnia magna Pubmed:25460046
PLoS One. Comparative Proteomics Identifies Host Immune System Proteins Affected by Infection with Mycobacterium bovis Pubmed:27027307
Parasites & Vectors The intracellular bacterium Anaplasma phagocytophilum selectively manipulates the levels of vertebrate host proteins in the tick vector Ixodes scapularis. pubmed:27561965
Proteomics. Clinical Applications Elevated levels of protein AMBP in cerebrospinal fluid of women with preeclampsia compared to normotensive pregnant women. pubmed:27615121
Molecular nutrition & food research Chicory inulin ameliorates type 2 diabetes mellitus and suppresses JNK and MAPK pathways in vivo and in vitro pubmed:28105758
Frontiers in Immunology Oxidized Hemoglobin Is Antigenic and Immunogenic in Lupus pubmed:28694810
新規人工酸素運搬体HemoAct を用いた脳虚血再灌流障害治療開発に関する研究 :
Acta Neuropathologica Communications Mural cell-derived laminin-α5 plays a detrimental role in ischemic stroke Pubmed: 30777135
Angiogenesis Decylubiquinone suppresses breast cancer growth and metastasis by inhibiting angiogenesis via the ROS/p53/BAI1 signaling pathway Pubmed: 32020421
Food Bioscience Water-extractable zinc protoporphyrin IX in Parma ham predominantly exists as complexes with hemoglobin and myoglobin
Photochem Photobiol Effects of Human Serum Albumin on the Fluorescence Intensity and Tumor Imaging Properties of IR‐780 Dye 34687567
Catalog No. Related products for research use of Hamster (Chinese hamster) Organism species Applications (RESEARCH USE ONLY!)
NPB409Ha01 Native Hemoglobin (HB) Positive Control; Immunogen; SDS-PAGE; WB.
APB409Ha01 Active Hemoglobin (HB) Cell culture; Activity Assays.
PAB409Ha01 Polyclonal Antibody to Hemoglobin (HB) WB; IHC; ICC; IP.
LAB409Ha71 Biotin-Linked Polyclonal Antibody to Hemoglobin (HB) WB; IHC; ICC.