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Active Superoxide Dismutase 2, Mitochondrial (SOD2)

IPO-B; MNSOD; Mn-SOD

ACTIVITY TEST

Extracellular superoxide dismutase [Cu-Zn] is an enzyme that in humans is encoded by the SOD2 gene. This gene encodes a member of the superoxide dismutase (SOD) protein family. SODs are antioxidant enzymes that catalyze the dismutation of two superoxide radicals into hydrogen peroxide and oxygen. Acroding to the report, in a weakly alkaline buffer solution (pH=8.2) with N-tris(hydroxymethyl)amino methane-HCL, pyrogallol can occur autoxidation in the air, then SOD can inhibit this reaction. Thus, we use this way to measued the activity of recombinant rat SOD2. The reaction was performed in adding 8 μl 5 mmol/L pyrogallol to 200 μl 50mmol/L Tris-HCl, rapidly mixing at 25 ℃, then read at 325 nm (using 50mmol/L Tris-HCl as blank control) in kinetic mode for 3 minutes using a microplate reader controlling the pyrogallol autoxidation rate at 0.70 OD/min. Different concentrations of recombinant rat SOD2 were added into 200 μl 50 mmol/L Tris-HCl, incubated for 20 min at 25 ℃, then adding 8 μl 5 mmol/L pyrogallol to each well, rapidly mixing and read at 325 nm in kinetic mode for 3 minutes. Under these conditions, the enzyme amount of 50% inhibition of pyrogallol autooxidation per minute is defined as a unit. The specific activity of recombinant rat SOD2 is 390 U/mg.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Oral Disease Antioxidant profile of salivary glands in high fat diet-induced insulin resistance rats Pubmed: 24106991
AGE Consequences of age on ischemic wound healing in rats: altered antioxidant activity and delayed wound closure Pubmed:24443098
Anticancer Research Determination of Gene Expression and Serum Levels of MnSOD and GPX1 in Colorectal Cancer Pubmed:25550558
Arch Oral Biol Antioxidant profile, carbonyl and lipid oxidation markers in the parotid and submandibular glands of rats in different periods of streptozotocin induced diabetes PubMed: 26143097
J Oral Pathol Med Impact of morbid obesity and bariatric surgery on antioxidant/oxidant balance of the unstimulated and stimulated human saliva PubMed: 26608886
Free Radic Biol Med. DDAH1 deficiency promotes intracellular oxidative stress and cell apoptosis via a miR-21-dependent pathway in mouse embryonic fibroblasts. Pubmed:26806551
Basic Research in Cardiology Cardiomyocyte dimethylarginine dimethylaminohydrolase1 attenuates left-ventricular remodeling after acute myocardial infarction: involvement in oxidative stress and … Pubmed:29892894
Journal of Pharmacological Sciences Improved endogenous epoxyeicosatrienoic acid production mends heart function via increased PGC 1α-mitochondrial functions in metabolic syndrome Pubmed: 30342783
Advances in Medical Sciences Selected elements of extracellular matrix of the skin in diabetes and insulin resistance Pubmed: 31146169
PHARMACOLOGICAL RESEARCH A Machine Learning-driven Study Indicates Emodin Improves Cardiac Hypertrophy by Modulation of Mitochondrial SIRT3 Signaling Pubmed: 32135248
Biomolecules Arachidonic Acid as an Early Indicator of Inflammation during Non-Alcoholic Fatty Liver Disease Development Pubmed: 32751983
Nutrients Attenuation of Oxidative Stress and Inflammatory Response by Chronic Cannabidiol Administration Is Associated with Improved n-6/n-3 PUFA Ratio in the White and?¡­ 34064937
Pharmaceuticals Serum Total SOD Activity and SOD1/2 Concentrations in Predicting All-Cause Mortality in Lung Cancer Patients 34832849
Biochimica et Biophysica Acta-Molecular Basis of Disease α-Lipoic acid ameliorates inflammation state and oxidative stress by reducing the content of bioactive lipid derivatives in the left ventricle of rats fed a high-fat diet Pubmed:35569738
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