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Active Cluster Of Differentiation 26 (CD26)

CD26; ADA; DPPIV; DPP-IV; DPP4; ADABP; ADCP2; TP103; Adenosine Deaminase Complexing Protein 2; T-cell activation antigen CD26; Dipeptidyl Peptidase IV

ACTIVITY TEST

Figure. The binding activity of DPP4 with ECF.
Dipeptidyl peptidase-4 (DPP4), also known as adenosine deaminase complexing protein 2 or cluster of differentiation 26 (CD26), is a protein in humans. DPP4 is an antigenic enzyme expressed on the surface of most cell types and is associated with immune regulation, signal transduction and apoptosis. It is an intrinsic membrane glycoprotein and a serine exopeptidase that cleaves X-proline dipeptides from the N-terminus of polypeptides. Besides, Eosinophil Chemotactic Factor (ECF) has been identified as an interactor of DPP4, thus a binding ELISA assay was conducted to detect the interaction of recombinant human DPP4 and recombinant human ECF. Briefly, DPP4 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to ECF-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-DPP4 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of DPP4 and ECF was shown in Figure 1, and this effect was in a dose dependent manner.

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
hormone and metabolic research Linagliptin Blocks Renal Damage in Type 1 Diabetic Rats by Suppressing Advanced Glycation End Products-Receptor Axis Pubmed: 24710699
Curr Med Chem. MK-0626, a dipeptidyl peptidase-4 inhibitor, improves neovascularization by increasing both the number of circulating endothelial progenitor cells and endothelial nitric oxide synthetase expression. Pubmed:24059225
Diabetes Ghrelin inhibition restores glucose homeostasis in hepatocyte nuclear factor-1alpha (MODY3) deficient mice PubMed: 25979074
The American Journal of the Medical Sciences Expression and Clinical Significance of Serum Dipeptidyl Peptidase IV Chronic Obstructive Pulmonary Disease Pubmed:26992252
Gene Expression of recombinant human α-lactalbumin in milk of transgenic cloned pigs is sufficient to enhance intestinal growth and weight gain of suckling piglets Pubmed:26899869
Applied In Vitro Toxicology Hepatic Spheroids for Long-Term Toxicity Studies aivt.2016.0016
BMJ Open Diab Res Care High circulating plasma dipeptidyl peptidase- 4 levels in non-obese Asian Indians with type 2 diabetes correlate with fasting insulin and LDL-C levels, triceps skinfolds, total intra-abdominal adipose tissue volume and presence of diabetes: a case–control study 10.1136:bmjdrc-2017-000393
Diabetology & Metabolic Syndrome Dipeptidyl peptidase-4 levels are increased and partially related to body fat distribution in patients with familial partial lipodystrophy type 2 pubmed:28450900
Journal of Cellular Biochemistry The effect of CD26‐deficiency on dipeptidyl peptidase 8 and 9 expression profiles in a mouse model of Crohn's disease Pubmed:29693275
Medicine (Baltimore) Rapid detection of urinary soluble intercellular adhesion molecule-1 for determination of lupus nephritis activity Pubmed:29953010
Sci Rep The novel adamantane derivatives as potential mediators of inflammation and neural plasticity in diabetes mice with cognitive impairment Pubmed:35468904
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