≡

Active Carbonic Anhydrase II (CA2)

CA-II; CAII; Car2; CAC; Carbonate dehydratase II; Carbonic anhydrase C

ACTIVITY TEST

Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO2  H2O = HCO3-  H , which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption. CA2 is a cytosolic enzyme with the highest activity among all known CAs. Mutations in the CA2 gene result in the CA II deficiency syndrome, an autosomal recessive disorder that produces osteopetrosis, renal tubular acidosis and cerebral calcification. The activity of recombinant human CA2 was measured by its ability to hydrolyze 4-Nitrophenyl acetate (4-NPA) to 4-Nitrophenol. The reaction was performed in 12.5 mM Tris, 75 mM NaCl, pH 7.5 (assay buffer), initiated by addition 50 μL of various concentrations of CA2 (diluted by assay buffer) to 50 µL of 2 mM substrate 4-NPA (100 mM stock in Acetone, diluted by assay buffer). Incubated at 37℃ for 5min, then read at a wavelength of 400 nm.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Proteomics Clin Appl.  Identification of potential breast cancer markers in nipple discharge by protein profile analysis using two-dimensional nano-liquid chromatography/nanoelectrospray ionization-mass spectrometry. Pubmed:26970563
PROTEOMICS - Clinical Applications Comparative mass spectrometric and immunoassay-based proteome analysis in serum ofDuchenne muscular dystrophy patients. pubmed:26680509
Cell & Molecular Biology Identification of potential breast cancer markers in nipple discharge by protein profile analysis using two‐dimensional nano‐liquid chromatography/nanoelectrospray … doi:10.1002
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
NPA782Hu01 Native Carbonic Anhydrase II (CA2) Positive Control; Immunogen; SDS-PAGE; WB.
RPA782Hu01 Recombinant Carbonic Anhydrase II (CA2) Positive Control; Immunogen; SDS-PAGE; WB.
RPA782Hu02 Recombinant Carbonic Anhydrase II (CA2) Positive Control; Immunogen; SDS-PAGE; WB.
RPA782Hu04 Recombinant Carbonic Anhydrase II (CA2) Positive Control; Immunogen; SDS-PAGE; WB.
APA782Hu01 Active Carbonic Anhydrase II (CA2) Cell culture; Activity Assays.
PAA782Hu01 Polyclonal Antibody to Carbonic Anhydrase II (CA2) WB; IHC; ICC; IP.
PAA782Hu04 Polyclonal Antibody to Carbonic Anhydrase II (CA2) WB; IHC; ICC; IP.
PAA782Hu03 Polyclonal Antibody to Carbonic Anhydrase II (CA2) WB; IHC; ICC; IP.
PAA782Hu02 Polyclonal Antibody to Carbonic Anhydrase II (CA2) WB; IHC; ICC; IP.
LAA782Hu71 Biotin-Linked Polyclonal Antibody to Carbonic Anhydrase II (CA2) WB; IHC; ICC.
MAA782Hu22 Monoclonal Antibody to Carbonic Anhydrase II (CA2) WB; IHC; ICC; IP.
SEA782Hu ELISA Kit for Carbonic Anhydrase II (CA2) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA782Hu Multiplex Assay Kit for Carbonic Anhydrase II (CA2) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSA782Hu01 ELISA Kit DIY Materials for Carbonic Anhydrase II (CA2) Main materials for "Do It (ELISA Kit) Yourself".