Active Macrophage Migration Inhibitory Factor (MIF)
GIF; GLIF; MMIF; Glycosylation-Inhibiting Factor; L-dopachrome isomerase; L-dopachrome tautomerase; Phenylpyruvate tautomerase
- Product No.APA698Mu01
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
- Traits Freeze-dried powder
- Purity> 95%
- Isoelectric Point6.7
- ApplicationsCell culture; Activity Assays.
- DownloadInstruction Manual
200µg 1mg 5mg
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Figure. The binding activity of MIF with MHCDG.
Macrophage migration inhibitory factor (MIF), also known as glycosylation-inhibiting factor (GIF), L-dopachrome isomerase, or phenylpyruvate tautomerase is a protein classified as an inflammatory cytokine. MIF is an important regulator of innate immunity. It involved in cell-mediated immunity, immunoregulation, and inflammation. MIF plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate a role in integrin signaling pathways. Besides, Major Histocompatibility Complex Class II Invariant Chain (MHCDG) has been identified as an interactor of MIF, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse MIF and recombinant mouse MHCDG. Briefly, MIF were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to MHCDG-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-MIF pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of MIF and MHCDG was shown in Figure 1, and this effect was in a dose dependent manner.
Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
|Kidney International||Targeted reduction of advanced glycation improves renal function in obesity PubMed: 21412218|
|The Journal of Immunology||Macrophage Migration Inhibitory Factor Plays a Role in the Regulation of Microfold (M) Cell-Mediated Transport in the Gut Jimmunol: 5673|
|The Journal of Endocrinology||Involvement of exercise-induced macrophage migration inhibitory factor in the prevention of fatty liver disease PubMed: PMC3757527|
|The Journal of Immunology||Role of macrophage migration inhibitory factor in the regulatory T cell response of tumor-bearing mice PubMed: PMC3466372|
|Diabetologia||Deletion of bone-marrow-derived receptor for AGEs (RAGE) improves renal function in an experimental mouse model of diabetes Pubmed:24957662|
|BioMed Research International||The Potential Role of Polymethyl Methacrylate as a New Packaging Material for the Implantable Medical Device in the Bladder PubMed: 25705692|
|Diabetologia||Combined NOX1/4 inhibition with GKT137831 in mice provides dose-dependent reno- and atheroprotection even in established micro- and macrovascular disease. pubmed:28160092|
|Catalog No.||Related products for research use of Mus musculus (Mouse) Organism species||Applications (RESEARCH USE ONLY!)|
|APA698Mu01||Active Macrophage Migration Inhibitory Factor (MIF)||Cell culture; Activity Assays.|
|RPA698Mu02||Recombinant Macrophage Migration Inhibitory Factor (MIF)||Positive Control; Immunogen; SDS-PAGE; WB.|
|RPA698Mu01||Recombinant Macrophage Migration Inhibitory Factor (MIF)||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAA698Mu01||Polyclonal Antibody to Macrophage Migration Inhibitory Factor (MIF)||WB; IHC; ICC; IP.|
|PAA698Mu02||Polyclonal Antibody to Macrophage Migration Inhibitory Factor (MIF)||WB; IHC; ICC; IP.|
|SEA698Mu||ELISA Kit for Macrophage Migration Inhibitory Factor (MIF)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|SCA698Mu||CLIA Kit for Macrophage Migration Inhibitory Factor (MIF)||Chemiluminescent immunoassay for Antigen Detection.|